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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 1,283 results for: abo.1

Showing: 1241 - 1260 of 1283 results

  1. pRVL-6

    Plasmid
    #104584
    Purpose
    Contains human IgG1 CH1/mutated CH2/CH3 domains, for cloning of antibody VH domains using SapI restriction enzyme. Abolished Fc-mediated immune effector functions
    Depositor
    Daniel Christ
    Article
    Vazquez-Lombardi et al Methods Mol Biol. 2018;1827:313-334. doi
    Insert
    Human IgG1 CH1/CH2/CH3 (mutated, no effector functions) (IGHG1 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    L234A/L235E/G237A and K322A/P331S
    Promoter
    CMV
    Available since
    Jan. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    104584-plasmid-map-sequence-id-198608

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  2. pJL NarL YdfI Flag

    Plasmid
    #225650
    Purpose
    Expresses NarL with a mutated DNA binding domain (YdfI). For use with NarX chimeras and reporter plasmids from this study.
    Depositor
    Neha Kamat
    Article
    Peruzzi et al Proc Natl Acad Sci U S A. 2023 May 9;120
    Insert
    NarL-YdfI (narL Synthetic)
    Use
    Cell free expression
    Tags
    FLAG
    Expression
    Bacterial
    Mutation
    NarL with native DNA-binding domain replaced with…
    Promoter
    T7
    Available since
    Dec. 2, 2024
    Availability
    Academic Institutions and Nonprofits only
    225650-plasmid-map-sequence-id-454890

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  4. pUWR-CadTS-C

    Plasmid
    #58326
    Purpose
    Destination vector for inserting ubi-CadTS control to Drosophila melanogaster genome
    Depositor
    Denise Montell
    Article
    Cai et al Cell. 2014 May 22;157(5):1146-59. doi: 1
    Insert
    E-cadherin tension sensor control
    Use
    Tags
    Expression
    Insect
    Mutation
    Promoter
    poly-ubiquitin
    Available since
    Aug. 5, 2014
    Availability
    Academic Institutions and Nonprofits only

  5. pBbE1k-3

    Plasmid
    #71722
    Purpose
    tyrosine hydroxylase from mouse, dihydropterine reductase from human, pterin-4a-carbinolamine dehydratase from human
    Depositor
    Taek Soon Lee
    Article
    Satoh et al Metab Eng. 2012 Nov;14(6):603-10. doi: 1
    Inserts
    dihydropterine reductase (QDPR Human)
    pterin-4a-carbinolamine dehydratase (PCBD1 Human)
    Use
    Tags
    Expression
    Mutation
    Promoter
    pTrc
    Available since
    April 13, 2016
    Availability
    Academic Institutions and Nonprofits only
    71722-plasmid-map-sequence-id-129984

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  6. pUC-hU6-crBACH1-array_EF1a-BFP

    Plasmid
    #224787
    Purpose
    BACH1 targeting crRNA array for RfxCas13d expressed from multiple hU6 promoter and reporter BFP protein expressed from EF1a promoter
    Depositor
    Gabor Balazsi
    Article
    Wan et al ACS Synth Biol. 2024 Oct 18;13(10):3212-
    Insert
    crBACH1-1, crBACH1-2, crBACH1-3,
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    hU6 and hU6-2xTetO
    Available since
    Oct. 31, 2024
    Availability
    Academic Institutions and Nonprofits only
    224787-plasmid-map-sequence-id-450827

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  7. PB-TO-hNIL

    Plasmid
    #172113
    Purpose
    Piggybac Tet-ON plasmid for differentiating hiPSCs into lower motor neurons via NGN2, ISL1, and LHX3 expression
    Depositor
    iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward
    Article
    Pantazis et al Cell Stem Cell. 2022 Dec 1;29(12):1685-1
    Inserts
    hNGN2 (NEUROG2 Human)
    hISL1 (ISL1 Human)
    hLHX3 (LHX3 Human)
    puroR-T2A-mycNLS-mTagBFP2
    Use
    Tags
    T2A-mycNLS-mTagBFP2
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a and TRE3G
    Available since
    April 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    172113-plasmid-map-sequence-id-359519

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  8. J-HARS-4

    Plasmid
    #166529
    Purpose
    scFv of a human scaffold targeting Histidyl-tRNA synthetase. Antigen coverage aa 1-509 of 509, full-length
    Depositor
    Susanne Gräslund
    Article
    Preger et al J Biol Chem. 2020 Oct 9;295(41):13981-13
    Insert
    single chain-Fv, scFv
    Use
    Recombinant antibody expression
    Tags
    3xFLAG, His6 and OmpA leader sequence
    Expression
    Bacterial
    Mutation
    Promoter
    LacZ
    Available since
    April 28, 2021
    Availability
    Academic Institutions and Nonprofits only
    166529-plasmid-map-sequence-id-331465

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  9. L-SARS-7

    Plasmid
    #166527
    Purpose
    scFv of a human scaffold targeting Seryl-tRNA synthetase. Antigen coverage aa 1-514 of 514, full-length
    Depositor
    Susanne Gräslund
    Article
    Preger et al J Biol Chem. 2020 Oct 9;295(41):13981-13
    Insert
    single chain-Fv, scFv
    Use
    Recombinant antibody expression
    Tags
    3xFLAG, His6 and OmpA leader sequence
    Expression
    Bacterial
    Mutation
    Promoter
    LacZ
    Available since
    April 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    166527-plasmid-map-sequence-id-329199

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  10. L-KARS-5

    Plasmid
    #166530
    Purpose
    scFv of a human scaffold targeting Lysyl-tRNA synthetase. Antigen coverage aa 1-597 of 597, full-length
    Depositor
    Susanne Gräslund
    Article
    Preger et al J Biol Chem. 2020 Oct 9;295(41):13981-13
    Insert
    single chain-Fv, scFv
    Use
    Recombinant antibody expression
    Tags
    3xFLAG, His6 and OmpA leader sequence
    Expression
    Bacterial
    Mutation
    Promoter
    LacZ
    Available since
    April 20, 2021
    Availability
    Academic Institutions and Nonprofits only
    166530-plasmid-map-sequence-id-329204

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  11. O-MARS-11

    Plasmid
    #166541
    Purpose
    scFv of a human scaffold targeting Methionyl-tRNA synthetase. Antigen coverage aa 1-225 of 900
    Depositor
    Susanne Gräslund
    Article
    Preger et al J Biol Chem. 2020 Oct 9;295(41):13981-13
    Insert
    single chain-Fv, scFv
    Use
    Recombinant antibody expression
    Tags
    3xFLAG, His6 and OmpA leader sequence
    Expression
    Bacterial
    Mutation
    Promoter
    LacZ
    Available since
    April 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    166541-plasmid-map-sequence-id-329234

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  12. L-NARS-2

    Plasmid
    #166532
    Purpose
    scFv of a human scaffold targeting Asparaginyl-tRNA synthetase. Antigen coverage aa 1-458 of 458, full-length
    Depositor
    Susanne Gräslund
    Article
    Preger et al J Biol Chem. 2020 Oct 9;295(41):13981-13
    Insert
    single chain-Fv, scFv
    Use
    Recombinant antibody expression
    Tags
    3xFLAG, His6 and OmpA leader sequence
    Expression
    Bacterial
    Mutation
    Promoter
    LacZ
    Available since
    April 5, 2021
    Availability
    Academic Institutions and Nonprofits only
    166532-plasmid-map-sequence-id-329490

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  13. O-AARS-51

    Plasmid
    #166539
    Purpose
    scFv of a human scaffold targeting Alanyl-tRNA synthetase. Antigen coverage aa 1-455 of 968
    Depositor
    Susanne Gräslund
    Article
    Preger et al J Biol Chem. 2020 Oct 9;295(41):13981-13
    Insert
    single chain-Fv, scFv
    Use
    Recombinant antibody expression
    Tags
    3xFLAG, His6 and OmpA leader sequence
    Expression
    Bacterial
    Mutation
    Promoter
    LacZ
    Available since
    April 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    166539-plasmid-map-sequence-id-329232

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  14. L-WARS-26

    Plasmid
    #166528
    Purpose
    scFv of a human scaffold targeting Tryptophanyl-tRNA synthetase. Antigen coverage aa 1-1471 of 471, full-length
    Depositor
    Susanne Gräslund
    Article
    Preger et al J Biol Chem. 2020 Oct 9;295(41):13981-13
    Insert
    single chain-Fv, scFv
    Use
    Recombinant antibody expression
    Tags
    3xFLAG, His6 and OmpA leader sequence
    Expression
    Bacterial
    Mutation
    Promoter
    LacZ
    Available since
    March 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    166528-plasmid-map-sequence-id-328276

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  15. O-AARS-32

    Plasmid
    #166540
    Purpose
    scFv of a human scaffold targeting Alanyl-tRNA synthetase. Antigen coverage aa 1-968 of 968, full-length
    Depositor
    Susanne Gräslund
    Article
    Preger et al J Biol Chem. 2020 Oct 9;295(41):13981-13
    Insert
    single chain-Fv, scFv
    Use
    Recombinant antibody expression
    Tags
    3xFLAG, His6 and OmpA leader sequence
    Expression
    Bacterial
    Mutation
    Promoter
    LacZ
    Available since
    April 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    166540-plasmid-map-sequence-id-329233

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  18. GRM1-DuET

    Plasmid
    #213300
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    GRM1 (GRM1 Human)
    Use
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, T7
    Available since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213300-plasmid-map-sequence-id-430906

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  19. pAAV2-CAG-3xNLS-AausFP1

    Plasmid
    #191096
    Purpose
    To express a bright green FP to label eucaryotic cell nuclei. To be used in tissue-clearing methods and other fluorescent microscopy methods
    Depositor
    Pantelis Tsoulfas, Murray Blackmore
    Article
    Wang et al Elife. 2022 Jul 15;11. pii: 76254. doi:
    Insert
    3xNLS-AausFP1
    Use
    AAV and Synthetic Biology
    Tags
    Three repeats of the nuclear localizzation seque…
    Expression
    Mammalian
    Mutation
    Optimized to Human codon usage
    Promoter
    CMV enhancer and CAG
    Available since
    Dec. 16, 2022
    Availability
    Academic Institutions and Nonprofits only
    191096-plasmid-map-sequence-id-380123

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Showing: 1241 - 1260 of 1283 results