We narrowed to 11,223 results for: NSI;

1. pCDNA3.1(+)-NEMOm

   Plasmid

   #189930

   Purpose

   Mammalian expression of calcium sensor with ultra-high dynamics and sensitivity

   Depositor

   Yubin Zhou

   Article

   Li et al Nat Methods. 2023 Jun;20(6):918-924. doi

   Insert

   NEMOs
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCDNA3.1(+)-NEMOs

   Plasmid

   #189931

   Purpose

   Mammalian expression of calcium sensor with ultra-high dynamics and sensitivity

   Depositor

   Yubin Zhou

   Article

   Li et al Nat Methods. 2023 Jun;20(6):918-924. doi

   Insert

   NEMOs
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCDNA3.1(+)-NEMOc

   Plasmid

   #189933

   Purpose

   Mammalian expression of calcium sensor with ultra-high dynamics and sensitivity

   Depositor

   Yubin Zhou

   Article

   Li et al Nat Methods. 2023 Jun;20(6):918-924. doi

   Insert

   NEMOc
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pIRE4-Azi

   Plasmid

   #105829

   Purpose

   Plasmid encoding for the translational pair that enables efficent incoporation of p-Azido-phenylalanine in response to the amber codon into proteins in mammmalian cells

   Depositor

   Irene Coin

   Article

   Seidel et al Elife. 2017 Aug 3;6. doi: 10.7554/eLife.

   Inserts

   Humanized EAziRS
   4 copies of the U6-BstYam tRNA cassette
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   (Y37L, D182S, F183M, L186A, D265R)TyrRS

   Promoter

   PGK and U6

   Available since

   March 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pXJ-His-VTCN1

   Plasmid

   #232664

   Purpose

   transient expresses VTCN1 in mammalian cells

   Depositor

   Chen-Yang Shen

   Article

   Chou et al Cell Rep. 2024 Jan 23;43(1):113641. doi:

   Insert

   VTCN1 (VTCN1 Human)
   

   Use

   Tags

   His and His (retained after signal peptide cleava…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCDNA3.1(+)-NEMOb

   Plasmid

   #189934

   Purpose

   Mammalian expression of calcium sensor with ultra-high dynamics and sensitivity

   Depositor

   Yubin Zhou

   Article

   Li et al Nat Methods. 2023 Jun;20(6):918-924. doi

   Insert

   NEMOb
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pBR_attB(bxb)_lox

   Plasmid

   #183762

   Purpose

   Bxb1-specific donor plasmid for the cloning of payloads <20 kb

   Depositor

   Richard Davis

   Article

   Blanch-Asensio et al Cell Rep Methods. 2022 Sep 22;2(10):1003

   Type

   Empty backbone

   Use

   Cre/Lox and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. Mito-TurboID

   Plasmid

   #200960

   Purpose

   Transiently expresses mitochondria localized TurboID with V5 tags

   Depositor

   Keriann Backus

   Article

   Yan et al Cell Chem Biol. 2023 Jul 20;30(7):811-82

   Insert

   mito-TurboID
   

   Use

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA3.0 FLAG TRPV4

   Plasmid

   #45751

   Depositor

   Robert Lefkowitz

   Article

   Shukla et al J Biol Chem. 2010 Sep 24;285(39):30115-2

   Insert

   TRPV4 (Trpv4 Rat)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 21, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Golgi-TurboID

    Plasmid

    #200959

    Purpose

    Transiently expresses golgi localized TurboID with HA tags

    Depositor

    Keriann Backus

    Article

    Yan et al Cell Chem Biol. 2023 Jul 20;30(7):811-82

    Insert

    Golgi-TurboID
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pYDS2.0_AT118-H

    Plasmid

    #222846

    Purpose

    Yeast-display plasmid encoding Nb. AT118-H, a humanized nanobody antagonist of the human Angiotensin II type I receptor (AT1R)

    Depositor

    Andrew Kruse

    Article

    Skiba et al Nat Chem Biol. 2024 May 14. doi: 10.1038

    Insert

    AT118-H
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Sept. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. p15_attB(bxb)_lox

    Plasmid

    #183764

    Purpose

    Bxb1-specific donor plasmid for the cloning of payloads (~10 - 50 kb)

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al Cell Rep Methods. 2022 Sep 22;2(10):1003

    Type

    Empty backbone

    Use

    Cre/Lox and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pEF-XMAS-1xStop

    Plasmid

    #164411

    Purpose

    Transient Reporter for Editing Enrichment (TREE) Plasmid. GFP turns on with adenosine base editing (ABE). 1 stop codon.

    Depositor

    Xiao Wang

    Article

    Brookhouser et al BMC Biol. 2020 Dec 14;18(1):193. doi: 10

    Insert

    mCherry
    

    Use

    CRISPR and Synthetic Biology

    Tags

    T2A-GFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1-alpha

    Available since

    Aug. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. Bxb1-GT_CAG_3xTurboGFP-NLS

    Plasmid

    #229777

    Purpose

    Bxb1-GT donor plasmid for integrating a transcription unit for constitutive expression of nuclear-localised TurboGFP (3 copies)

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

    Insert

    3x TurboGFP
    

    Use

    Synthetic Biology

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    March 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. Yellow Cameleon-Nano15/pBig

    Plasmid

    #51962

    Purpose

    Expression of Yellow Cameleon-Nano15, an ultrasensitive Ca2+ indicator with Kd = 15 nM, in Dictyostelium

    Depositor

    Takeharu Nagai

    Article

    Horikawa et al Nat Methods. 2010 Sep;7(9):729-32. doi:

    Insert

    Yellow Cameleon-Nano15
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCW57-MCS1-P2A-MCS2-mDux

    Plasmid

    #138320

    Purpose

    Dox-inducible codon-optimized mouse Dux

    Depositor

    Yi Zhang

    Article

    Fu et al Nat Cell Biol. 2019 Jul;21(7):835-844. d

    Insert

    Dux (Dux Mouse)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    TRE

    Available since

    Feb. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. mbYFPQS

    Plasmid

    #80742

    Purpose

    membrane-targeted biosensor for monitoring changes in intracellular chloride

    Depositor

    Susan Ingram, Susan Amara

    Article

    Watts et al PLoS One. 2012;7(4):e35373. doi: 10.1371

    Insert

    YFPQS
    

    Use

    Tags

    palmitoylation signal sequence of neuromodulin

    Expression

    Mammalian

    Mutation

    H148Q (CAC to CAG) and V163S (GTG to AGC).

    Promoter

    CMV

    Available since

    Aug. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

18. pUC57-HBB-nLuc

    Plasmid

    #175432

    Purpose

    Used to generate IVT RNA for translation extracts

    Depositor

    Britt Glaunsinger

    Article

    Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

    Insert

    HBB-nLuc
    

    Use

    Luciferase

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Bxb1-GA_CAG-TurboGFP

    Plasmid

    #229781

    Purpose

    Bxb1-GA donor plasmid for integrating a transcription unit for constitutive expression of nuclear-localised TurboGFP

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

    Insert

    TurboGFP
    

    Use

    Synthetic Biology

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    March 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pRetroX-Tight-MCS_PGK-GpNLuc

    Plasmid

    #70185

    Purpose

    Constiutive mammalian expression of the bioluminescent GpNLuc LumiFluor reporter for single-cell analysis and sensitive optical imaging of living subjects..

    Depositor

    Antonio Amelio

    Article

    Schaub et al Cancer Res. 2015 Sep 30. pii: canres.353

    Type

    Empty backbone

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available since

    Dec. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge