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pSHS212

Plasmid

#87346

Purpose

Cysteine-free (C80S/C574S) S. pyogenes Cas9 expression plasmid

Depositor

Jennifer Doudna

Article

Sternberg et al Nature. 2015 Nov 5;527(7576):110-3. doi:

Insert

Cas9

Tags

His-MBP

Expression

Bacterial

Mutation

C80S/C574S

Promoter

T7

Available Since

March 22, 2017

Availability

Academic Institutions and Nonprofits only

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P1

Plasmid

#87089

Purpose

This is the P1 part of the Bcl-2 promoter with a luciferase reporter in pGL3Basic plasmid

Depositor

Yohannes Tesfaigzi

Article

Bredow et al Gene. 2007 Dec 1;404(1-2):110-6. Epub 20

Type

Empty backbone

Expression

Mammalian

Promoter

Bcl-2 Promoter

Available Since

July 19, 2017

Availability

Academic Institutions and Nonprofits only
pHisrKr2

Plasmid

#22698

Depositor

Lynn Hudson

Article

Lovas et al Glia. 2001 Apr 15. 34(2):110-20.

Insert

Rat Kruppel-type protein 2 (Zfp111 Rat)

Tags

His

Expression

Bacterial

Available Since

Jan. 5, 2010

Availability

Academic Institutions and Nonprofits only
pET11a-SUMO2

Plasmid

#53142

Purpose

E.coli expression of untagged mature human SUMO2 (amino acids 1-92)

Depositor

Frauke Melchior

Article

Flotho et al Methods Mol Biol. 2012;832:93-110. doi:

Insert

SUMO2 (SUMO3 Human)

Expression

Bacterial

Mutation

mature form (amino acids 1-92)

Promoter

T7

Available Since

June 6, 2014

Availability

Academic Institutions and Nonprofits only

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pDisplay-mSA-EGFP-TM

Plasmid

#39863

Depositor

Sheldon Park

Article

Lim et al Biotechnol Bioeng. 2013 Jan;110(1):57-67

Insert

monomeric streptavidin, EGFP, TM helix

Tags

EGFP, FLAG, and HA

Expression

Mammalian

Promoter

CMV

Available Since

March 15, 2013

Availability

Academic Institutions and Nonprofits only

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330-Plakoglobin-Myc

Plasmid

#32228

Depositor

Kathleen Green

Article

Palka et al J Cell Sci. 1997 Oct;110 ( Pt 19):2359-7

Insert

Plakoglobin (JUP Human)

Tags

Myc

Expression

Mammalian

Promoter

human beta-actin

Available Since

Oct. 26, 2011

Availability

Academic Institutions and Nonprofits only

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pET11a-SUMO3

Plasmid

#53143

Purpose

E.coli expression of untagged mature human SUMO3 (amino acids 1-93)

Depositor

Frauke Melchior

Article

Flotho et al Methods Mol Biol. 2012;832:93-110. doi:

Insert

SUMO3 (SUMO2 Human)

Expression

Bacterial

Mutation

mature form (amino acids 1-93)

Promoter

T7

Available Since

June 6, 2014

Availability

Academic Institutions and Nonprofits only

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pET28a-HisYFP-Sp100

Plasmid

#53141

Purpose

E.coli expression of HisYFP tagged human Sp100 fragment

Depositor

Frauke Melchior

Article

Flotho et al Methods Mol Biol. 2012;832:93-110. doi:

Insert

Sp100A fragment (SP100 Human)

Tags

His-YFP

Expression

Bacterial

Mutation

fragment (amino acids 71-480); deletion of L75

Promoter

T7

Available Since

June 6, 2014

Availability

Academic Institutions and Nonprofits only

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P1-M-P2

Plasmid

#87091

Purpose

This is the P1-M-P2 portion of the Bcl-2 promoter with a luciferase reporter in pGL3Basic plasmid

Depositor

Yohannes Tesfaigzi

Article

Bredow et al Gene. 2007 Dec 1;404(1-2):110-6. Epub 20

Type

Empty backbone

Expression

Mammalian

Available Since

July 19, 2017

Availability

Academic Institutions and Nonprofits only
pCMV5 Flag-Smad3 (1-145)

Plasmid

#14965

Depositor

Joan Massague

Article

Chen et al Cell. 2002 Jul 12. 110(1):19-32.

Insert

Smad3 (SMAD3 Human)

Tags

Flag

Expression

Mammalian

Mutation

aa 1-145

Available Since

May 17, 2007

Availability

Academic Institutions and Nonprofits only
pCMV5 Flag-Smad3 (146-425)

Plasmid

#14966

Depositor

Joan Massague

Article

Chen et al Cell. 2002 Jul 12. 110(1):19-32.

Insert

Smad3 (SMAD3 Human)

Tags

Flag

Expression

Mammalian

Mutation

aa 146-425

Available Since

May 17, 2007

Availability

Academic Institutions and Nonprofits only
pSHS293

Plasmid

#87348

Purpose

D435C/E945C in cysteine-free (C80S/C574S) S. pyogenes Cas9 expression plasmid, lobe closure FRET construct

Depositor

Jennifer Doudna

Article

Sternberg et al Nature. 2015 Nov 5;527(7576):110-3. doi:

Insert

Cas9

Tags

His-MBP

Expression

Bacterial

Mutation

C80S/D435C/C574S/E945C

Promoter

T7

Available Since

March 27, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV5 Flag-Smad3 (220-425)

Plasmid

#14967

Depositor

Joan Massague

Article

Chen et al Cell. 2002 Jul 12. 110(1):19-32.

Insert

Smad3 (SMAD3 Human)

Tags

Flag

Expression

Mammalian

Mutation

aa 220-425

Available Since

May 17, 2007

Availability

Academic Institutions and Nonprofits only
pSHS248

Plasmid

#87354

Purpose

S867C/N1054C in cysteine-free (C80S/C574S) S. pyogenes Cas9 expression plasmid, HNH-2 FRET construct

Depositor

Jennifer Doudna

Article

Sternberg et al Nature. 2015 Nov 5;527(7576):110-3. doi:

Insert

Cas9

Tags

His-MBP

Expression

Bacterial

Mutation

C80S/C574S/S867C/N1054C

Promoter

T7

Available Since

April 25, 2017

Availability

Academic Institutions and Nonprofits only

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pP3-GFP

Plasmid

#53436

Purpose

Controls GFP expression from the P3 promoter cloned out of S. aureus RN4220 genome

Depositor

Cynthia Collins

Article

Marchand et al Biotechnol Bioeng. 2013 Nov;110(11):3003

Insert

P3 promoter

Use

Synthetic Biology

Tags

none

Expression

Bacterial

Mutation

none

Promoter

P3

Available Since

June 3, 2014

Availability

Academic Institutions and Nonprofits only

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pXylA-agrCA-I

Plasmid

#53437

Purpose

xylose-dependent expression of the type-I agrC and agrA genes of S. aureus

Depositor

Cynthia Collins

Article

Marchand et al Biotechnol Bioeng. 2013 Nov;110(11):3003

Insert

agrCA locus

Use

Synthetic Biology

Tags

none

Expression

Bacterial

Mutation

"MVQTS" sequence added to the N-terminu…

Promoter

PxylA

Available Since

June 3, 2014

Availability

Academic Institutions and Nonprofits only

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pGEX3x-hRanBP2-(IR1+M)

Plasmid

#53140

Purpose

E.coli expression of GST tagged human RanBP2 fragment with SUMO ligase activity

Depositor

Frauke Melchior

Article

Flotho et al Methods Mol Biol. 2012;832:93-110. doi:

Insert

RanBP2 fragment (RANBP2 Human)

Tags

GST

Expression

Bacterial

Mutation

fragment (amino acids 2631-2711)

Promoter

tac

Available Since

May 30, 2014

Availability

Academic Institutions and Nonprofits only

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pGEM4Z-mMcl-1

Plasmid

#32984

Depositor

Joseph Opferman

Article

Stewart et al Mol Cell Biol. 2010 Jun;30(12):3099-110.

Insert

Mcl-1 (Mcl1 Mouse)

Expression

Bacterial

Available Since

March 15, 2012

Availability

Academic Institutions and Nonprofits only
pET15b+PCNA1 P186C

Plasmid

#66173

Purpose

Expresses the P186C mutant of Sulfolobus solfataricus PCNA1

Depositor

Teruyuki Nagamune

Article

Hirakawa et al Biotechnol Bioeng. 2013 Jul;110(7):1858-

Insert

DNA polymerase sliding clamp 1

Tags

His6 tag

Expression

Bacterial

Mutation

P186C

Promoter

T7 promoter

Available Since

June 29, 2015

Availability

Academic Institutions and Nonprofits only

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Pdx-PH2/pGL3

Plasmid

#12163

Depositor

Domenico Accili

Article

Kitamura et al J Clin Invest. 2002 Dec . 110(12):1839-4

Insert

Pdx1 promoter PH2 domain

Use

Luciferase

Available Since

June 13, 2007

Availability

Academic Institutions and Nonprofits only