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pcDNA3.1-flag-UTR

Plasmid

#183234

Purpose

Mammalian expression empty backbone

Depositor

Jorge Ferrer

Article

Beucher et al Nat Cell Biol. 2022 Oct 6. pii: 10.1038/

Type

Empty backbone

Tags

FLAG tag

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pAB035a

Plasmid

#79190

Purpose

Directed Evolution of Protein-Protein Interactions

Depositor

David Liu

Article

Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

Insert

Plac62 gIII, luxAB; Ptet rpoZ-SH2ABL1

Expression

Bacterial

Promoter

Plac62; Ptet

Available Since

Dec. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pCS2-zfKitlgb

Plasmid

#168200

Purpose

Express zebrafish Kitlgb, mRNA synthesis

Depositor

Petr Bartunek

Article

Oltova et al Blood Adv. 2020 Dec 8;4(23):5915-5924. d

Insert

Kitlgb (kitlgb Zebrafish)

Use

Vertebrate expression (zebrafish, chicken, xenopu…

Expression

Mammalian

Mutation

I85V, V222I, and D233N- please see depositor comm…

Available Since

Nov. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pAB031a

Plasmid

#79221

Purpose

Directed Evolution of Protein-Protein Interactions

Depositor

David Liu

Article

Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

Insert

PlacZ HA4-Zif268; PlacIq lacI

Expression

Bacterial

Promoter

PlacZ; PlacIq

Available Since

March 30, 2017

Availability

Academic Institutions and Nonprofits only

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pAB029f

Plasmid

#79220

Purpose

Directed Evolution of Protein-Protein Interactions

Depositor

David Liu

Article

Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

Insert

PlacZ lambda cI-SH2ABL1; PlacIq lacI

Expression

Bacterial

Promoter

PlacZ; PlacIq

Available Since

June 25, 2021

Availability

Academic Institutions and Nonprofits only

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Mad WT

Plasmid

#22996

Depositor

Edward De Robertis

Article

Eivers et al PLoS One. 2009 . 4(8):e6543.

Insert

Mad (Mad Fly)

Tags

FLAG

Expression

Mammalian

Available Since

June 7, 2010

Availability

Academic Institutions and Nonprofits only
pAB043c

Plasmid

#79223

Purpose

Directed Evolution of Protein-Protein Interactions

Depositor

David Liu

Article

Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

Insert

PlacZ lambda cI-p38-alpha; PlacIq lacI

Expression

Bacterial

Promoter

PlacZ; PlacIq

Available Since

Oct. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pETM6-Pc4CL-PhCHS-CmCHI

Plasmid

#73397

Purpose

Expresses three enzymes to convert phenylpropanoic acids to flavanones in vivo

Depositor

Mattheos Koffas

Article

Jones et al Metab Eng. 2016 Feb 6;35:55-63. doi: 10.

Inserts

Pc4CL
PhCHS
CmCHI

Expression

Bacterial

Promoter

T7

Available Since

March 1, 2016

Availability

Academic Institutions and Nonprofits only

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pAB042b

Plasmid

#79193

Purpose

Directed Evolution of Protein-Protein Interactions

Depositor

David Liu

Article

Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

Insert

Plac62 gIII, luxAB; Ptet rpoZ-MBPoff7

Expression

Bacterial

Promoter

Plac62; Ptet

Available Since

June 28, 2018

Availability

Academic Institutions and Nonprofits only

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pAB042d

Plasmid

#79194

Purpose

Directed Evolution of Protein-Protein Interactions

Depositor

David Liu

Article

Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

Insert

Plac62 gIII, luxAB; Ptet rpoZ-p38_2_3

Expression

Bacterial

Promoter

Plac62; Ptet

Available Since

March 12, 2018

Availability

Academic Institutions and Nonprofits only

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pFL-TK-AID3'UTR-MUT

Plasmid

#19987

Depositor

Nina Papavasiliou

Article

Teng et al Immunity. 2008 May . 28(5):621-9.

Insert

AID 3' UTR (Aicda Mouse)

Expression

Mammalian

Mutation

Mutation

Available Since

Jan. 9, 2009

Availability

Academic Institutions and Nonprofits only

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Mad GSK3

Plasmid

#22995

Depositor

Edward De Robertis

Article

Eivers et al PLoS One. 2009 . 4(8):e6543.

Insert

Mad (Mad Fly)

Tags

FLAG

Expression

Mammalian

Mutation

S208A, S204A

Available Since

June 7, 2010

Availability

Academic Institutions and Nonprofits only
Mad MM

Plasmid

#22997

Depositor

Edward De Robertis

Article

Eivers et al PLoS One. 2009 . 4(8):e6543.

Insert

Mad (Mad Fly)

Tags

FLAG

Expression

Mammalian

Mutation

S212A

Available Since

June 7, 2010

Availability

Academic Institutions and Nonprofits only
pAB043a

Plasmid

#79222

Purpose

Directed Evolution of Protein-Protein Interactions

Depositor

David Liu

Article

Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

Insert

PlacZ lambda cI-MBP; PlacIq lacI

Expression

Bacterial

Promoter

PlacZ; PlacIq

Available Since

April 15, 2017

Availability

Academic Institutions and Nonprofits only

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pAB082f

Plasmid

#79238

Purpose

Directed Evolution of Protein-Protein Interactions

Depositor

David Liu

Article

Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

Insert

Ppro1 rpoZ-Cry1Ac-d23A

Expression

Bacterial

Promoter

Ppro1

Available Since

Nov. 8, 2016

Availability

Academic Institutions and Nonprofits only

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pDEST-GBKT7

Plasmid

#248655

Purpose

Empty vector for generating yeast 2-hybrid GAL4 DNA binding domain fusion constructs by Gateway cloning. It may also be used as an empty vector experimental control.

Depositor

Sarah Assmann, John Doonan

Article

Rossignol et al J Cell Sci. 2007 Oct 15;120(Pt 20):3678-

Type

Empty backbone

Use

Yeast 2-hybrid

Tags

GAL4 binding domain, Myc

Expression

Yeast

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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pDEST-GADT7

Plasmid

#248657

Purpose

Empty vector for generating yeast 2-hybrid GAL4 activation domain fusion constructs by Gateway cloning. It may also be used as an empty vector experimental control.

Depositor

Sarah Assmann, John Doonan

Article

Rossignol et al J Cell Sci. 2007 Oct 15;120(Pt 20):3678-

Type

Empty backbone

Use

Yeast 2-hybrid

Tags

GAL4 activation domain, HA

Expression

Yeast

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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L1p6_barley_sgRNA_scaffold

Plasmid

#231033

Purpose

Cloning vector to clone sgRNA in scaffold under TaU6 promoter for Cas9 mediated CRISPR for goldengate level1 position 6

Depositor

Wilma van Esse

Article

Winkelmolen et al Plant Physiol. 2025 Aug 4;198(4):kiaf309

Insert

Scaffold for Cas9 mediated CRISPR

Use

CRISPR

Expression

Bacterial

Promoter

TaU6

Available Since

March 7, 2025

Availability

Academic Institutions and Nonprofits only

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L1p5_barley_sgRNA_scaffold

Plasmid

#231032

Purpose

Cloning vector to clone sgRNA in scaffold under TaU6 promoter for Cas9 mediated CRISPR for goldengate level1 position 5

Depositor

Wilma van Esse

Article

Winkelmolen et al Plant Physiol. 2025 Aug 4;198(4):kiaf309

Insert

Scaffold for Cas9 mediated CRISPR

Use

CRISPR

Expression

Bacterial

Promoter

TaU6

Available Since

March 7, 2025

Availability

Academic Institutions and Nonprofits only

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L1p4_barley_sgRNA_scaffold

Plasmid

#231031

Purpose

Cloning vector to clone sgRNA in scaffold under TaU6 promoter for Cas9 mediated CRISPR for goldengate level1 position 4

Depositor

Wilma van Esse

Article

Winkelmolen et al Plant Physiol. 2025 Aug 4;198(4):kiaf309

Insert

Scaffold for Cas9 mediated CRISPR

Use

CRISPR

Expression

Bacterial

Promoter

TaU6

Available Since

March 7, 2025

Availability

Academic Institutions and Nonprofits only

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