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pCDF_AaRaf1/Raf2/BSD2

Plasmid

#229515

Purpose

Expresses Anthoceros agrestis chaperones: Raf1,Raf2,BSD2

Depositor

Laura Gunn

Article

Oh et al Mol Plant. 2024 Dec 2;17(12):1833-1849.

Inserts

Rubisco accumulation factor 1
Rubisco accumulation factor 2
BSD2

Expression

Bacterial

Mutation

N terminus chloroplast transit peptide truncation

Promoter

T7

Available Since

June 20, 2025

Availability

Academic Institutions and Nonprofits only

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pALN132

Plasmid

#239799

Purpose

Express mEGFP tagged mouse CENPT chimeric protein containing the rat CENPT histone fold domain

Depositor

Iain Cheeseman, Ally Nguyen

Article

Dudka et al Curr Biol. 2025 Mar 10;35(5):1012-1022.e

Insert

MmCENPT--Rn-Histone Fold Domain

Use

Retroviral

Tags

mEGFP

Expression

Mammalian

Promoter

Moloney Murine Leukemia Virus (MMLV) LTR promoter

Available Since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

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pME_Cp_0_1_010_psbD_5'Hom_Cr

Plasmid

#235788

Purpose

5' Homology psbD in the M1 position used for genome integration at the psbD locus

Depositor

Tobias Erb

Article

Inckemann et al Nat Plants. 2025 Nov;11(11):2332-2349. d

Insert

5' Homology region of the psbD locus of Chlamydomonas reinhardtii chloroplast CC-125

Use

Synthetic Biology

Mutation

none

Available Since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

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pME_Cp_0_1_005_rbcL_5'Hom_Cr

Plasmid

#235786

Purpose

5' Homology rbcL in the M1 position used for genome integration at the rbcL locus

Depositor

Tobias Erb

Article

Inckemann et al Nat Plants. 2025 Nov;11(11):2332-2349. d

Insert

5' Homology region of the rbcL locus of Chlamydomonas reinhardtii chloroplast CC-125

Use

Synthetic Biology

Mutation

none

Available Since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

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pGEMHE-MmCENP-W

Plasmid

#237433

Purpose

Expresses Mus musculus CENP-W; untagged; made for in vitro transcription

Depositor

Michael Lampson

Article

Dudka et al Curr Biol. 2025 Mar 10;35(5):1012-1022.e

Insert

Mus musculus CENP-W (Cenpw Mouse)

Expression

Mammalian

Promoter

T7

Available Since

June 2, 2025

Availability

Academic Institutions and Nonprofits only

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pGEMHE-MmCENPT-GFP

Plasmid

#237426

Purpose

Expresses Mus musculus CENP-T tagged with GFP at C-term; made for in vitro transcription (T7 promoter)

Depositor

Michael Lampson

Article

Dudka et al Curr Biol. 2025 Mar 10;35(5):1012-1022.e

Insert

Mus musculus CENP-T (Cenpt Mouse)

Tags

GFP

Expression

Mammalian

Promoter

T7

Available Since

June 2, 2025

Availability

Academic Institutions and Nonprofits only

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BtA3CL

Plasmid

#238992

Purpose

In vitro transcription of mutant bovine arrestin-3 short isoform

Depositor

Vsevolod Gurevich

Article

Ahmed et al Biochemistry. 2011 May 10;50(18):3749-63

Insert

arrestin-3 short (ARR3 Bovine)

Use

In vitro transcription

Mutation

C17S, C60 V, C126S, C141L, C151V, C243V, C252V, C…

Promoter

SP6

Available Since

May 27, 2025

Availability

Academic Institutions and Nonprofits only

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pYO1124

Plasmid

#235725

Purpose

Expression of human UVRAG (1-464) fused to human ATG14L BATS (413-492) in mammalian cells

Depositor

Roger Williams

Article

Tremel et al Nat Commun. 2021 Mar 10;12(1):1564. doi:

Insert

UVRAG(1-464) fused to ATG14L BATS domain (413-492)

Expression

Mammalian

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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pJB180

Plasmid

#235737

Purpose

Expression of mCherry- human RAB1A Q70L in mammalian cells

Depositor

Roger Williams

Article

Tremel et al Nat Commun. 2021 Mar 10;12(1):1564. doi:

Insert

RAB1A (RAB1A Human)

Tags

mCherry

Expression

Mammalian

Mutation

Q70L

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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pYO1296

Plasmid

#235710

Purpose

Expression of mCherry-human RAB5A Q79L in mammalian cells

Depositor

Roger Williams

Article

Tremel et al Nat Commun. 2021 Mar 10;12(1):1564. doi:

Insert

RAB5 (RAB5A Human)

Tags

mCherry

Expression

Mammalian

Mutation

Q79L, aa 2-215

Available Since

May 13, 2025

Availability

Academic Institutions and Nonprofits only

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pICH47822_Myc-Sr62TK

Plasmid

#233536

Purpose

Expresses Myc-Sr62TK in planta

Depositor

Brande Wulff

Article

Chen et al bioRxiv 2024.08.30.610287

Insert

Sr62TK

Tags

Myc tag

Expression

Plant

Promoter

35S

Available Since

May 8, 2025

Availability

Academic Institutions and Nonprofits only

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pME_Cp_0_4_022_aac6-aph2-marker

Plasmid

#235898

Purpose

aac6-aph2 tobramycin resistance gene, codon optimized for Chlamydomonas reinhardtii

Depositor

Tobias Erb

Article

Inckemann et al Nat Plants. 2025 Nov;11(11):2332-2349. d

Insert

aac6-aph2-marker

Use

Synthetic Biology

Available Since

May 5, 2025

Availability

Academic Institutions and Nonprofits only

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pME_Cp_0_1_001_psbH_5'Hom_Cr

Plasmid

#235784

Purpose

5' Homology psbH in the M1 position used for genome integration at the psbH locus

Depositor

Tobias Erb

Article

Inckemann et al Nat Plants. 2025 Nov;11(11):2332-2349. d

Insert

5' Homology region of the psbH locus of Chlamydomonas reinhardtii chloroplast CC-125

Use

Synthetic Biology

Mutation

none

Available Since

May 5, 2025

Availability

Academic Institutions and Nonprofits only

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pME_Cp_0_1_004_psbA_5'Hom_Cr

Plasmid

#235785

Purpose

5' Homology psbA in the M1 position used for genome integration at the psbA locus

Depositor

Tobias Erb

Article

Inckemann et al Nat Plants. 2025 Nov;11(11):2332-2349. d

Insert

5' Homology region of the psbA locus of Chlamydomonas reinhardtii chloroplast CC-125

Use

Synthetic Biology

Mutation

none

Available Since

May 5, 2025

Availability

Academic Institutions and Nonprofits only

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pME_Cp_0_1_007_petB_5'Hom_cr

Plasmid

#235787

Purpose

5' Homology petB in the M1 position used for genome integration at the petB locus

Depositor

Tobias Erb

Article

Inckemann et al Nat Plants. 2025 Nov;11(11):2332-2349. d

Insert

5' Homology region of the petB locus of Chlamydomonas reinhardtii chloroplast CC-125

Use

Synthetic Biology

Mutation

none

Available Since

May 5, 2025

Availability

Academic Institutions and Nonprofits only

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pLIB-His(N)-SSU72

Plasmid

#231723

Purpose

Protein expression in insect cells

Depositor

Jesper Svejstrup

Article

Lopez Martinez et al Mol Cell. 2025 May 15;85(10):1952-1967.e

Insert

SSU72 (SSU72 Human)

Tags

6xHis-3C

Expression

Insect

Available Since

May 2, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV[FLEX]-CAG-EGFP-pri-miR-137

Plasmid

#235159

Purpose

Expresses EGFP and miR-137 primary transcript in a Cre recombinase-dependent manner

Depositor

Giordano Lippi

Article

Zolboot et al Neuron. 2025 Apr 1:S0896-6273(25)00181-3

Insert

EGFP/pri-miR-137 (Mir137 Mouse)

Use

AAV

Promoter

CAG

Available Since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-rev(CMV-TagBFP2)-EFS-mCherry-124-MUT-MRE

Plasmid

#235148

Purpose

Expresses the mCherry-based mutated (control) sensor for miR-124. TagBFP2 is expressed as a transduction reporter

Depositor

Giordano Lippi

Article

Zolboot et al Neuron. 2025 Apr 1:S0896-6273(25)00181-3

Insert

mCherry with a mutated miR-124 MRE

Use

AAV

Mutation

7 nucleotides of the miR-124 WT MRE mutated

Promoter

EFS

Available Since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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p3VEA3-3A

Plasmid

#234797

Purpose

Mammalian expression of bovine arrestin-3 (I385A,V386A,F387A)

Depositor

Vsevolod Gurevich

Article

Breitman et al J Biol Chem. 2012 Jun 1;287(23):19653-64

Insert

Arrestin-3 (ARR3 Bovine)

Tags

Venus

Expression

Mammalian

Mutation

I385A,V386A,F387A

Available Since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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pYO1262

Plasmid

#235727

Purpose

Protein expression of His6-RAB1A Q70L 1-203 in bacteria

Depositor

Roger Williams

Article

Tremel et al Nat Commun. 2021 Mar 10;12(1):1564. doi:

Insert

RAB1A (RAB1A Human)

Tags

6xHis-TEV

Expression

Bacterial

Mutation

Q70L, aa 1-203

Available Since

April 16, 2025

Availability

Academic Institutions and Nonprofits only

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