We narrowed to 9,669 results for: Coli

1. pKP2010

   Plasmid

   #167603

   Purpose

   Express ExbB (235-244 Ala),ExbD

   Depositor

   Kathleen Postle

   Insert

   ExbB, ExbD
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   exbB gene with ala codons replacing codons 235-24…

   Promoter

   Available Since

   July 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pKP2019

   Plasmid

   #167604

   Purpose

   Express ExbB (195-204 Ala),ExbD

   Depositor

   Kathleen Postle

   Insert

   ExbB, ExbD
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   exbB gene with ala codons replacing codons 195-20…

   Promoter

   Available Since

   July 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pKP2040

   Plasmid

   #167650

   Purpose

   Express His6-ExbB S80A, S82A, S84A

   Depositor

   Kathleen Postle

   Insert

   his6-exbB S80A, S82A, S84A
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   ExbB S80A, S82A, S84A with His6 tag at N terminus

   Promoter

   Available Since

   July 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pKP2025

   Plasmid

   #167605

   Purpose

   Express ExbB (225-234 Ala),ExbD

   Depositor

   Kathleen Postle

   Insert

   ExbB, ExbD
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   exbB gene with ala codons replacing codons 225-23…

   Promoter

   Available Since

   June 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pKP2008

   Plasmid

   #167601

   Purpose

   Express ExbB (205-214 Ala),ExbD

   Depositor

   Kathleen Postle

   Insert

   ExbB, ExbD
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   exbB gene with ala codons replacing codons 205-21…

   Promoter

   Available Since

   June 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCH1/RAD51o

   Plasmid

   #102562

   Purpose

   Express Human RAD51 protein codon optimzed for E.coli and the E.coli GorE Operon

   Depositor

   Maria Spies

   Article

   Subramanyam et al Proc Natl Acad Sci U S A. 2016 Oct 11;11

   Inserts

   Homo sapiens RAD51 homolog (RecA homolog, E. coli) (S. cerevisiae) (RAD51), transcript variant 1 (RAD51 Human, Synthetic)
   E. coli groE operon
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Codon optimized for E.coli Expression

   Promoter

   T7

   Available Since

   Feb. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pNeae2

   Plasmid

   #168300

   Purpose

   Bacterial surface display of polypeptides (nanobodies) in E. coli

   Depositor

   Luis Ángel Fernández

   Article

   Salema et al PLoS One. 2013 Sep 23;8(9):e75126. doi:

   Insert

   Enterohemorraghic E. coli Intimin N-terminal fragment, E-tag, 6xHis tag, and myc-tag at the C-terminus
   

   Use

   Tags

   6xHis, E-tag, and myc-tag

   Expression

   Bacterial

   Mutation

   Promoter

   LacI-Plac

   Available Since

   June 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET-TRSter

   Plasmid

   #78865

   Purpose

   Expresses rat Thioredoxin Reductase using an open reading frame in fusion with a mutated E. coli-type SECIS element

   Depositor

   Elias Arnér

   Article

   Arner et al J Mol Biol. 1999 Oct 8;292(5):1003-16.

   Insert

   Thioredoxin Reductase (Txnrd1 Rat)
   

   Use

   Tags

   Mutated E. coli-type SECIS element (3’ end of ins…

   Expression

   Bacterial

   Mutation

   Promoter

   T7lac

   Available Since

   Sept. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSEVA228-pro4IUPi

   Plasmid

   #122018

   Purpose

   Expresses IUP pathway genes (ChK, IPK, idi) under constitutive promoter. Kan. resist.

   Depositor

   Gregory Stephanopoulos

   Article

   Chatzivasileiou et al Proc Natl Acad Sci U S A. 2018 Dec 24. p

   Inserts

   Choline kinase (CKI1 Budding Yeast)
   Isopentenyl monophosphate kinase (AT1G26640 Mustard Weed)
   isopentenyl-diphosphate Delta-isomerase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Codon optimized for E. coli

   Promoter

   pro4

   Available Since

   Oct. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTrc99S-ssDsbA-MBP-4xDQNAT

    Plasmid

    #128398

    Purpose

    Periplasmic expression of E. coli MBP with C terminal 4xDQNAT glycosylation sites in E. coli

    Depositor

    Michael Jewett

    Article

    Stark et al Sci Adv. 2021 Feb 3;7(6). pii: 7/6/eabe9

    Insert

    Maltose binding protein
    

    Use

    Tags

    4xDQNAT glycosylation tag, 6xHis tag, and DsbA si…

    Expression

    Bacterial

    Mutation

    Y342H- please see depositor comments

    Promoter

    trc

    Available Since

    April 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-ITR-EcYtR-mCherry

    Plasmid

    #217362

    Purpose

    Expresses E. coli tyrosine tRNA and a wild-type mCherry reporter; can be packaged into AAV

    Depositor

    Abhishek Chatterjee

    Article

    Jewel et al Nat Methods. 2023 Jan;20(1):95-103. doi:

    Insert

    E. coli tyrosine tRNA
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    April 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCK301

    Plasmid

    #87767

    Purpose

    E. coli rhaBAD promoter upstream of sfGFP, sfGFP can be replaced with any gene of interest to express using L-rhamnose, three terminators, ampR, pBR322 origin, lacI

    Depositor

    John Heap

    Article

    Kelly et al ACS Synth Biol. 2016 Oct 21;5(10):1136-1

    Insert

    PrhaBAD-sfGFP
    

    Use

    Synthetic Biology

    Tags

    6xHis Tag

    Expression

    Bacterial

    Mutation

    Promoter

    PrhaBAD rhamnose-inducible promoter from E. coli

    Available Since

    May 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTET-IUPi

    Plasmid

    #122019

    Purpose

    Expresses IUP pathway genes (ChK, IPK, idi) under inducible promoter. aTC inducible. Kan. resist.

    Depositor

    Gregory Stephanopoulos

    Article

    Chatzivasileiou et al Proc Natl Acad Sci U S A. 2018 Dec 24. p

    Inserts

    Choline kinase (CKI1 Budding Yeast)
    Isopentenyl monophosphate kinase (AT1G26640 Mustard Weed)
    isopentenyl-diphosphate Delta-isomerase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Codon optimized for E. coli

    Promoter

    pTET

    Available Since

    Oct. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pYihI-N-term D-N/E-Q

    Plasmid

    #233095

    Purpose

    Expression of GST-YihI with N-term aspartic acids (D) mutated to asparagine (N) and glutamic acids (E) mutated to glutamine (Q)

    Depositor

    Michael Downey

    Article

    Baijal et al bioRxiv 2025.02.12.637445

    Insert

    YihI with N-term aspartic acids (D) mutated to asparagine (N) and glutamic acids (E) mutated to glutamine (Q)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    N-term aspartic acids (D) mutated to asparagine (…

    Promoter

    Available Since

    May 7, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pYihI-N-term D-A/E-L

    Plasmid

    #233097

    Purpose

    Expression of GST-YihI with N-term aspartic acids (D) mutated to alanine (A) and glutamic acids (E) mutated to leucine (L)

    Depositor

    Michael Downey

    Article

    Baijal et al bioRxiv 2025.02.12.637445

    Insert

    YihI with N-term aspartic acids (D) mutated to alanine (A) and glutamic acids (E) mutated to leucine (L)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    N-term aspartic acids (D) mutated to alanine (A) …

    Promoter

    Available Since

    May 7, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. pRAR051

    Plasmid

    #185029

    Purpose

    400 nt l31 promoter region, l31 5'UTR with nt. 47-51 and 80-84 mutated, 90 nt of l31 coding region, glycine linker, sfGFP, trrnB

    Depositor

    Julius Lucks

    Article

    Rasmussen et al Nucleic Acids Res. 2022 Dec 9;50(22):127

    Insert

    L31-sfGFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Nucleotides 47-51 & 80-84 mutated rpmE 5'…

    Promoter

    Available Since

    July 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRAR052

    Plasmid

    #185030

    Purpose

    400 nt l31 promoter region, l31 5'UTR with nt. 56-61 and 68-73 mutated, 90 nt of l31 coding region, glycine linker, sfGFP, trrnB

    Depositor

    Julius Lucks

    Article

    Rasmussen et al Nucleic Acids Res. 2022 Dec 9;50(22):127

    Insert

    L31-sfGFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Nucleotides 56-61 and 68-73 mutated in rpmE 5…

    Promoter

    Available Since

    July 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pRAR104

    Plasmid

    #185034

    Purpose

    400 nt l31 promoter region, l31 5'UTR A74 and A76 deletion, 90 nt of l31 coding region, glycine linker, sfGFP, trrnB

    Depositor

    Julius Lucks

    Article

    Rasmussen et al Nucleic Acids Res. 2022 Dec 9;50(22):127

    Insert

    L31-sfGFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    A74 and A76 deletion of rpmE 5'UTR, Only fir…

    Promoter

    Available Since

    July 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pRAR105

    Plasmid

    #185035

    Purpose

    400 nt l31 promoter region, l31 5'UTR G52 and G79 deletion, 90 nt of l31 coding region, glycine linker, sfGFP, trrnB

    Depositor

    Julius Lucks

    Article

    Rasmussen et al Nucleic Acids Res. 2022 Dec 9;50(22):127

    Insert

    L31-sfGFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    G52 and G79 deletion of rpmE 5'UTR, Only fir…

    Promoter

    Available Since

    July 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pRAR107

    Plasmid

    #185037

    Purpose

    400 nt l31 promoter region, l31 5'UTR AGA74-76GAG mutation, 90 nt of l31 coding region, glycine linker, sfGFP, trrnB

    Depositor

    Julius Lucks

    Article

    Rasmussen et al Nucleic Acids Res. 2022 Dec 9;50(22):127

    Insert

    L31-sfGFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    AGA74-76GAG mutation of rpmE 5'UTR, Only fir…

    Promoter

    Available Since

    July 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge