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...019-1711-4 Chen, P. J., Hussmann, J. A., Yan, J., Knipping, F., Ravisankar, P., Chen, P.-F., Chen, C., ....2021.09.018 Nelson, J. W., Randolph, P. B., Shen, S. P., Everette, K. A., Chen, P. J., Anzalone, A. V., An, M..... V., Randolph, P. B., Davis, J. R., Sousa, A. A., Koblan, L. W., Levy, J. M., Chen, P. J., Wilson, C....j.cell.2014.02.001 Yan, J., Oyler-Castrillo, P., Ravisankar, P., Ward, C. C., Levesque, S., Jing, Y., Simpson...s41587-021-01039-7 Nishimasu, H., Ran, F. A., Hsu, P. D., Konermann, S., Shehata, S. I., Dohmae, N., Ishitani... Emily P. Bentley...

...Cells by Centrifugation. Stem Cells Int, 2016. 2016: p. 1329459. PubMed PMID: 27579041. PubMed Central PMCID...gene expression signatures. Cell Res, 2011. 21(3): p. 518-29. PubMed PMID: 21243013. PubMed Central PMCID...gene therapy for SCID-X1. Science, 2003. 302(5644): p. 415-9. PubMed PMID: 14564000. 5. Seki, T., S. Yuasa...T cells and Sendai virus. Nat Protoc, 2012. 7(4): p. 718-28. PubMed PMID: 22422317. 6. Diecke, S., et ...pluripotent stem cells. Korean J Intern Med, 2014. 29(5): p. 547-57. PubMed PMID: 25228828. 7. Okita, K., et al... peripheral blood cells. Stem Cells, 2013. 31(3): p. 458-66. PubMed PMID: 23193063. 8. Ehrhardt, A., et...vectors for gene therapy. Curr Gene Ther, 2008. 8(3): p. 147-61. PubMed PMID: 18537590. 9. Mack, A.A., et ...

... S L L T C G D V E E N P G P P2A: (GSG) A T N F S L L K Q A G D V E E N P G P E2A: (GSG) Q C T N Y...L K L A G D V E S N P G P F2A: (GSG) V K Q T L N F D L L K L A G D V E S N P G P * (GSG) residues...

...019-1711-4 Chen, P. J., Hussmann, J. A., Yan, J., Knipping, F., Ravisankar, P., Chen, P.-F., Chen, C., ...00868-w Nelson, J. W., Randolph, P. B., Shen, S. P., Everette, K. A., Chen, P. J., Anzalone, A. V., An, M..... V., Randolph, P. B., Davis, J. R., Sousa, A. A., Koblan, L. W., Levy, J. M., Chen, P. J., Wilson, C....s41551-024-01227-1 Yan, J., Oyler-Castrillo, P., Ravisankar, P., Ward, C. C., Levesque, S., Jing, Y., Simpson...Jennifer Tsang in October 2019 and updated by Emily P. Bentley in December 2024. References and Resources...Neugebauer, M. E., An, M., Davis, J. R., Randolph, P. B., McElroy, A., Gao, X. D., Raguram, A., Richter..., L., Vakulskas, C. A., Walker, J. A., Kadina, A. P., Zepeda, A. E., Holden, K., Ma, H., Xie, J., Gao,...

...References and Resources References Gelsinger, D. R., Vo, P. L. H., Klompe, S. E., Ronda, C., Wang, H. H., & Sternberg...doi.org/10.1038/s41596-023-00927-3 Klompe, S. E., Vo, P. L. H., Halpin-Healy, T. S., & Sternberg, S. H. (2019...https://doi.org/10.1073/pnas.1709035114 Witte, I. P., Lampe, G. D., Eitzinger, S., Miller, S. M., Berríos...King, R. T., Stringham, O. G., Gelsinger, D. R., Vo, P. L. H., Chen, A. T., Tolar, J., Osborn, M. J., Sternberg... Emily P. Bentley...

..., E., Serack, F., Harvey, J. W., Brauer, P. P., Pirtle, C. P., Coffey, A., Howard, M., Kamath, N., … &... evolved optimizations for prime editing By Emily P. Bentley The David Liu Lab has made two recent deposits...prime editing installs two sequences containing attB/P recombinase landing sites on opposite DNA strands.... 5’ flaps yields an edited sequence with the attB/P landing site. A recombinase integrates a sequence ..., Banskota, S., Davis, J. R., Newby, G. A., Chen, P. Z., Palczewski, K., & Liu, D. R. (2024). Engineered...   CHARM: A compact epigenetic silencer By Emily P. Bentley The Weissman and Gilbert Labs have previously...

...Haft, D. H., Horvath, P., Moineau, S., Mojica, F. J. M., Terns, R. M., Terns, M. P., White, M. F., Yakunin...Horvath, P., Moineau, S., Mojica, F. J. M., Scott, D., Shah, S. A., Siksnys, V., Terns, M. P., Venclovas...Abudayyeh, O. O., Gootenberg, J. S., Essletzbichler, P., Han, S., Joung, J., Belanto, J. J., Verdine, V.,..., Cox, D., Lin, S., Barretto, R., Habib, N., Hsu, P. D., Wu, X., Jiang, W., Marraffini, L. A., & Zhang..., Chen, J. S., Paez-Espino, D., Ma, E., Witte, I. P., Cofsky, J. C., Kyrpides, N. C., Banfield, J. F.,...Makarova, K. S., Acuña, L. G., Wolf, Y. I., Covarrubias, P. C., Shmakov, S. A., Silva, C., Tolstoy, I., Johnson...–2012. https://doi.org/10.1093/nar/gkz1197 Samai, P., Pyenson, N., Jiang, W., Goldberg, G. W., Hatoum-...

... Cameron, P., Coons, M. M., Klompe, S. E., Lied, A. M., Smith, S. C., Vidal, B., Donohoue, P. D., Rotstein...Haft, D. H., Horvath, P., Moineau, S., Mojica, F. J. M., Terns, R. M., Terns, M. P., White, M. F., Yakunin...10.1016/j.ggedit.2022.100013 O’Brien, R. E., Bravo, J. P., Ramos, D., Hibshman, G. N., Wright, J. T., & Taylor....024 Morisaka, H., Yoshimi, K., Okuzaki, Y., Gee, P., Kunihiro, Y., Sonpho, E., Xu, H., Sasakawa, N., ...

... of CcdB.   Figure 1: Image from Bernard, P., et al. showing the pKIL 18/19 vectors and demonstrating...guest blog entry of your own! References 1. Bernard, P. “Positive Selection of Recombinant DNA by CcdB.” ...Mar;15(6):1031-7. PubMed PMID: 7623659. 3. Bernard, P., et al. “Positive-selection vectors using the F plasmid...11;148(1):71-4. PubMed PMID: 7926841. 4. Bernard, P., et al. “The F plasmid CcdB protein induces efficient...;234(3):534-41. PubMed PMID: 8254658. 5. Bernard, P. and Couturier, M. “Cell killing by the F plasmid ...

...there is often discussion of “big P” and “little p” policy.  “Big P” policy is what you might typically...scientific areas. Whether you’re interested in “big P” or “little P” policy, the possibilities are endless leading... government itself. You can work to influence Big P policy from a post in non-governmental organizations...formal lobbying, or strategy setting.  The “little p” policy is non-governmental work happening at large...

...., Nalin, R., Sailland, A., Auriol, P., Vogel, T. M., & Simonet, P. (2001). Laboratory-scale evidence ...Jessica Welch in January 2017 and was updated by Emily P. Bentley in October 2024. References and Resources...Veseli, I., Moore, R. M., Kiefl, E., Moody, T., Rice, P. A., Yu, M. K., Mimee, M., Chang, E. B., Ruscheweyh...doi.org/10.1016/j.cell.2024.01.039 Garcillán-Barcia, M. P., Redondo-Salvo, S., & de la Cruz, F. (2023). Plasmid...

...Sands, J., Sahasrabudhe, A., Anikeeva, P., & Chandrakasan, A. P. (2020). Modular Optoelectronic System...EMBC44109.2020.9175600 Park, S., Loke, G., Fink, Y., & Anikeeva, P. (2019). Flexible fiber-based optoelectronics for ...Niroui, F., Fink, Y., Chandrakasan, A., … Anikeeva, P. (2023). Multifunctional microelectronic fibers enable...

...j.cell.2020.04.031 Zimmermann I, Egloff P, Hutter CAJ, Kuhn BT, Bräuer P, Newstead S, Dawson RJP, Geertsma ...s41596-020-0304-x Zimmermann I, Egloff P, Hutter CA, Arnold FM, Stohler P, Bocquet N, Hug MN, Huber S, Siegrist...Siegrist M, Hetemann L, Gera J, Gmür S, Spies P, Gygax D, Geertsma ER, Dawson RJ, Seeger MA (2018) Synthetic...

... protein interactions. J Cell Biol, 2003. 163(2): p. 257-69. PubMed PMID: 14581454. PubMed Central PMCID...microdomains of live cells. Science, 2002. 296(5569): p. 913-6. PubMed PMID: 11988576. 3. Matz, M.V., et al...nonbioluminescent Anthozoa species. Nat Biotechnol, 1999. 17(10): p. 969-73. PubMed PMID: 10504696. 4. Ohashi, T., et ... unstructured proteins. Protein Sci, 2007. 16(7): p. 1429-38. PubMed PMID: 17586775. PubMed Central PMCID...under physiologic conditions. Traffic, 2012. 13(5): p. 643-9. PubMed PMID: 22289035. PubMed Central PMCID...

...s41565-023-01419-x Deverman, B. E., Pravdo, P. L., Simpson, B. P., Kumar, S. R., Chan, K. Y., Banerjee, A...Hordeaux, J., Yuan, Y., Clark, P. M., Wang, Q., Martino, R. A., Sims, J. J., Bell, P., Raymond, A., Stanford,... A. I., Dvoryanchikov, G., Makhoul, V., Tsoulfas, P., & Chaudhari, N. (2022). Selectively Imaging Cranial...Griffiths, J. A., Ichiki, T., Mazmanian, S. K., Osborne, P. B., Keast, J. R., Miller, C. T., Fox, A. S., Chiu... N. M., Sun, J., Chen, X., Lefevre, A., Singh, V. P., Arokiaraj, C. M., Shay, T. F., Vendemiatti, J., ... A., Wu, W.-L., Yang, B., Huber, N., Pasca, S. P., & Gradinaru, V. (2016). Cre-dependent selection yields...Gore, B. B., Szelenyi, E. R., Trader, C., Balaram, P., … Tasic, B. (2021). Enhancer viruses for combinatorial...

....   References Bennardo N, Gunn A, Cheng A, Hasty P, Stark JM (2009) Limiting the Persistence of a Chromosome...10.1371/journal.pgen.1000683 Bétermier M, Bertrand P, Lopez BS (2014) Is Non-Homologous End-Joining Really...Waters CA, Strande NT, Pryor JM, Strom CN, Mieczkowski P, Burkhalter MD, Oh S, Qaqish BF, Moore DT, Hendrickson....1038/ncomms5286 Yan M-Y, Li S-S, Ding X-Y, Guo X-P, Jin Q, Sun Y-C (2020) A CRISPR-Assisted Nonhomologous...

...plasmids and plasmids in incompatibility groups N, P, Q, and W are known to have replicons with broad host...Myxococcus virescens, M. xanthus, Proteus rettgeri, P. mirabilis, Providencia stuartii, Pseudomonas spp....Paracoccous denitrificans, Pseudomonas fluorescens, P. putida, Rhizobium meliloti, R. leguminosarum by. .../10.1074/jbc.273.14.8447  Jain, A., & Srivastava, P. (2013). Broad host range plasmids. FEMS Microbiology...2.50021-0  Toh, W. K., Teo, Y. L., Tor, X. Y., Loh, P. C., & Wong, H. L. (2023). Development of constitutive...

...Kateriya, S., Adeishvili, N., Berthold, P., Ollig, D., Hegemann, P., & Bamberg, E. (2003). Channelrhodopsin...Papagiakoumou, E., Gottschalk, A., Soba, P., Emiliani, V., Engel, A. K., Hegemann, P., & Wiegert, J. S. (2021). BiPOLES...Berndt, A., Sohal, V. S., Deisseroth, K., & Hegemann, P. (2010). Ultrafast optogenetic control. Nature Neuroscience....1936192100 Tichy, A.-M., Gerrard, E. J., Sexton, P. M., & Janovjak, H. (2019). Light-activated chimeric...