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Plasmid#161763PurposepTRANS T-DNA vector with nptII selection and 35S:Cas9 with 6x Csy4-spliced gRNAs and rolD:TREX2 targeting the four MsPho2-1abcd and MsPho2-2abcd genes in alfalfa (pTRANS_220 - #91113)DepositorInsertCas9 and gRNAs
UseTagsExpressionPlantMutationPromoterCmYLCV PromoterAvailable sinceMay 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTRANS-Pho2-1/2-2-tRNA
Plasmid#161762PurposepTRANS T-DNA vector with nptII selection and 35S:Cas9 with 6x tRNA-spliced gRNAs and rolD:TREX2 targeting the four MsPho2-1abcd and MsPho2-2abcd genes in alfalfa (pTRANS_220 - #91113)DepositorInsertCas9 and gRNAs
UseTagsExpressionPlantMutationPromoterCmYLCV PromoterAvailable sinceMay 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMOD_B-Pho2-1/2-2-tRNA
Plasmid#161761PurposeMODULE B tRNA vector with 6x gRNAs targeting the four MsPho2-1abcd and MsPho2-2abcd genes in alfalfa (pMOD_B2303 - #91068)DepositorInsertgRNAs
UseTagsExpressionBacterialMutationPromoterCmYLCV PromoterAvailable sinceMay 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMOD_B-Pho2-1/2-2-Csy4
Plasmid#161760PurposeMODULE B Csy4 vector with 6x gRNAs targeting the four MsPho2-1abcd and MsPho2-2abcd genes in alfalfa (pMOD_B2103 - #91061)DepositorInsertgRNAs
UseTagsExpressionBacterialMutationPromoterCmYLCV PromoterAvailable sinceMay 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMpGE010-Target1 (Mpphot)
Plasmid#186728PurposeBinary vector for CRISPR/Cas9 (target 1: Mpphot [positive control]) in plants (for Agrobacterium-mediated genetic transformation).DepositorInsertMphot
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMpGE010-Target2 (Mpphot)
Plasmid#186729PurposeBinary vector for CRISPR/Cas9 (target 2: Mpphot [negative control]) in plants (for Agrobacterium-mediated genetic transformation).DepositorInsertMphot
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMpGE_En03-sgRNA_Target2 (Mpphot)
Plasmid#186727PurposeGateway entry vector for sgRNA (target 2: Mpphot [negative control]). Transient expression of sgRNA (target 2: Mpphot) in plant cells.DepositorInsertsgRNA_Mphot
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMpGE_En03-sgRNA_Target1 (Mpphot)
Plasmid#186726PurposeGateway entry vector for sgRNA (target 1: Mpphot [positive control]). Transient expression of sgRNA (target 1: Mpphot) in plant cells.DepositorInsertsgRNA_Mphot
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHCKAn-phoBp-GRFT
Plasmid#158746Purposelow phosphate phoBp induction of GriffithsinDepositorInsertphoBp Griffithsin
UseTagsExpressionBacterialMutationPromoterphoBAvailable sinceFeb. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAGM9121_Sce-Acid Phosphatase SP
Plasmid#153494PurposeS. cerevisiae Acid Phosphatase Signal Peptide Donor PlasmidDepositorInsertAcid Phosphatase SP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHCKan-phoEp-GFPuv
Plasmid#139074PurposeLow phosphate inducible promoter phoEp for GFP expressionDepositorInsertphoEp-GFPuv
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHCKan-phoHp1-GFPuv
Plasmid#139075PurposeLow phosphate inducible promoter phoHp1 for GFP expressionDepositorInsertphoHp-GFPuv
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHCKan-phoUp-GFPuv
Plasmid#139076PurposeLow phosphate inducible promoter phoUp for GFP expressionDepositorInsertphoUp-GFPuv
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLenti-Synapsin-pHoenix-WPRE
Plasmid#70111Purposelentiviral expression of pHoenix (Synaptophysin-pHluorin-Arch3-mKate2) driven by human Synapsin I promoter for optogenetic acidification of synaptic vesiclesDepositorInsertpHoenix (Syp Rat, Halorubrum sodomense, Entacmaea quadricolor, Aequorea victoria)
UseLentiviralTagsArch3, H+/K+ ATPase beta-subunit, mKate2, and pHl…ExpressionMutationPromoterSynapsinAvailable sinceNov. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-NES-PhoCl2f-mCherry
Plasmid#164048PurposeOptogenetic control of protein localization by PhoCl2fDepositorInsertNES-PhoCl2f-mCherry
UseTagskozak sequenceExpressionMammalianMutationPromoterCMV promotorAvailable sinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-NES-PhoCl-mCherry
Plasmid#87690PurposeExpress NES-PhoCl-mCherry in mammalian cellsDepositorInsertNES-PhoCl-mCherry
UseTagsnuclear export signalExpressionMammalianMutationPromoterCMVAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRK5-mEGFP-PHOX2B-MUT
Plasmid#194577PurposeMammalian Expression of mEGFP-PHOX2B Mutant (S207Afs*102) Fusion ProteinDepositorInsertPHOX2B (PHOX2B Human)
UseTagsmEGFPExpressionMammalianMutationS207Afs*102PromoterAvailable sinceFeb. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pfa6a PhoCl-TsCC(B)::KANMX
Plasmid#185906PurposePlasmid for cloning PhoCl fused to TsCC(B) into yeast.DepositorInsertPhoCl-TsCC(B)
UseTagsPhoCl fluorophore with C-terminal TsCC(B)ExpressionYeastMutationPromoterSP6Available sinceJuly 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-NBid-PhoCl2c-CBid
Plasmid#164051PurposeOptogenetic manipulation of cell apoptosis using PhoCl2cDepositorInsertNBid-PhoCl2c-CBid
UseTagskozak sequenceExpressionMammalianMutationPromoterCMV promotorAvailable sinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcNDA-NES-PhoCl2c-mCherry
Plasmid#164037PurposeOptogenetic control of protein localization by PhoCl2cDepositorInsertNES-PhoCl2c-mCherry
UseTagskozak sequenceExpressionMammalianMutationPromoterCMV promotorAvailable sinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only