We narrowed to 10,031 results for: pET
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Plasmid#207390PurposeExpression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cellsDepositorInsertB-evoSpCas9
UseCRISPRTags6xHisExpressionBacterialMutationM495V, Y515N, K526E, R661Q, amino acids 1005-1013…PromoterT7Available sinceSept. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET-51b(+) ChemoG 3.2
Plasmid#193803PurposeProtein production of ChemoG3.2 in bacteriaDepositorInsertChemoG 3.2
Tags10xHisExpressionBacterialMutationEGFP:A206K, T225R; HT7: L271EPromoterT7Available sinceJune 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET-11a (GB1 WT - A34F)
Plasmid#136609PurposeExpresses GB1 WT mutant (A34F)DepositorInsertGB1 WT mutant (A34F)
Tags6x-HistagExpressionBacterialMutationMutation from GB1 WT: A34FPromoterT7 promoterAvailable sinceOct. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET-DEUT RUVBL1 Delete
Plasmid#159144PurposeExpression and protien purification of mutated RUVBL1DepositorInsertRUVBL1 (RUVBL1 Human)
ExpressionBacterialMutationamino acid 1-463, Glu134-Glu237 mutated to GPPGAvailable sinceSept. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET-TALE-mCherry-15b
Plasmid#138287PurposeExpress tTALE-mCherryDepositorInsert7-mer tTALE-mCherry
UseTALENTags6x His-tagExpressionBacterialPromoterT7 promoterAvailable sinceMarch 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET-FLAG-eSpCas9-plus
Plasmid#126774PurposeExpression of increased fidelity eSpCas9-plus in bacterial cells. Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with close to the same fidelity as eSpCas9.DepositorInserteSpCas9-plus
UseCRISPRTags3xFLAG, 6xHis, MBP, and NLSExpressionBacterialMutationK848A, R1060A; amino acids 1005-1013 replaced wit…PromoterT7Available sinceMarch 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET-Himar-dCas9-mammalian
Plasmid#138414PurposeProtein purification of Himar-dCas9 with 3x-FLAG, SV40 NLS, and 6x-His tagDepositorInsertHimar-dCas9
Tags3xFLAG, 6xHis, and SV40 NLSExpressionBacterialPromoterT7Available sinceMarch 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET-28a(+)-MVK mazei
Plasmid#108947Purposeheterologous expression of MVK of M. mazei in E. coliDepositorInsertMevalonate kinase (MVK)
TagsHis6-TagExpressionBacterialAvailable sinceSept. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET-DEST42-ARL6-V5-His6
Plasmid#67422PurposeExpression of hArl6 with C-term V5 and His6 tag in E. coliDepositorAvailable sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET-Galectin-8 linker deletion
Plasmid#68806PurposeExpress Galectin-8 linker deletion protein in Bacterial cellsDepositorInsertGalectin-8 (LGALS8 Human)
TagsN-terminal 6-histidine-tagExpressionBacterialMutationNo mutationPromoterT7Available sinceSept. 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET-22HT-MSI1(7-103)
Plasmid#60357PurposeExpress the RRM1 of mouse MSI1 in bacterial cells for protein purificationDepositorInsertmouse MSI1 (7-103) (Msi1 Mouse)
TagsHis and TEV protease siteExpressionBacterialMutationcontains amino acids 7-103 of Msi1PromoterT7Available sinceNov. 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
HOXC13(human) GSTfusion-pET
Plasmid#8680DepositorPublicationInsertHOXC13 (HOXC13 Human)
TagsGSTExpressionBacterialMutationmakes protein by Coomassie blue stainingAvailable sinceMay 30, 2006AvailabilityAcademic Institutions and Nonprofits only -
HOXA9 (murine) no-tags pET
Plasmid#8493DepositorAvailable sinceMay 30, 2006AvailabilityAcademic Institutions and Nonprofits only -
pET-Duet1-His-TEV-mCherry-p62
Plasmid#199780PurposeUsed for the expression of mCherry labelled p62 (SMC Internal No. 391).DepositorAvailable sinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMS1088
Plasmid#80159PurposepET-Duet for bacterial expression of Cys-NuG2(4x)-Ald and co-expression of Mycobacterium tuberculosis Formylglycine Generating EnzymeDepositorInsertHisSUMO-Cys-4xNuG2-Ald
TagsHisSUMOPromoterT7Available sinceAug. 24, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pET MBP mCerulean LIC cloning vector (MBP-mCerulean)
Plasmid#29751DepositorTypeEmpty backboneTags6xHis, AviTag (biotin), MBP, TEV cleavage site, a…ExpressionBacterialAvailable sinceAug. 17, 2011AvailabilityAcademic Institutions and Nonprofits only -
pETM33_Nsp16
Plasmid#156482PurposeBacterial expression of Sars-CoV2 Nsp16 protein with His-tag and GST-tagDepositorInsertNsp16 (ORF1ab Severe acute respiratory syndrome coronavirus 2, Synthetic)
TagsHis-GSTExpressionBacterialMutationGene insert is codon optimized for Ecoli.PromoterT7/LacOAvailable sinceAug. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET32bplus_trxAChEwt
Plasmid#83916PurposeEncodes an E. coli codon optimized version of wild type human acetylcholinesteraseDepositorInsertShort form of hAChE, codon optimised for E.coli expression (ACHE Human, Synthetic)
TagsHis, S-tag, and TRXExpressionBacterialMutationcodon optimised for E.coli expressionPromoterT7 promoterAvailable sinceMarch 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET22b_EcSSB_1
Plasmid#78203PurposeExpression of single-cysteine variant of single-stranded DNA-binding protein protein, EcSSB, as scaffold for ssDNA biosensorDepositorInsertssb (G26C)
ExpressionBacterialMutationG26C (please see depositor comment below)PromoterT7Available sinceJuly 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET His6 TEV cloning vector with BioBrick polycistronic restriction sites (9B)
Plasmid#48284DepositorTypeEmpty backboneTagsHis6ExpressionBacterialAvailable sinceSept. 24, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by