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Tol2-LyzC-GFP-pa-u6ac-Lats1-guides

Plasmid

#254766

Purpose

Lat1 gRNA for neutrophil specific KO in tol2 backbone

Depositor

Qing Deng

Article

Kim et al J Leukoc Biol. 2026 Feb 9;118(2):qiaf174

Insert

Lats1 (lats1 Zebrafish)

Use

CRISPR; Zebrafish expression

Promoter

Lyzc

Available Since

May 4, 2026

Availability

Academic Institutions and Nonprofits only

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Lentivirus ABE8e-V106W with non-targeting sgRNA

Plasmid

#247668

Purpose

Lentivirus genome encoding ABE8eV106W editor and Sp sgRNA cassette non-targeting control

Depositor

David Liu

Article

Gao et al Nat Biomed Eng. 2026 Apr 14. doi: 10.103

Insert

ABE8e-V106W and non-targeting sgRNA

Use

Lentiviral

Expression

Mammalian

Promoter

EFS

Available Since

April 14, 2026

Availability

Academic Institutions and Nonprofits only

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pSicoFlipped-CAG-dTomato-WPRE-bGHpA

Plasmid

#231019

Purpose

For strong lentivirus-mediated expression of dTomato

Depositor

Tomasz Nowakowski

Article

Shin et al Cell Stem Cell. 2024 Mar 7;31(3):421-432

Insert

dTomato

Use

Lentiviral

Expression

Mammalian

Promoter

CAG

Available Since

Dec. 16, 2025

Availability

Academic Institutions and Nonprofits only

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AAV_CMV-AcrIIA5_cpGR2(GGS5)-N77

Plasmid

#246048

Purpose

Expression of AcrIIA5-cpGR2 hybrid in mammalian cells; insertion before N77; enables chemogenetic control of SauCas9; contains ITRs for AAV packaging with Rep2

Depositor

Dominik Niopek

Article

Brenker et al Nucleic Acids Res. 2025 Jul 19;53(14):gk

Insert

AcrIIA5_cpGR2-N77

Use

AAV, CRISPR, and Synthetic Biology

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 15, 2025

Availability

Academic Institutions and Nonprofits only

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AcrVA1_cpGR2(GGS5)-S103ΔELS_(GGS)2-(GGS)2

Plasmid

#246051

Purpose

Expression of AcrVA1-cpGR2 hybrid in mammalian cells; insertion before S103 with deletion of surrounding amino acids ELS and GGS2 linkers; enables chemogenetic control of MbCas12a

Depositor

Dominik Niopek

Article

Brenker et al Nucleic Acids Res. 2025 Jul 19;53(14):gk

Insert

AcrVA1_cpGR2-S103ΔELS

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Mutation

ΔELS

Available Since

Dec. 15, 2025

Availability

Academic Institutions and Nonprofits only

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pPBneo-CIBN-lin-p53CT(98-393)-R273H-mNeonGreen-NLSx3

Plasmid

#241849

Purpose

Expresses a negative control of the localizer of Opto-p53 in mammalian cells.

Depositor

Kazuhiro Aoki

Article

Tsuruoka et al Cell Struct Funct. 2025 Jun 12. doi: 10.

Insert

p53 (TP53 Human)

Tags

CIBN and mNeonGreen

Expression

Mammalian

Mutation

p53 C-terminus (97-393 aa) containing R273H mutat…

Promoter

CAGGS promoter

Available Since

Sept. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pPBbsr2-NLSx3-miRFP703-dSal-p53NT(1-97)-L22Q/W23S/W53Q/F54S-lin-hCRY2

Plasmid

#241847

Purpose

Expresses a negative control of the actuator of Opto-p53 in mammalian cells.

Depositor

Kazuhiro Aoki

Article

Tsuruoka et al Cell Struct Funct. 2025 Jun 12. doi: 10.

Insert

p53 (TP53 Human)

Tags

CRY2 and miRFP703

Expression

Mammalian

Mutation

N-terminus (1-97 aa) containing L22Q/W23S/W53Q/F5…

Promoter

CAGGS promoter

Available Since

Sept. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1α-cOpn5-T2A-mcherry

Plasmid

#237857

Purpose

The transfer plasmid for packaging AAV expressing chicken OPN5 was constructed by inserting the OPN5 coding sequence into the multiple cloning site under the control of a EF1α promoter.

Depositor

Minmin Luo

Article

Dai et al Sci China Life Sci. 2022 Jul;65(7):1271-

Insert

Opn5 (OPN5 Chicken)

Use

AAV

Promoter

EF1α

Available Since

Aug. 25, 2025

Availability

Academic Institutions and Nonprofits only

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pET28 His-SUMO-CHMP2B

Plasmid

#232018

Purpose

Bacterial expression of His and SUMO tagged CHMP2B

Depositor

Franz-Ulrich Hartl

Article

Sitron et al Mol Cell. 2025 Sep 18;85(18):3505-3523.e

Insert

CHMP2B (CHMP2B Human)

Tags

6x His, SUMO

Expression

Bacterial

Available Since

Aug. 22, 2025

Availability

Academic Institutions and Nonprofits only

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pCS2+8-CYP2A6(C14A, C82A, C439A)-flag-tev-halo-his

Plasmid

#227164

Purpose

For T-REX experiments of CYP2A6 C14A, C82A, and C439A triple mutant. Almost no sensing ability to reactive electrophilic species (RES).

Depositor

Yimon Aye

Article

Liu et al Cell. 2024 Dec 26;187(26):7450-7469.e29.

Insert

CYP2A6 (CYP2A6 Human)

Tags

Flag, HaloTag, and His6

Expression

Mammalian

Mutation

changed cysteine 14, cysteine 82, and cysteine 43…

Promoter

CMV and SP6

Available Since

Aug. 19, 2025

Availability

Academic Institutions and Nonprofits only

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