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pFastBac-Dual_BNIP3(1-158aa)-EGFP-TEV-GST

Plasmid

#223765

Purpose

Expression of recombinant protein for purification

Depositor

Sascha Martens

Article

Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

Insert

BNIP3 soluble part (BNIP3 Human)

Tags

EGFP-TEV-GST

Expression

Insect

Available Since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

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pLIB-FLAG-GST(N)-RPB1-CTD(1586-1971)

Plasmid

#231721

Purpose

Protein expression in insect cells

Depositor

Jesper Svejstrup

Article

Lopez Martinez et al Mol Cell. 2025 May 15;85(10):1952-1967.e

Insert

POLR2A (POLR2A Human)

Tags

Flag-GST-3C

Expression

Insect

Mutation

RPB1:1586-1971

Available Since

May 2, 2025

Availability

Academic Institutions and Nonprofits only

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KL158 - pBlueScript II SK-T2A-eGFP-SV40pA Podxl donor

Plasmid

#232936

Purpose

Donor plasmid for knock-in of T2A-eGFP-SV40pA at the mouse Podxl locus. Used with PX459-sgRNA047_Podxl sgRNA.

Depositor

Danny Reinberg

Article

Lucero et al bioRxiv, March 19, 2025

Insert

T2A-eGFP-SV40pA with Podxl homology arms

Use

Mouse Targeting

Available Since

March 26, 2025

Availability

Academic Institutions and Nonprofits only

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AiP1387 - pAAV-AiE2158m-minBG-SYFP2-WPRE3-BGHpA

Plasmid

#230638

Purpose

AiE2158m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

Depositor

Allen Institute for Brain Science, Bosiljka Tasic

Article

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

SYFP2

Use

AAV

Promoter

minBG

Available Since

March 24, 2025

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Spike-deltaC18 R158G (GGC)

Plasmid

#212549

Purpose

Encodes SARS-CoV-2 spike R158G for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike R158G (S Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Mutation

R158G

Promoter

CAG

Available Since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pOTTC1584 - pscAAV CMV-IE secreted EGFP-THPKTEL WPRE

Plasmid

#188539

Purpose

An AAV packaging vector that expresses secreted C-CDNF under control of the EGFP promoter.

Depositor

Brandon Harvey

Insert

secreted EGFP

Use

AAV

Tags

CDNF(1-28) and THPKTEL WPRE

Promoter

CMV-IE

Available Since

Aug. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pZR158_Lenti-U6-gRNA-10xCS1-PP7_hPGK-PCP-P65-HSF1-Puro

Plasmid

#180273

Purpose

Lentiviral expression vector for CRISPRa-SAM sgRNA with 10x capture sequence, and PCP-P65-HSF1 cassette

Depositor

Alexander Marson

Article

Schmidt et al Science. 2022 Feb 4;375(6580):eabj4008.

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Feb. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pTKan-p35S::Csy4-pNOS::cogGFP (C132, JBEI-15898)

Plasmid

#110145

Purpose

Transformation and expression of Csy4 and cogGFP proteins in plants

Depositor

Dominique Loque

Article

Liang et al ACS Synth Biol. 2017 May 19;6(5):806-816

Insert

Csy4 (ATCS Mustard Weed)

Tags

cogGFP

Expression

Bacterial and Plant

Available Since

June 14, 2018

Availability

Academic Institutions and Nonprofits only

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pFastBac-Dual_BNIP3(1-158aa)-THROMBIN-GST (W18A/L21A; deltaLIR)

Plasmid

#223778

Purpose

Expression of recombinant protein for purification

Depositor

Sascha Martens

Article

Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

Insert

BNIP3 soluble part (BNIP3 Human)

Tags

Thrombin cleavage-GST

Expression

Insect

Mutation

W18A/L21A; deletion of LIR

Available Since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

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AiP15819 - pAAV-AiE0873m_3xC2-minBG-jGCaMP8s-WPRE3-BGHpA (Alias: CN5819)

Plasmid

#224204

Purpose

AiE0873m_3xC2 is an enhancer sequence, designed to drive AAV-mediated transgene expression in striatal cholinergic neurons

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

Insert

jGCaMP8s

Use

AAV

Available Since

Oct. 4, 2024

Availability

Academic Institutions and Nonprofits only

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AiP15827 - pAAV-AiE0780m-minCMV-SYFP2-WPRE3-BGHpA (Alias: CN5827)

Plasmid

#224206

Purpose

AiE0780m is an enhancer sequence, designed to drive AAV-mediated transgene expression in striatal D1 MSNs

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

Insert

SYFP2

Use

AAV

Available Since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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AiP15828 - pAAV-AiE0780m-HSP68-SYFP2-WPRE3-BGHpA (Alias: CN5828)

Plasmid

#224207

Purpose

AiE0780m is an enhancer sequence, designed to drive AAV-mediated transgene expression in striatal D1 MSNs

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

Insert

SYFP2

Use

AAV

Available Since

Oct. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pFastBac-Dual_BNIP3(1-158aa)-EGFP-TEV-GST (W18A/L21A; deltaLIR)

Plasmid

#223766

Purpose

Expression of recombinant protein for purification

Depositor

Sascha Martens

Article

Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

Insert

BNIP3 soluble part (BNIP3 Human)

Tags

EGFP-TEV-GST

Expression

Insect

Mutation

W18A/L21A; deletion of LIR

Available Since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Spike-deltaC18 deltaE156-F157+R158G (GGA)

Plasmid

#212421

Purpose

Encodes SARS-CoV-2 spike E156-F157deletion,R158G for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike E156-F157deletion,R158G (S Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Mutation

E156-F157deletion,R158G

Promoter

CAG

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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AiP15820 - pAAV-AiE0873m_3xC2-minBG-RiboL1-jGCaMP8s-WPRE3-BGHpA (Alias: CN5820)

Plasmid

#224205

Purpose

AiE0873m_3xC2 is an enhancer sequence, designed to drive AAV-mediated transgene expression in striatal cholinergic neurons

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

Insert

RiboL1-jGCaMP8s

Use

AAV

Available Since

Oct. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pFastBac-Dual_BNIP3(1-158aa)-THROMBIN-GST (E44A L47A D49A A50K Q51A; deltaWIPI2)

Plasmid

#223777

Purpose

Expression of recombinant protein for purification

Depositor

Sascha Martens

Article

Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

Insert

BNIP3 soluble part (BNIP3 Human)

Tags

Thrombin cleavage-GST

Expression

Insect

Mutation

E44A L47A D49A A50K Q51A; disrupts WIPI2 binding

Available Since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

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pMo130-TelR

Plasmid

#50799

Purpose

Suicide vector for generating chromosomal deletions in multidrug-resistant bacteria

Depositor

Kim Lee Chua

Article

Amin et al BMC Microbiol. 2013 Jul 13;13:158. doi:

Insert

tellurite-resistance cassette

Use

Suicide vector

Promoter

PCs12

Available Since

March 10, 2014

Availability

Academic Institutions and Nonprofits only

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pJAK1.A

Plasmid

#29460

Depositor

Zakaria Hmama

Article

Sun et al Plasmid. 2009 Nov . 62(3):158-65.

Type

Empty backbone

Use

Mycobacteria expression

Expression

Bacterial

Promoter

Hsp60

Available Since

Nov. 8, 2011

Availability

Academic Institutions and Nonprofits only

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pJAK2.D

Plasmid

#29465

Depositor

Zakaria Hmama

Article

Sun et al Plasmid. 2009 Nov . 62(3):158-65.

Type

Empty backbone

Use

Mycobacteria expression

Tags

6xHis

Expression

Bacterial

Promoter

Acetamide promoter

Available Since

March 31, 2011

Availability

Academic Institutions and Nonprofits only

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pJAK1.B

Plasmid

#29462

Depositor

Zakaria Hmama

Article

Sun et al Plasmid. 2009 Nov . 62(3):158-65.

Type

Empty backbone

Use

Mycobacteria expression

Tags

M. tuberculosis FbpB signal sequence

Expression

Bacterial

Promoter

Hsp60

Available Since

Nov. 8, 2011

Availability

Academic Institutions and Nonprofits only

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