We narrowed to 26,182 results for: Cre
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Plasmid#175363PurposeZeoR overexpression of cytosolic GFP in Drosophila ExpreS2ion cellsDepositorInsertsgGFP
ExpressionInsectMutationF64L, S65C, and I167TPromoterFused Actin-HSP70Available SinceMarch 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV_Anc689-NG-nCas9
Plasmid#163527PurposeMammalian CG-to-GC base editingDepositorInsertAnc689-NG-nCas9
UseCRISPRExpressionMammalianMutationnCas9 (D10A)NG (L111R, D1135V, G1218R, E1219F, A1…Available SinceDec. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMTU-DO-HPH
Plasmid#160008PurposeLow-copy expression vector for K. marxianus, hygromycin selectionDepositorTypeEmpty backboneUseKluyveromyces marxianusExpressionYeastPromotern/aAvailable SinceNov. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-SARS2-S-G614 (NC-flag)
Plasmid#156419PurposeMammalian expression of SARS-CoV-2 S protein (D614) with the flag tag at N- and C-termini. Pseudotypes onto the MLV vector but not efficiently to VSV or lentiviral vectors.DepositorInsertSARS-CoV-2 S protein (S SARS CoV2)
TagsFlagExpressionMammalianMutationD614GPromoterCMVAvailable SinceAug. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pOCC72
Plasmid#118866Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6 tag, cleavable with 3C, and N-terminal monomeric GFP, cleavable with TEVDepositorInsertNcoI-HIS6-3C-mGFP-TEV-NotI-ccdB-AscI-stop-HindIII cassette
TagsHIS6, cleavable with 3C protease, mGFP, cleavable…ExpressionInsectPromoterpolHAvailable SinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
MSCV-Neo-GFP/Flag-Gata5
Plasmid#105508PurposeMSCV-driven retroviral Gata5 expression (Flag-tagged)DepositorAvailable SinceMay 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
Ii-Str_ManII-SBP-tagBFP
Plasmid#65257Purposesynchronize trafficking of ManII from the ER (RUSH system)DepositorInsertIi-Streptavidin and truncated Mannosidase II
TagsStreptavidin Binding Protein (SBP) and tagBFPExpressionMammalianMutationcontains only amino acids 1 to 116 of ManIIPromoterCMVAvailable SinceJune 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
Cenp-B INCENP mCherry
Plasmid#45235PurposeTarget INCENP to centromeres via full length CENP-BDepositorInsertCenp-B, INCENP-Δcen, mCherry
Tagsfull length CENP-B and mCherryExpressionMammalianMutationCEN box deleted from INCENP (aa1-46)Available SinceSept. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSox2-bd::FP
Plasmid#34703DepositorInserteGFP
UseXenopus expressionTags165-bp minimal FGF4 promoter, 6x Sox2/Oct3-4 tran…Available SinceFeb. 17, 2012AvailabilityAcademic Institutions and Nonprofits only -
pENTR1a-CRISPRoffv2.1
Plasmid#207174PurposeGateway Cloning Vector for CRISPRoffv2.1DepositorInsertCRISPRoffv2.1
UseCRISPRTagsTagBFPExpressionMammalianAvailable SinceApril 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
GCGR-DuET
Plasmid#213250PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorAvailable SinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
CHRM2-DuET
Plasmid#213208PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorAvailable SinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
ADORA2B-DuET
Plasmid#213175PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorAvailable SinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
ADORA1-DuET
Plasmid#213173PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorAvailable SinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCI pHluorin Syt7
Plasmid#184508PurposeMammalian expression of Synaptotagmin 7 with pH-sensitive fluorescent proteinDepositorAvailable SinceMay 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMV361_MjTyrRS
Plasmid#193250PurposeExpresses the M. jannaschii tyrosyl-tRNA synthetase (MjTyrRS)DepositorInsertMjTyrRS
ExpressionBacterialPromoterhsp60Available SinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pspCas9-Klenow-ST2-com-vector
Plasmid#176235PurposeVector plasmid for expressing spCas9-Klenow fusion protein and sgRNA. There are two copies of HBB 3’ UTR in the 3’UTR of spCas9-Klenow to enhance expression.DepositorTypeEmpty backboneExpressionBacterialAvailable SinceMarch 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMX-DivisionRecorder
Plasmid#179446PurposeRetroviral expression vector containing the DivisionRecorder cassetteDepositorInsertGFP-P2A-511-CrePI-511
UseRetroviralPromoterLTRAvailable SinceMarch 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
HB401: pMVP (L1-L4) CMV promoter
Plasmid#121686PurposepMVP L1-L4 entry plasmid, contains CMV promoter for 3-component MultiSite Gateway Pro assemblyDepositorInsertCMV promoter
UseGateway Cloning and Synthetic BiologyAvailable SinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
IF101: pMVP (L1-L4) EF1a promoter
Plasmid#121687PurposepMVP L1-L4 entry plasmid, contains EF1a promoter for 3-component MultiSite Gateway Pro assemblyDepositorInsertEF1a promoter
UseGateway Cloning and Synthetic BiologyAvailable SinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only