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pdCas9 (GB1079)
(Plasmid #75399)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 75399 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pUPD
  • Backbone manufacturer
    self-made; derived from pGEMT-Easy manufactured by Promega
  • Backbone size w/o insert (bp) 3055
  • Total vector size (bp) 7192
  • Vector type
    Plant Expression, CRISPR, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Cas9 coding region with mutated (D10A, H840A) and inactivated catalytic domains (human codon optimised)
  • Species
    Streptococcus pyogenes
  • Insert Size (bp)
    4137

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BsmBI (destroyed during cloning)
  • 3′ cloning site BsmBI (destroyed during cloning)
  • 5′ sequencing primer T7
  • 3′ sequencing primer SP6
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pdCas9 (GB1079) was a gift from Diego Orzaez (Addgene plasmid # 75399 ; http://n2t.net/addgene:75399 ; RRID:Addgene_75399)
  • For your References section:

    A modular toolbox for gRNA-Cas9 genome engineering in plants based on the GoldenBraid standard. Vazquez-Vilar M, Bernabe-Orts JM, Fernandez-Del-Carmen A, Ziarsolo P, Blanca J, Granell A, Orzaez D. Plant Methods. 2016 Feb 1;12:10. doi: 10.1186/s13007-016-0101-2. eCollection 2016. 101 [pii] PubMed 26839579