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Showing: 1 - 20 of 23744 results

  1. pCMV-MbPylRS(DiZPK)

    Plasmid
    #91706
    Purpose
    express Methanosarcina barkeri pyrrolysyl-tRNA synthetase/tRNA pair in mammalian cells to incorporate the photocrosslinkers DiZPK, DiZSeK, or DiZHSeC into amber codon site on a protein of interest
    Depositor
    P. Chen
    Article
    Lin et al Angew Chem Int Ed Engl. 2013 Dec 23;52(5
    Inserts
    pylS
    pylT
    Use
    Tags
    Expression
    Mammalian
    Mutation
    T810G, C813T, C820G, T821C, T937A, A1046T, G1257A
    Promoter
    Available since
    July 20, 2017
    Availability
    Academic Institutions and Nonprofits only
    91706-plasmid-map-sequence-id-183279

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  2. pSupAR-MbPylRS(DiZPK)

    Plasmid
    #91705
    Purpose
    express Methanosarcina barkeri pyrrolysyl-tRNA synthetase/tRNA pair in E. coli to incorporate photocrosslinkers DiZPK, DiZSeK, or DiZHSeC into an in-frame amber codon site on a protein of interest
    Depositor
    P. Chen
    Article
    Zhang et al Nat Chem Biol. 2011 Sep 4;7(10):671-7. d
    Inserts
    pylS
    pylT
    Use
    Tags
    Expression
    Bacterial
    Mutation
    T810G, C813T, C820G, T821C, T937A, A1046T, G1257A
    Promoter
    Available since
    July 20, 2017
    Availability
    Academic Institutions and Nonprofits only
    91705-plasmid-map-sequence-id-183278

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  3. pDisplay-mgSrtA

    Plasmid
    #125792
    Purpose
    Transfected into cell of interest for membrane expression. The encoded mgSrtA could enable labeling of the interacting cells which is useful for recording cell-cell interactions.
    Depositor
    P. Chen
    Article
    Ge et al J Am Chem Soc. 2019 Feb 6;141(5):1833-18
    Insert
    mgSrtA
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 1, 2019
    Availability
    Academic Institutions and Nonprofits only
    125792-plasmid-map-sequence-id-258287

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  7. pDisplay-ZHER-mgSrtA

    Plasmid
    #125793
    Purpose
    for co-expression of ZHER (a HER2 binding affibody) and mgSrtA on cell membrane. For proof-of-concept monitoring and labeling of HER2 positive cells.
    Depositor
    P. Chen
    Article
    Ge et al J Am Chem Soc. 2019 Feb 6;141(5):1833-18
    Insert
    ZHER-mgSrtA
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 30, 2019
    Availability
    Academic Institutions and Nonprofits only
    125793-plasmid-map-sequence-id-245285

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  12. pSPIKE-P

    Plasmid
    #101172
    Purpose
    chimeric spike resembling V4 region of prokaryotic 16S rRNA to be used in metagenomics
    Depositor
    Philip Poole
    Insert
    V4 16S rRNA chimeric spike
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 2, 2017
    Availability
    Academic Institutions and Nonprofits only
    101172-plasmid-map-sequence-id-194323

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  16. p-OiRS3GG

    Plasmid
    #98589
    Purpose
    Expresses default target RNA (gI intron) and pheS cassette (in place of an antisense RNA probe, for golden gate cloning) in E. coli (kanR).
    Depositor
    Lydia Contreras
    Article
    Vazquez-Anderson et al Nucleic Acids Res. 2017 May 19;45(9):552
    Inserts
    group I intron
    PheS cassette to be selectively replaced by unique asRNA probes
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    pBAD and pLtetO
    Available since
    Sept. 27, 2017
    Availability
    Academic Institutions and Nonprofits only
    98589-plasmid-map-sequence-id-194130

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  18. P-d22bp

    Plasmid
    #235657
    Purpose
    B. thetaiotaomicron fusA2 expression reporter lacking 22bp Cur binding site
    Depositor
    Guy Townsend
    Article
    Kabonick et al bioRxiv [Preprint]. 2024 Nov 13:2024.11.
    Insert
    Bacteroides thetaiotaomicron fusA2 promoter lacking the 22bp Cur binding site
    Use
    Tags
    Expression
    Mutation
    The B. thetaiotaomicron fusA2 promoter lacking &q…
    Promoter
    Available since
    June 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    235657-plasmid-map-sequence-id-474231

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Showing: 1 - 20 of 23744 results