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Plasmid#176252PurposepNOC episomal plasmid harboring the humanized fnCas12a gene sequence tagged with Nlux and CRISPR array with spacers 1 and 2 targeting the Nitrate reductase gene of N. oceanica IMET1 separated by fullDepositorInserthumanized fnCas12a
UseCRISPR, Luciferase, and Synthetic Biology ; Expre…TagsluciferaseExpressionMutationPromoterAvailable sinceNov. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNOC_hlbCas12a-Nlux
Plasmid#176240PurposepNOC episomal plasmid harboring the humanized lbCas12a gene sequence tagged with Nlux under the control of Ribi promoterDepositorInserthumanized lbCas12a
UseCRISPR, Luciferase, and Synthetic Biology ; Expre…TagsluciferaseExpressionMutationPromoterRibosomal subunitAvailable sinceNov. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
px458_2A_GFP_sgRNA_EIF2AK2
Plasmid#106108PurposeCRISPR knockout. Expresses Cas9, EGFP, and sgRNA targeting EIF2AK2DepositorInsertgRNA targeting EIF2AK2 (EIF2AK2 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceFeb. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBZCas13b-HEPN
Plasmid#89901PurposeBacterial expression for bzCas13b and crRNA with both HEPN domains mutated. New spacers can be cloned by digesting with BsaI.DepositorInsertCas13b
UseTagsExpressionBacterialMutationR116A/H121A/R1177A/H1182APromoterLacAvailable sinceMay 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBEST-p70a-UTR3-Nterm 6xHis mRFP-T500
Plasmid#92317PurposeExpression of NTerm 6xHis tagged mRFP using the p70a promoter and UTR3DepositorInsertOptimized NTerm 6xHis tagged monomeric RFP (mRFP)
UseSynthetic Biology; Cell free protein synthesis (c…Tags6xHisExpressionBacterialMutationPromoterp70aAvailable sinceJuly 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Syn-CsChR-GFP
Plasmid#58841PurposeAAV expression of CsChR-GFP under the Syn promoterDepositorInsertCsChR-GFP
UseAAVTagsGFPExpressionMammalianMutationPromoterSynAvailable sinceAug. 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-double floxed-ClopHensorN-WPRE-HGHpA
Plasmid#193728PurposeCre-activated AAV expression of ClopHensorN for measuring intracellular chloride and pH in genetically-defined cell typesDepositorInsertClopHensorN
UseAAVTagsStrep-II (N terminal on insert)ExpressionMammalianMutationPromoterEF1aAvailable sinceMarch 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
px458_2A_GFP_sgRNA_CELF2
Plasmid#106102PurposeCRISPR knockout. Expresses Cas9, EGFP, and sgRNA targeting CELF2DepositorInsertgRNA targeting CELF2 (CELF2 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceFeb. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
px458_2A_GFP_sgRNA_CELF1
Plasmid#106101PurposeCRISPR knockout. Expresses Cas9, EGFP, and sgRNA targeting CELF1DepositorInsertgRNA targeting CELF1 (CELF1 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceFeb. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBEST-p70a-UTR1-Nterm 6xHis mRFP-T500
Plasmid#92316PurposeExpression of NTerm 6xHis tagged mRFP using the p70a promoter and UTR1DepositorInsertOptimized NTerm 6xHis tagged monomeric RFP (mRFP)
UseSynthetic Biology; Cell free protein synthesis (c…Tags6xHisExpressionBacterialMutationPromoterp70aAvailable sinceAug. 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmTral-ADF_E
Plasmid#146206PurposeInsect Expression of DmTral-ADFDepositorInsertDmTral-ADF (tral Fly)
UseTagsExpressionInsectMutationthree silent mutations A198C, A387G, A1437G and 4…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmTral-FAF_E
Plasmid#146207PurposeInsect Expression of DmTral-FAFDepositorInsertDmTral-FAF (tral Fly)
UseTagsExpressionInsectMutationthree silent mutations A198C, A387G, A1437G and 4…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmTral-FDA_E
Plasmid#146208PurposeInsect Expression of DmTral-FDADepositorInsertDmTral-FDA (tral Fly)
UseTagsExpressionInsectMutationthree silent mutations A198C, A387G, A1437G and 4…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmTral-S13AI15AD30KT35A_E
Plasmid#146217PurposeInsect Expression of DmTral-S13AI15AD30KT35ADepositorInsertDmTral-S13AI15AD30KT35A (tral Fly)
UseTagsExpressionInsectMutationtwo silent mutations A198C, A387G and a mutation …PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmTral-S13AI15AR21A_E
Plasmid#146218PurposeInsect Expression of DmTral-S13AI15AR21ADepositorInsertDmTral-S13AI15AR21A (tral Fly)
UseTagsExpressionInsectMutationtwo silent mutations A198C, A387G and a mutation …PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmTral-R21A_E
Plasmid#146220PurposeInsect Expression of DmTral-R21ADepositorInsertDmTral-R21A (tral Fly)
UseTagsExpressionInsectMutationtwo silent mutations A198C, A387G and a mutation …PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmTral-LSm-V5His6_B
Plasmid#145996PurposeInsect Expression of DmTral-LSmDepositorInsertDmTral-LSm (tral Fly)
UseTagsExpressionInsectMutationone silent mutation A198C compared to the sequenc…PromoterAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmTral-E23K_B
Plasmid#145982PurposeInsect Expression of DmTral-E23KDepositorInsertDmTral-E23K (tral Fly)
UseTagsExpressionInsectMutationtwo silent mutations A198C, A387G and a mutation …PromoterAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmTral-LSm-E23K-V5His6_B
Plasmid#145983PurposeInsect Expression of DmTral-LSm-E23KDepositorInsertDmTral-LSm-E23K (tral Fly)
UseTagsExpressionInsectMutationone silent mutation A198C compared to the sequenc…PromoterAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmTral-LSm-R21E-V5His6_B
Plasmid#145984PurposeInsect Expression of DmTral-LSm-R21EDepositorInsertDmTral-LSm-R21E (tral Fly)
UseTagsExpressionInsectMutationone silent mutation A198C compared to the sequenc…PromoterAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only