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pHH0103_MAP3K9-1/2

Plasmid

#91313

Purpose

Protein expression and purification of human SH3 domain construct MAP3K9-1/2

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

MAP3K9-1/2 (MAP3K9 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_ARHGAP12-1/1

Plasmid

#91282

Purpose

Protein expression and purification of human SH3 domain construct ARHGAP12-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

ARHGAP12-1/1 (ARHGAP12 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_AHI1-1/1

Plasmid

#91269

Purpose

Protein expression and purification of human SH3 domain construct AHI1-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

AHI1-1/1 (AHI1 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_ARHGEF9-1/1

Plasmid

#91417

Purpose

Protein expression and purification of human SH3 domain construct ARHGEF9-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

ARHGEF9-1/1 (ARHGEF9 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pet21a-aGFPnb-enhancer-cys-linker-YbbR-(PAS)5-H6

Plasmid

#192788

Purpose

aGFP nanobody enhancer tagged with cysteine, YbbR and H6 for bacterial expression, purification and labeling

Depositor

Jacob Piehler

Article

Sotolongo Bellon et al Cell Rep Methods. 2022 Feb 4;2(2):100165

Insert

antiGFP nanobody enhancer

Tags

H6 and ybbR

Expression

Bacterial

Available Since

Dec. 21, 2022

Availability

Academic Institutions and Nonprofits only

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HOXB3 (human) FLAG pSP65

Plasmid

#8523

Depositor

Corey Largman

Article

Shen et al Nucleic Acids Res 1996 Mar 1;24(5):898-9

Insert

HOXB3 (HOXB3 Human)

Use

In vitro transcription/translation

Tags

FLAG

Expression

Bacterial

Mutation

Contains 5 additional amino acids before ATG from…

Available Since

May 30, 2006

Availability

Academic Institutions and Nonprofits only
pEGB 35S:dCas9:Tnos (GB1191)

Plasmid

#68223

Purpose

Transcriptional unit for human codon optimized with mutated (D10A, H840A) and inactivated catalytic domains Cas9 protein plant expression driven by the 35S promoter

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

dCas9

Use

CRISPR and Synthetic Biology

Expression

Plant

Mutation

BsaI and BsmBI sites removed; human codon optimis…

Promoter

35S

Available Since

March 16, 2016

Availability

Academic Institutions and Nonprofits only

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[UBC][EIF4e][GG cloning]

Plasmid

#97189

Purpose

EIF4e plasmid with Pum cloning site

Depositor

Edward Boyden

Article

Adamala et al Proc Natl Acad Sci U S A. 2016 May 10;11

Insert

EIF4E variant 1 (EIF4E Human)

Use

Synthetic Biology

Tags

empty Pum cloning site

Expression

Mammalian

Promoter

UBC (ubiquitin C)

Available Since

Nov. 2, 2017

Availability

Academic Institutions and Nonprofits only

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pCAG-AviMta3-3xFiBsd

Plasmid

#140962

Purpose

full-length wt Avi-Mta3-3XFLAG-iBsd cDNA w/CAG promoter and Blasticidin resistance, cloned from mouse ES cells

Depositor

Brian Hendrich

Article

Burgold et al EMBO J. 2019 Jun 17;38(12). pii: embj.20

Insert

metastasis associated 3 (Mta3 Mouse, Synthetic)

Tags

3xFLAG, Avi, and TEV

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pLNHA-C1-HsNot9_18-285_S

Plasmid

#147480

Purpose

Mammalian Expression of HsNot9_18-285

Depositor

Elisa Izaurralde

Article

Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

Insert

HsNot9_18-285 (CNOT9 Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsNot9_18-285_S

Plasmid

#147482

Purpose

Mammalian Expression of HsNot9_18-285

Depositor

Elisa Izaurralde

Article

Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

Insert

HsNot9_18-285 (CNOT9 Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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Nsp14D-mCherry

Plasmid

#165139

Purpose

mammalian expression and localization

Depositor

Bruno Antonny

Article

Miserey-Lenkei et al Biol Cell. 2021 Jul;113(7):311-328. doi:

Insert

Nsp14D (ORF1ab codon optimized SARS-COV-2)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

March 2, 2021

Availability

Academic Institutions and Nonprofits only

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Nsp4 deltaC-EGFP

Plasmid

#165126

Purpose

mammalian expression and localization

Depositor

Bruno Antonny

Article

Miserey-Lenkei et al Biol Cell. 2021 Jul;113(7):311-328. doi:

Insert

Nsp4 deltaC (ORF1ab codon optimized SARS-COV-2)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pGEX-6P-1-INTS3-C

Plasmid

#128417

Purpose

Express INTS3 C-termini (514-1042 aa) in E. coli with a GST tag (N-terminal)

Depositor

Yuliang Wu

Article

Vidhyasagar et al Biochem J. 2018 Jan 2;475(1):45-60. doi:

Insert

INTS3 (INTS3 Human)

Tags

GST

Expression

Bacterial

Mutation

INTS3 C-terminal region (514-1042 aa)

Promoter

Tac promoter

Available Since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV6-XL4 ASXL1 (p.R404X) 3x FLAG

Plasmid

#74263

Purpose

Cancer-associated ASXL1 truncation mutant in mammalian expression vector

Depositor

Anjana Rao

Article

Balasubramani et al Nat Commun. 2015 Jun 22;6:7307. doi: 10.

Insert

ASXL1 (ASXL1 Human)

Tags

3X FLAG

Expression

Mammalian

Mutation

p.R404X truncation

Promoter

CMV

Available Since

Dec. 21, 2016

Availability

Academic Institutions and Nonprofits only
pDEST27-GST-ATXN1 FL(92Q)-S776D

Plasmid

#21757

Depositor

Huda Zoghbi

Article

Lim et al Nature. 2008 Apr 10. 452(7188):713-8.

Insert

Ataxin-1 (ATXN1 Human)

Tags

GST

Expression

Mammalian

Mutation

insert contains a stretch of 92 Q(Glutamines) and…

Promoter

CMV

Available Since

July 22, 2014

Availability

Academic Institutions and Nonprofits only
pEBTetDBl-CLIP-α-tubulin

Plasmid

#136841

Purpose

Mammalian expression of the centrosomal protein _-tubulin N-terminally fused to CLIP-tag

Depositor

Kai Johnsson

Article

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Insert

CLIP-TUBA (DNMBP Human, Synthetic)

Tags

FLAG-tag / His-tag

Expression

Mammalian

Promoter

CMV-TetO2

Available Since

March 31, 2020

Availability

Academic Institutions and Nonprofits only

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pHH0103_ARHGEF6-1/1

Plasmid

#91515

Purpose

Protein expression and purification of human SH3 domain construct ARHGEF6-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

ARHGEF6-1/1 (ARHGEF6 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pEBTet-SNAP-Cep70

Plasmid

#136821

Purpose

Mammalian expression of the centrosomal protein Cep70 N-terminally fused to SNAP-tag

Depositor

Kai Johnsson

Article

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Insert

SNAP-Cep70 (CEP70 Human, Synthetic)

Tags

FLAG-tag / His-tag

Expression

Mammalian

Promoter

CMV-TetO2

Available Since

March 26, 2020

Availability

Academic Institutions and Nonprofits only

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GFP-AuroraB L154G/H250Y

Plasmid

#108492

Purpose

expression of EGFP-AuroraB Analog sensitive (L154G/H250Y)

Depositor

Susanne Lens

Article

Hengeveld et al Mol Cell Proteomics. 2012 May;11(5):47-5

Insert

AuroraB (AURKB Human)

Tags

EGFP

Expression

Mammalian

Mutation

Analog sensitive L154G/H250Y

Promoter

CMV

Available Since

May 8, 2018

Availability

Academic Institutions and Nonprofits only

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