We narrowed to 5,135 results for: codon optimized

1. pUbi_dCas9-BFP-KRAB-gRNA

   Plasmid

   #221503

   Purpose

   CRISPRi machinery fish codon optimized expressing plasmid

   Depositor

   Alessandro Ori

   Insert

   dCas9-tagBFP-KRAB
   

   Use

   Fish expression

   Mutation

   D10A and H839A point mutations

   Promoter

   Zebrafish Ubiquitin promoter

   Available Since

   July 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pHN872

   Plasmid

   #178830

   Purpose

   Bacterial expression for human Saposin A

   Depositor

   Kazuhiro Abe

   Insert

   artificial gene encoding human Saposin A
   

   Tags

   GST, His8, TEV

   Expression

   Bacterial

   Mutation

   Codon-usage was optimized for bacterial expressio…

   Promoter

   T7

   Available Since

   Jan. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pLenti-ABE7.10-P2A-Puro

   Plasmid

   #112674

   Purpose

   Lentivirus for constitutive expression of ABE7.10 (not codon optimized)

   Depositor

   Lukas Dow

   Insert

   ABE7.10
   

   Use

   Lentiviral

   Mutation

   D10A

   Promoter

   EF1s

   Available Since

   July 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pET15b-ACP (from S. aureus)

   Plasmid

   #63686

   Purpose

   Inducible expression of Acyl Carrier Protein (ACP) from S. aureus

   Depositor

   Michael Johnson

   Insert

   ACP
   

   Tags

   His

   Expression

   Bacterial

   Mutation

   codon optimized

   Promoter

   T7

   Available Since

   April 10, 2015

   Availability

   Academic Institutions and Nonprofits only

5. pFA6a-pTagRFP-T-kanMX6

   Plasmid

   #240555

   Purpose

   Tag S. pombe genes at 3' end (C-terminal end of protein) with TagRFP-T, codon-optimized for S. pombe

   Depositor

   Silke Hauf

   Insert

   pTagRFP-T
   

   Expression

   Yeast

   Available Since

   July 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pMG005_mNeonGreen_P2A

   Plasmid

   #237215

   Purpose

   Fluorescent reporter codon optimized for Dictyostelid species

   Depositor

   Jordi van Gestel

   Insert

   mNeonGreen-P2A
   

   Use

   Cloning

   Tags

   3xFLAG-tag, HA-tag, SV40-NLS, and nuleoplasmin NL…

   Available Since

   May 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pMG008_mCherry_P2A

   Plasmid

   #237216

   Purpose

   Fluorescent reporter codon optimized for Dictyostelid species

   Depositor

   Jordi van Gestel

   Insert

   mCherry-P2A
   

   Use

   Cloning

   Tags

   3xFLAG-tag, HA-tag, SV40-NLS, and nuleoplasmin NL…

   Available Since

   May 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCMV-T7-ABEmax(7.10)-SpRY-P2A-EGFP (RTW5025)

   Plasmid

   #140003

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized ABEmax(7.10) A-to-G base editor with SpRY(D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R) and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Walton et al Science. 2020 Apr 17;368(6488):290-296.

   Insert

   human codon optimized ABEmax(7.10) SpCas9 variant named SpRY with P2A-EGFP
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   P2A-EGFP

   Expression

   Mammalian

   Mutation

   nSpCas9=D10A; SpRY=A61R/L1111R/D1135L/S1136W/G121…

   Promoter

   CMV and T7

   Available Since

   March 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCMV-T7-SpRY-P2A-EGFP (RTW4830)

   Plasmid

   #139989

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized SpRY(A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Walton et al Science. 2020 Apr 17;368(6488):290-296.

   Insert

   human codon optimized SpCas9 variant named SpRY with BPNLS-3xFLAG-P2A-EGFP
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS-3xFLAG-P2A-EGFP

   Expression

   Mammalian

   Mutation

   SpRY=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N131…

   Promoter

   CMV and T7

   Available Since

   March 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pHAGE2-EF1a-E1MLS-2xMyc-Ku-IRES-Hygro

    Plasmid

    #234950

    Purpose

    Human codon optimized Ku (Mycobacterium) with N-terminal E1 MLS expressing plasmid

    Depositor

    Agnel Sfeir

    Article

    Fu et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

    Insert

    human codon optimized Ku with N-terminal mitochondrial localization signal of pyruvate dehydrogenase E1 subunit
    

    Use

    Lentiviral

    Tags

    Myc

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    April 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pHAGE2-EF1a-E1MLS-2xFLAG-LigD-IRES-tdTomato

    Plasmid

    #234951

    Purpose

    Human codon optimized LigD (Mycobacterium) with N-terminal E1 MLS expressing plasmid

    Depositor

    Agnel Sfeir

    Article

    Fu et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

    Insert

    Human codon optimized LigD with N-terminal mitochondrial localization signal of pyruvate dehydrogenase E1 subunit
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    EF1A

    Available Since

    April 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCMV-T7-SpRY-HF1-P2A-EGFP (RTW5008)

    Plasmid

    #139997

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized SpRY-HF1(SpRY=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R;HF1=N497A/R661A/Q695A/Q926A) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Walton et al Science. 2020 Apr 17;368(6488):290-296.

    Insert

    human codon optimized SpCas9 variant named SpRY-HF1 with BPNLS-3xFLAG-P2A-EGFP
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    SpRY=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N131…

    Promoter

    CMV and T7

    Available Since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pUCIDT-attL1-Worm ABeta-attR5

    Plasmid

    #160437

    Purpose

    Entry vector for cloning nematode-codon-optimized Amyloid Beta(1-42) using 2-fragment gateway recombination

    Depositor

    Nicholas Tolwinski

    Article

    Kaur et al Bio Protoc. 2020 Dec 5;10(23):e3856. doi

    Insert

    Homo sapiens amyloid beta precursor protein (APP Nematode, Human)
    

    Tags

    Cleavage signal

    Expression

    Bacterial

    Mutation

    Codon-optimized for expression in C. elegans

    Available Since

    Oct. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCMV-T7-SpG-HF1-P2A-EGFP (RTW5000)

    Plasmid

    #139996

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized SpG-HF1(SpG=D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R;HF1=N497A/R661A/Q695A/Q926A) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Walton et al Science. 2020 Apr 17;368(6488):290-296.

    Insert

    human codon optimized SpCas9 variant named SpG-HF1 with BPNLS-3xFLAG-P2A-EGFP
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    SpG=D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R; HF…

    Promoter

    CMV and T7

    Available Since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pBOB-CAG-SARS-CoV2-Spike-HA

    Plasmid

    #141347

    Purpose

    3rd Generation lentiviral vector expressing the codon-optimized SARS-CoV2 Spike Glycoprotein ORF with a C-terminal HA tag generated by Junko Ogawa & Gerald M Pao

    Depositor

    Gerald Pao

    Article

    Ogawa et al bioRxiv. 2020 Jul 22. doi: 10.1101/2020.

    Insert

    SARS-CoV2 Spike Glycoprotein (S SARS-CoV2 hCoV19_USA EPI_ISL_414366 (GISAID))
    

    Use

    Lentiviral

    Tags

    HA

    Mutation

    resynthesized with human codon optimization nucle…

    Promoter

    CAG

    Available Since

    May 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pNMSB104

    Plasmid

    #199314

    Purpose

    MosSCI insertion of flp-18p::lox2272::mTagBFP2::tbb-2 3'UTR::lox2272::ChRmine::SL2::jRGECO1a::let-858 3'UTR at ttTi5605

    Depositor

    Michael Krieg

    Article

    Porta-de-la-Riva et al Nat Methods. 2023 Apr 6. doi: 10.1038/s4

    Insert

    flp-18p::lox2272::mTagBFP2::tbb-2 3'UTR::lox2272::ChRmine::SL2::jRGECO1a::let-858 3'UTR
    

    Expression

    Worm

    Mutation

    mTagBFP2 from pJJR81, C. elegans codon optimized …

    Promoter

    flp-18

    Available Since

    Aug. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. LeGO-iBSD-5LO-WT

    Plasmid

    #182741

    Purpose

    Lentiviral expression vector for codon-optimized 5-Lipoxygenase (WT) in mamallian cells with blasticidin S resistance (bsd).

    Depositor

    Dieter Steinhilber

    Article

    Kreiss et al Biochem Pharmacol. 2022 Jul 22:115187. d

    Insert

    5-Lipoxygenase (ALOX5 Human)
    

    Use

    Cre/Lox and Lentiviral

    Expression

    Mammalian

    Mutation

    codon optimized for expression in homo sapiens

    Promoter

    SFFV

    Available Since

    July 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pHL-EF1a-SphcCas9-iP-A

    Plasmid

    #60599

    Purpose

    Expresses human codon-optimized Cas9 (derived from Streptococcus pyogenes) and pruomycin resistance gene.

    Depositor

    Akitsu Hotta

    Article

    Li et al Stem Cell Reports. 2014 Nov 25. pii: S22

    Insert

    CRISPR Cas9
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    Codon usage optimized for human usage

    Available Since

    Dec. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pSH-EFIRES-P-GFP(1-10)opti

    Plasmid

    #129416

    Purpose

    Expressing codon-optimized GFP(1-10) fragment in human cells

    Depositor

    Elina Ikonen

    Article

    Salo et al Dev Cell. 2019 Jun 6. pii: S1534-5807(19

    Insert

    codon-optimized GFP(1-10)
    

    Use

    CRISPR, TALEN, and Unspecified ; Human safe harbo…

    Expression

    Mammalian

    Promoter

    EF-1α

    Available Since

    Aug. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA5-FRT-TO-Nprot-HF_GC3opt

    Plasmid

    #157732

    Purpose

    mammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 N protein under control of a tetracycline-inducible promoter

    Depositor

    David Tollervey

    Article

    Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

    Insert

    Nprot-HF_GC3opt (N SARS-CoV-2)
    

    Tags

    His8-Flag

    Expression

    Mammalian

    Mutation

    codon optimized; gene expression was optimized by…

    Available Since

    Aug. 10, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge