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Plasmid#83157PurposeGateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), T35S mutationDepositorInsertKRAS (KRAS Human)
UseGateway entry cloneTagsExpressionMutationT35SPromoterAvailable sinceSept. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
Hs.KRAS4b K147Q
Plasmid#83155PurposeGateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), K147Q mutationDepositorInsertKRAS (KRAS Human)
UseGateway entry cloneTagsExpressionMutationK147QPromoterAvailable sinceSept. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
Hs.KRAS4b K147L
Plasmid#83154PurposeGateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), K147L mutationDepositorInsertKRAS (KRAS Human)
UseGateway entry cloneTagsExpressionMutationK147LPromoterAvailable sinceSept. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
Hs.KRAS4b Y64A
Plasmid#83151PurposeGateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), Y64A mutationDepositorInsertKRAS (KRAS Human)
UseGateway entry cloneTagsExpressionMutationY64APromoterAvailable sinceSept. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
Hs.KRAS4b R68S
Plasmid#83148PurposeGateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), R68S mutationDepositorInsertKRAS (KRAS Human)
UseGateway entry cloneTagsExpressionMutationR68SPromoterAvailable sinceSept. 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
Hs.KRAS4b A146V
Plasmid#83147PurposeGateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), A146V mutationDepositorInsertKRAS (KRAS Human)
UseGateway entry cloneTagsExpressionMutationA146VPromoterAvailable sinceSept. 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
Hs.KRAS4b K117N
Plasmid#83146PurposeGateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), K117N mutationDepositorInsertKRAS (KRAS Human)
UseGateway entry cloneTagsExpressionMutationK117NPromoterAvailable sinceSept. 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
Hs.KRAS4b M188L
Plasmid#83141PurposeGateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), M188L mutationDepositorInsertKRAS (KRAS Human)
UseGateway entry cloneTagsExpressionMutationM188LPromoterAvailable sinceSept. 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
AAV:ITR-U6-sgRNA(Kras)-U6-sgRNA(p53)-U6-sgRNA(Lkb1)-pEFS-Rluc-2A-Cre-shortPA-KrasG12D_HDRdonor-ITR (AAV-KPL)
Plasmid#60224PurposeExpresses Cre recombinase and Renilla luciferase from the EFS promoter and three U6-driven sgRNAs targeting Kras, p53, and Lkb1. Contains KrasG12D HDR donor. AAV backbone.DepositorInsertsUseAAV, CRISPR, Cre/Lox, and Mouse TargetingTagsCre-HA and Rluc-P2AExpressionMammalianMutationPromoterEFS, No promoter, and U6Available sinceOct. 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDNA2.0 6H-TEV-KRAS
Plasmid#111849PurposeExpresses wild type human KRAS (amino acids 1 - 169) in E. coli with amino terminal 6xHIS tag that can be removed with TEV protease.DepositorInsertKRAS (KRAS Human)
UseTags6xHis-tag and TEV protease cleavage sequenceExpressionBacterialMutationPromoterT5Available sinceJune 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
lenti_dCas9-KRAB-MeCP2
Plasmid#122205PurposeBipartite dCas9 repressor (dCas9-KRAB-MeCP2) in a UCOE-SFFV-dCas9 lentiviral vector.DepositorInsertdCas9-KRAB-MeCP2
UseLentiviralTagsKRAB-MeCP2ExpressionMammalianMutationCas9 (D10A, H840A)PromoterAvailable sinceFeb. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLVUT-tTR-KRAB
Plasmid#11651PurposeTet-regulated (Tet-on) lentiviral vector for transgene (hUbiquitin promoter) - AND/OR - shRNA (H1 promoter when subcloned from pLVTHM (Addgene#12247)) - 3rd generationDepositorHas ServiceCloning Grade DNAInserthUbiquitin C, GFP, tTR-KRAB, Tet-on
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 17, 2006AvailabilityAcademic Institutions and Nonprofits only -
dCas9-KRAB-MeCP2
Plasmid#110821PurposeImproved dCas9 repressor-dCas9-KRAB-MeCP2DepositorInsertdCas9-KRAB-MeCP2
UseTagsExpressionMammalianMutationCas9 mutations to make dCas9=D10A+D839A+H840A+N86…PromoterCMVAvailable sinceJuly 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLX_311-KRAB-dCas9
Plasmid#96918Purposefor CRISPRi, lentiviral expression of KRAB-dCas9 and BlastR. Also called pXPR_121DepositorInsertdCas9
UseLentiviralTagsKRABExpressionMammalianMutationdCas9PromoterEF1aAvailable sinceJune 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-KRAS-G12D
Plasmid#116423PurposeLentiviral expression of KRAS G12DDepositorInsertKRAS (KRAS Human)
UseLentiviralTagsExpressionMutationG12DPromoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBabe-Kras G12D
Plasmid#58902PurposeExpresses human Kras with a G12D MutationDepositorInsertKRAS (KRAS Human)
UseRetroviralTagsHAExpressionMammalianMutationG12D mutationPromoterAvailable sinceSept. 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pX330a dCas9-KRAB
Plasmid#92361PurposeModified CAG promoter-containing vector for ubiquitous expression of catalytically inactive Cas9 fused to KRAB repressor domain. For targeted enhancer inactivation in chicken embryos.DepositorInsertdCas9-KRAB
UseCRISPRTagsExpressionMammalianMutationPromoterCAGAvailable sinceAug. 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLVCT-tTR-KRAB
Plasmid#11643PurposeTet-regulated (Tet-on) lentiviral vector for transgene (CAG promoter) - OR - shRNA (H1 promoter when subcloned from pLVTHM (Addgene#12247)) - 2nd generationDepositorInsertCAG promoters, GFP, tTR-KRAB, Tet-on
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 17, 2006AvailabilityAcademic Institutions and Nonprofits only -
SID4x-dCas9-KRAB
Plasmid#106399PurposeExpresses catalytically inactive dCas9 with N-terminal fusion of Sin3a Interacting Domain (SID) and C-terminal fusion of repressive KRAB domainDepositorUseTagsFLAG, HA, and NLSExpressionMammalianMutationPromoterAvailable sinceApril 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBabe-Kras G12C
Plasmid#58901PurposeExpresses human Kras with a G12C mutationDepositorInsertKRAS (KRAS Human)
UseRetroviralTagsHAExpressionMammalianMutationG12C mutationPromoterAvailable sinceSept. 15, 2014AvailabilityAcademic Institutions and Nonprofits only