We narrowed to 165,694 results for: addgene

1. pAAV-CMV-FLEX-SaCas9-U6-sgOprk1

   Plasmid

   #159903

   Purpose

   Mutagenesis of Oprk1

   Depositor

   Larry Zweifel

   Insert

   Oprk1 (Oprk1 Mouse)
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Available Since

   Nov. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAG-BE4max-P2A-EGFP (pRZ33)

   Plasmid

   #140083

   Purpose

   CAG promoter expression plasmid for human codon optimized BE4max C-to-T base editor with P2A-EGFP

   Depositor

   Keith Joung

   Insert

   Human codon optimized pCAG BE4max with P2A-EGFP
   

   Tags

   P2A-EGFP

   Expression

   Mammalian

   Mutation

   D10A (SpCas9)

   Promoter

   CAG

   Available Since

   March 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. XLone-Puro SPI1 P2A mCherry

   Plasmid

   #140029

   Purpose

   Tunable and temporal expression control of SPI1 and mCherry

   Depositor

   Xiaoping Bao

   Insert

   SPI1 (SPI1 Human)
   

   Use

   Synthetic Biology

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   TRE3G

   Available Since

   May 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1-HA_human_BAP1

   Plasmid

   #158109

   Purpose

   Expresses tagged BAP1, neomycin selection

   Depositor

   Emanuela Felley-Bosco

   Insert

   BAP1 (BAP1 Human)
   

   Expression

   Mammalian

   Available Since

   Oct. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. AAVS1_Puro_hPGK1_hGEM(1-110)_mNG_hSLBP(18-108)

   Plasmid

   #176932

   Purpose

   Donor for targeted integration of S-phase specific probe (FUCCI-S) to the AAVS1 safe harbor locus

   Depositor

   Yannick Doyon

   Insert

   hGEM(1-110)_mNG_hSLBP(18-108)
   

   Use

   Synthetic Biology

   Expression

   Mammalian

   Promoter

   PGK1

   Available Since

   Feb. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. MSCV-mMDM2-IRES-GFP

   Plasmid

   #66954

   Purpose

   retroviral construct for expression of mouse Mdm2 cloned into the EcoRI site

   Depositor

   Martine Roussel

   Insert

   Mdm2 (Mdm2 Mouse)
   

   Use

   Retroviral

   Tags

   IRES-GFP

   Expression

   Mammalian

   Promoter

   MSCV

   Available Since

   July 23, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. XLone-BSD SPI1 P2A mCherry

   Plasmid

   #140031

   Purpose

   Tunable and temporal expression control of SPI1 and mCherry

   Depositor

   Xiaoping Bao

   Insert

   SPI1 (SPI1 Human)
   

   Use

   Synthetic Biology

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   TRE3G

   Available Since

   May 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-CMV-FLEX-SaCas9-U6-sgNts

   Plasmid

   #159909

   Purpose

   Mutagenesis of Nts

   Depositor

   Larry Zweifel

   Insert

   Nts (Nts Mouse)
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Available Since

   Nov. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTF277 (pFA6a-TEV-6xGly-2xStrep-KanMX)

   Plasmid

   #44094

   Depositor

   Tim Formosa

   Type

   Empty backbone

   Use

   Pcr-based yeast c-terminal tagging

   Tags

   2xStrep tag, 6xGly linker, ADH1 transcription ter…

   Available Since

   April 10, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-CMV-FLEX-SaCas9-U6-sgVamp2

    Plasmid

    #159920

    Purpose

    Mutagenesis of Vamp2

    Depositor

    Larry Zweifel

    Insert

    Vamp2 (Vamp2 Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Available Since

    Nov. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. 4QQD

    Plasmid

    #61947

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    UHRF1:SDC130-H02:C39674 (UHRF1 Human)
    

    Tags

    6xHis and MHHHHHHSSGRENLYFQG

    Expression

    Bacterial

    Mutation

    See comments

    Promoter

    T7

    Available Since

    March 2, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

12. pLBS.EF/mScarlet(i)-hCdt100Nt

    Plasmid

    #129334

    Purpose

    Cell cycle reporter, lentivirus

    Depositor

    Yasushi Okada

    Article

    Yaginuma et al bioRxiv 2021.10.08.463131

    Insert

    mScarlet(i) (Addgene 85044)
    

    Use

    Lentiviral

    Available Since

    Oct. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTF279 (pFA6a-TEV-6xGly-2xStrep-HphMX)

    Plasmid

    #44082

    Depositor

    Tim Formosa

    Type

    Empty backbone

    Use

    Pcr-based yeast c-terminal tagging

    Tags

    2xStrep tag, 6xGly linker, ADH1 transcription ter…

    Available Since

    April 3, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. TPH2

    Plasmid

    #73243

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    TPH2 (TPH2 Human)
    

    Tags

    His-6-TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. TBC1D7

    Plasmid

    #32047

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    TBC1D7 (TBC1D7 Human)
    

    Tags

    His (with TEV cleavage)

    Expression

    Bacterial

    Available Since

    Jan. 3, 2012

    Availability

    Industry, Academic Institutions, and Nonprofits

16. 5VBD (USP09X)

    Plasmid

    #101260

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    USP09X (USP9X Human)
    

    Tags

    6xHis-TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 2, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. 8VIS

    Plasmid

    #220881

    Purpose

    Baculovirus expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    TMPRSS11D (TMPRSS11D Human)
    

    Use

    Baculovirus expresssion

    Tags

    EFVEHHHHHHHH

    Promoter

    Polyhedrin

    Available Since

    Sept. 30, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. pZC13 (pFA6a-TEV-6xGly-V5-HphMX)

    Plasmid

    #44085

    Depositor

    Tim Formosa

    Type

    Empty backbone

    Use

    Pcr-based yeast c-terminal tagging

    Tags

    6xGly linker, ADH1 transcription terminator, HphM…

    Available Since

    April 2, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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19. PANK1

    Plasmid

    #32871

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    PANK1 (PANK1 Human)
    

    Tags

    His tag (with Thrombin cleavage)

    Expression

    Bacterial

    Mutation

    amino acids 231-597 only

    Available Since

    Jan. 6, 2012

    Availability

    Industry, Academic Institutions, and Nonprofits

20. pUltra-hot-H2B-mTagBFP2

    Plasmid

    #161792

    Purpose

    3rd generation lentiviral multicistronic vector for mammalian expression of mCherry, H2B-mTagBFP2, and one gene of interest

    Depositor

    Jorge Luis Arias-Arias

    Insert

    H2B-mTagBFP2
    

    Use

    Lentiviral; Multicistronic

    Tags

    mCherry and mTagBFP2

    Expression

    Mammalian

    Mutation

    I174A in the mTagBFP2 protein according to PLoS O…

    Promoter

    UbC

    Available Since

    Nov. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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