We narrowed to 30,772 results for: ple
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Plasmid#111946PurposeThis is a shuffle plasmid containing the HSH155 gene and the surrounding genomic regions. It bears a TRP marker for shuffle into a HSH155 deletion strain. It has the Q773A R775A R776A Q778A mutationDepositorInsertHsh155 Q773A R775A R776A Q778A (C4A)
ExpressionBacterial and YeastMutationQ773A R775A R776A Q778AAvailable SinceJune 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRS315Nab3D191stop
Plasmid#110537PurposeExpresses yeast NAB3 using the endogenous promoterDepositorAvailable SinceJune 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
AAV-FLEX-mCherry-FHA1-subT391A-mCherry
Plasmid#104462PurposeCre-dependent, Non-phosphorylatable PKA consensus substrate tagged with mCherryDepositorInsertmCherry-subT391A-FHA1-mCherry
UseAAVTagsmCherryPromoterCAGAvailable SinceJune 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28b+R3(R112C/T180C)C
Plasmid#108194PurposeExpresses PdR-PCNA3(R112C/T180C)-P450cam fusion protein in E. coliDepositorInsertPdR-PCNA3(R112C/T180C)-P450cam fusion protein
TagsHis6 tagExpressionBacterialMutationR112C/T180C(PCNA3)PromoterT7 promoterAvailable SinceApril 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28b+3(R112C/T180C)C
Plasmid#108193PurposeExpresses PCNA3(R112C/T180C)-P450cam fusion protein in E. coliDepositorInsertPCNA3(R112C/T180C)-P450cam fusion protein
TagsHis6 tagExpressionBacterialMutationR112C/T180C(PCNA3)PromoterT7 promoterAvailable SinceApril 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET15b+2(L171C)X
Plasmid#108192PurposeExpresses PdR-PCNA2(L171C)-PdX fusion protein in E. coliDepositorInsertPCNA2(L171C)-PdX fusion protein
TagsHis6 tagExpressionBacterialMutationL171C (PCNA2); C73S/C85S (PdX)PromoterT7 promoterAvailable SinceApril 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET15b+R2(L171C)X
Plasmid#108191PurposeExpresses PdR-PCNA2(L171C)-PdX fusion protein in E. coliDepositorInsertPdR-PCNA2(L171C)-PdX fusion protein
TagsHis6 tagExpressionBacterialMutationL171C (PCNA2); C73S/C85S (PdX)PromoterT7 promoterAvailable SinceApril 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET15b+1(G108C)X
Plasmid#108189PurposeExpresses PCNA1(G108C)-PdX fusion protein in E. coliDepositorInsertPCNA1(G108C)-PdX fusion protein
TagsHis6 tagExpressionBacterialMutationG108C (PCNA1); C73S/C85S (PdX)PromoterT7 promoterAvailable SinceApril 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
UBQ10:sXVE:ATG-3xHA-S11
Plasmid#108238PurposeBinary vectors used for the inducible expression of tripartite split-sfGFP components (3xHA-S11 as control) in plantsDepositorInsertATG-3xHA-S11
UseSynthetic BiologyExpressionPlantPromoterUBQ10:sXVE:Available SinceApril 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGS_C_3xFLAG
Plasmid#101653PurposeExpression of type I signal-peptide containing proteins with C-terminal 3xFLAG-tag in insect cellsDepositorInsertLRP6 Signal peptide, MCS linker, 3xFLAG tag
Tags3xFLAG tagExpressionInsectAvailable SinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pYPQ127B
Plasmid#99883PurposeExpress two TALE-VP64 activator linked by in-frame T2A (encoding Thosea asigna 'self-cleaving' 2A peptide) sequence under 2x35S promoterDepositorInsertTALE-VP64-T2A-TALE-VP64
UseTALENExpressionPlantAvailable SinceMarch 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJFRC81-3xVAS4-Syn21-FLAG-Cerulean-P10
Plasmid#104618PurposeExpresses FLAG tagged Cerulean upon binding of TALE4 to VAS4DepositorInserthsp70-Syn21-FLAG-mCerulean-P10
TagsFLAGExpressionInsectPromoterhsp70Available SinceMarch 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJFRC81-3xVAS1-Syn21-Citrine-HA-P10
Plasmid#104616PurposeExpresses HA tagged Citrine upon binding of TALE1 to VAS1DepositorInsertSyn21-Citrine-HA-P10-MhcSD
TagsHAExpressionInsectPromoterhsp70Available SinceJan. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAFMW-AthHEN1-CM
Plasmid#104981PurposeExpression of FLAG Myc tagged catalytic dead A.thaliana-Hen1 (codon optimised for Drosophila) in Drosophila S2 cellsDepositorAvailable SinceJan. 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T-1 mSesn2 (1190)
Plasmid#66916Purposebacterial expression of mouse Sesn2DepositorAvailable SinceDec. 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDD2024
Plasmid#101368PurposeName: pCBG68-RBPjkΔA. RBPjk binding site A mutation in pCBG68-LANApi (pDD2002).DepositorInsertRBPjk binding site A mutation in pCBG68-LANApi (pDD2002).
UseLuciferaseMutationRBPjk binding site A mutation in pCBG68-LANApi (p…Available SinceDec. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMCh-76
Plasmid#92288PurposePlasmid for expression of NHA-tagged Drosophila melanogaster GW182 CED in D. melanogaster cellsDepositorAvailable SinceDec. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDD2015
Plasmid#101365PurposeName: pCBR-K14ΔTATA. K14 TATA box mutation in pCBR-K14 (pDD2005).DepositorInsertK14 TATA box mutation in pCBR-K14 (pDD2005)
UseLuciferaseMutationK14 TATA box mutation in pCBR-K14 (pDD2005)Available SinceOct. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDD2023
Plasmid#101367PurposeName: pCBR-RBPjkΔB. RBPjk binding site B mutation in pCBR-K14 (pDD2005).DepositorInsertRBPjk binding site B mutation in pCBR-K14 (pDD2005).
UseLuciferaseMutationRBPjk binding site B mutation in pCBR-K14 (pDD200…Available SinceOct. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDD2028
Plasmid#101371PurposeName: pCBR-RBPjkΔA. RBPjk binding site A mutation in pCBR-K14 (pDD2005).DepositorInsertRBPjk binding site A mutation in pCBR-K14 (pDD2005).
UseLuciferaseMutationRBPjk binding site A mutation in pCBR-K14 (pDD200…Available SinceOct. 31, 2017AvailabilityAcademic Institutions and Nonprofits only