... Schaepe, J., Du, P. P., Lotfy, P., Bassik, M. C., Bintu, L., Bhatt, A. S., & Hsu, P. D. (2022). Systematic... 29556040 Chen, P. J., Hussmann, J. A., Yan, J., Knipping, F., Ravisankar, P., Chen, P., Chen, C., Nelson...target DNA PCR P olymerase C hain R eaction; Used to amplify a specific sequence of DNA pegRNA P rime E dit...Kleinstiver, B. P., Prew, M. S., Tsai, S. Q., Topkar, V. V., Nguyen, N. T., Zheng, Z., Gonzales, A. P. W., Li,...Manchado, E., Concepcion, C. P., Bonetti, C., Vidigal, J. A., Han, Y., Ogrodowski, P., Crippa, A., Rekhtman,...Haft, D. H., Horvath, P., Moineau, S., Mojica, F. J. M., Terns, R. M., Terns, M. P., White, M. F., Yakunin.... V., Randolph, P. B., Davis, J. R., Sousa, A. A., Koblan, L. W., Levy, J. M., Chen, P. J., Wilson, C....

...Expression Codon PI 31186 pEVOL-pAzF p-azidohenylalanine RS M. jannaschii p-azido-l-phenylalanine Bacterial...Peter Schultz 31190 pEVOL-pBpF p-benzoylphenylalanine RS M. jannaschii p-benzoyl-l-phenylalanine Bacterial..._AfTyrRS(p-I-Phe)_AlvtRNA-ΔNPyl(8)(CGA)_AftRNA-Tyr(A01)(CUA) 1R26PylRS and AfTyrRS CbzK and p-I-Phe Bacterial...pKPY514 phenylalanyl-tRNA synthetase subunit E. coli p-azido-L-phenylalanine (Azf) C. elegans ATG David Tirrell... phenylalanyl-tRNA synthetase (CePheRS) C.elegans p-azido-L-phenylalanine (Azf) C. elegans ATG David Tirrell...Huiwang Ai 73544 pEvol-pAcFRS.2.t1 pAcFRS.2.t1 E. coli p-acetyl-l-phenylalanine (pAcF) Bacterial TAG Farren...Isaacs 73545 pEvol-pAcFRS.1.t1 pAcFRS.1.t1 E. coli p-acetyl-l-phenylalanine (pAcF) Bacterial TAG Farren...

...samples: $$P={(H + L)\over A}*100$$ Where: P = Purity, % H = Area of HC peak L ...

... C, Deveau H, Richards M, Boyaval P, Moineau S, Romero DA, Horvath P. 2007. CRISPR provides acquired resistance... M, Loehr J, Jacquet K, Huard CC, Roques C, Herst P, Côté J, Doyon Y. 2015. A Scalable Genome-Editing-...4. PMID: 27096365 Ma H, Naseri A, Reyes-Gutierrez P, Wolfe SA, Zhang S, Pederson T. 2015. Multicolor CRISPR...Barrangou R, Brouns SJ, Charpentier E, Haft DH, Horvath P, Moineau S, Mojica FJ, Terns RM, Terns MP, White MF...Rev Microbiol . 13(11):722-36. PMID: 26411297 Mali P, Yang L, Esvelt KM, Aach J, Guell M, DiCarlo JE, Norville...Abudayyeh OO, Slaymaker IM, Makarova KS, Essletzbichler P, Volz SE, Joung J, van der Oost J, Regev A, Koonin...PMID: 26422227 Zetsche B, Heidenreich M, Mohanraju P, Fedorova I, Kneppers J, DeGennaro EM, Winblad N, ...

...PubMed (Link opens in a new window) Tovote P, Esposito MS, Botta P, Chaudun F, Fadok JP, Markovic M, Wolff... Deisseroth K, Zhao F, Luo MH, Gong L, He M, Zhou P, Paninski L, Li B. 2017. The central amygdala controls...Venkataraju K, Straub C, Wang W, Robertson K, Osten P, Sabatini BL. 2019. Distinct Cortical-Thalamic-Striatal...

...Chen, P., Casanova, J. E., Cherfils, J., Dacks, J. B., Lambright, D. G., Lee, F. S., Randazzo, P. A., ...

...Methods Mol Biol, 2011. 767: p. 411-8. PubMed PMID: 21822892. 33. Shi, Y., P. Kirwan, and F.J. Livesey, ...reprogramming and pluripotency. Nature, 2006. 441(7097): p. 1061-7. PubMed PMID: 16810240. 2. Takahashi, K., ...fibroblasts by defined factors. Cell, 2007. 131(5): p. 861-72. PubMed PMID: 18035408. 3. Takahashi, K. and... cultures by defined factors. Cell, 2006. 126(4): p. 663-76. PubMed PMID: 16904174. 4. Yu, J., et al.,...from human somatic cells. Science, 2007. 318(5858): p. 1917-20. PubMed PMID: 18029452. 5. Stadtfeld, M.,...without viral integration. Science, 2008. 322(5903): p. 945-9. PubMed PMID: 18818365. PubMed Central PMCID...vectors. Proc Natl Acad Sci U S A, 2011. 108(34): p. 14234-9. PubMed PMID: 21821793. PubMed Central PMCID...

...Obstet Gynaecol, 2004. 18(6): p. 827-42. PubMed PMID: 15582541. 5. Seale, P., A. Asakura, and M.A. Rudnicki...Medicine. Geburtshilfe Frauenheilkd, 2015. 75(12): p. 1239-1242. PubMed PMID: 26726264. PubMed Central ...pluripotency and nuclear reprogramming. Cell, 2008. 132(4): p. 567-82. PubMed PMID: 18295576. PubMed Central PMCID...reprogramming. Adv Biochem Eng Biotechnol, 2009. 114: p. 185-99. PubMed PMID: 19343304. PubMed Central PMCID...potential of muscle stem cells. Dev Cell, 2001. 1(3): p. 333-42. PubMed PMID: 11702945. 6. Cowan, C.A., et...from human blastocysts. N Engl J Med, 2004. 350(13): p. 1353-6. PubMed PMID: 14999088. 7. Turetsky, T., et...preimplantation genetic diagnosis. Hum Reprod, 2008. 23(1): p. 46-53. PubMed PMID: 17989069. 8. Takahashi, K. and...

...019-1711-4 Chen, P. J., Hussmann, J. A., Yan, J., Knipping, F., Ravisankar, P., Chen, P.-F., Chen, C., ....2021.09.018 Nelson, J. W., Randolph, P. B., Shen, S. P., Everette, K. A., Chen, P. J., Anzalone, A. V., An, M..... V., Randolph, P. B., Davis, J. R., Sousa, A. A., Koblan, L. W., Levy, J. M., Chen, P. J., Wilson, C....j.cell.2014.02.001 Yan, J., Oyler-Castrillo, P., Ravisankar, P., Ward, C. C., Levesque, S., Jing, Y., Simpson...s41587-021-01039-7 Nishimasu, H., Ran, F. A., Hsu, P. D., Konermann, S., Shehata, S. I., Dohmae, N., Ishitani... Emily P. Bentley...

...Cells by Centrifugation. Stem Cells Int, 2016. 2016: p. 1329459. PubMed PMID: 27579041. PubMed Central PMCID...gene expression signatures. Cell Res, 2011. 21(3): p. 518-29. PubMed PMID: 21243013. PubMed Central PMCID...gene therapy for SCID-X1. Science, 2003. 302(5644): p. 415-9. PubMed PMID: 14564000. 5. Seki, T., S. Yuasa...T cells and Sendai virus. Nat Protoc, 2012. 7(4): p. 718-28. PubMed PMID: 22422317. 6. Diecke, S., et ...pluripotent stem cells. Korean J Intern Med, 2014. 29(5): p. 547-57. PubMed PMID: 25228828. 7. Okita, K., et al... peripheral blood cells. Stem Cells, 2013. 31(3): p. 458-66. PubMed PMID: 23193063. 8. Ehrhardt, A., et...vectors for gene therapy. Curr Gene Ther, 2008. 8(3): p. 147-61. PubMed PMID: 18537590. 9. Mack, A.A., et ...

... S L L T C G D V E E N P G P P2A: (GSG) A T N F S L L K Q A G D V E E N P G P E2A: (GSG) Q C T N Y...L K L A G D V E S N P G P F2A: (GSG) V K Q T L N F D L L K L A G D V E S N P G P * (GSG) residues...

...019-1711-4 Chen, P. J., Hussmann, J. A., Yan, J., Knipping, F., Ravisankar, P., Chen, P.-F., Chen, C., ...00868-w Nelson, J. W., Randolph, P. B., Shen, S. P., Everette, K. A., Chen, P. J., Anzalone, A. V., An, M..... V., Randolph, P. B., Davis, J. R., Sousa, A. A., Koblan, L. W., Levy, J. M., Chen, P. J., Wilson, C....s41551-024-01227-1 Yan, J., Oyler-Castrillo, P., Ravisankar, P., Ward, C. C., Levesque, S., Jing, Y., Simpson...Jennifer Tsang in October 2019 and updated by Emily P. Bentley in December 2024. References and Resources...Neugebauer, M. E., An, M., Davis, J. R., Randolph, P. B., McElroy, A., Gao, X. D., Raguram, A., Richter..., L., Vakulskas, C. A., Walker, J. A., Kadina, A. P., Zepeda, A. E., Holden, K., Ma, H., Xie, J., Gao,...

...strain expressing two reporters and dCas9 under a P-tet promoter integrated in the chromosome at phage...

...References and Resources References Gelsinger, D. R., Vo, P. L. H., Klompe, S. E., Ronda, C., Wang, H. H., & Sternberg...doi.org/10.1038/s41596-023-00927-3 Klompe, S. E., Vo, P. L. H., Halpin-Healy, T. S., & Sternberg, S. H. (2019...https://doi.org/10.1073/pnas.1709035114 Witte, I. P., Lampe, G. D., Eitzinger, S., Miller, S. M., Berríos...King, R. T., Stringham, O. G., Gelsinger, D. R., Vo, P. L. H., Chen, A. T., Tolar, J., Osborn, M. J., Sternberg... Emily P. Bentley...

...window) References Botman D, de Groot DH, Schmidt P, Goedhart J, Teusink B. In vivo characterisation of...

...( 68307 ) at the central position. Introducing Hi-P for screening phosphorylation-dependent protein-protein...phosphorylation-dependent interactions. Barber KW, Muir P, Szeligowski RV, Rogulina S, Gerstein M, Sampson JR...

..., E., Serack, F., Harvey, J. W., Brauer, P. P., Pirtle, C. P., Coffey, A., Howard, M., Kamath, N., … &... evolved optimizations for prime editing By Emily P. Bentley The David Liu Lab has made two recent deposits...prime editing installs two sequences containing attB/P recombinase landing sites on opposite DNA strands.... 5’ flaps yields an edited sequence with the attB/P landing site. A recombinase integrates a sequence ..., Banskota, S., Davis, J. R., Newby, G. A., Chen, P. Z., Palczewski, K., & Liu, D. R. (2024). Engineered...   CHARM: A compact epigenetic silencer By Emily P. Bentley The Weissman and Gilbert Labs have previously...

...Haft, D. H., Horvath, P., Moineau, S., Mojica, F. J. M., Terns, R. M., Terns, M. P., White, M. F., Yakunin...Horvath, P., Moineau, S., Mojica, F. J. M., Scott, D., Shah, S. A., Siksnys, V., Terns, M. P., Venclovas...Abudayyeh, O. O., Gootenberg, J. S., Essletzbichler, P., Han, S., Joung, J., Belanto, J. J., Verdine, V.,..., Cox, D., Lin, S., Barretto, R., Habib, N., Hsu, P. D., Wu, X., Jiang, W., Marraffini, L. A., & Zhang..., Chen, J. S., Paez-Espino, D., Ma, E., Witte, I. P., Cofsky, J. C., Kyrpides, N. C., Banfield, J. F.,...Makarova, K. S., Acuña, L. G., Wolf, Y. I., Covarrubias, P. C., Shmakov, S. A., Silva, C., Tolstoy, I., Johnson...–2012. https://doi.org/10.1093/nar/gkz1197 Samai, P., Pyenson, N., Jiang, W., Goldberg, G. W., Hatoum-...

... Cameron, P., Coons, M. M., Klompe, S. E., Lied, A. M., Smith, S. C., Vidal, B., Donohoue, P. D., Rotstein...Haft, D. H., Horvath, P., Moineau, S., Mojica, F. J. M., Terns, R. M., Terns, M. P., White, M. F., Yakunin...10.1016/j.ggedit.2022.100013 O’Brien, R. E., Bravo, J. P., Ramos, D., Hibshman, G. N., Wright, J. T., & Taylor....024 Morisaka, H., Yoshimi, K., Okuzaki, Y., Gee, P., Kunihiro, Y., Sonpho, E., Xu, H., Sasakawa, N., ...

... of CcdB.   Figure 1: Image from Bernard, P., et al. showing the pKIL 18/19 vectors and demonstrating...guest blog entry of your own! References 1. Bernard, P. “Positive Selection of Recombinant DNA by CcdB.” ...Mar;15(6):1031-7. PubMed PMID: 7623659. 3. Bernard, P., et al. “Positive-selection vectors using the F plasmid...11;148(1):71-4. PubMed PMID: 7926841. 4. Bernard, P., et al. “The F plasmid CcdB protein induces efficient...;234(3):534-41. PubMed PMID: 8254658. 5. Bernard, P. and Couturier, M. “Cell killing by the F plasmid ...