We narrowed to 4,739 results for: Drosophila

1. Comp AlphaPS3

   Plasmid

   #14071

   Depositor

   Richard Hynes

   Article

   Stark et al Development. 1997 Nov . 124(22):4583-94.

   Insert

   Drosphila Comp Alpha PS3 Integrin (scb Fly)
   

   Expression

   Bacterial

   Available Since

   March 2, 2007

   Availability

   Academic Institutions and Nonprofits only

2. pUAST-attB-yki:V5-S111A, S168A, S250A

   Plasmid

   #85620

   Purpose

   Expresses V5 tagged triple mutant yki in Drosophila.

   Depositor

   Kenneth Irvine

   Article

   Oh et al Oncogene. 2009 Apr 30;28(17):1916-27. do

   Insert

   yki-V5-S111A,S168,S250A
   

   Tags

   V5

   Expression

   Insect

   Available Since

   May 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAct-dCBEevoCDA1

   Plasmid

   #195515

   Purpose

   Ubiquitous expression of dCBEevoCDA1 in Drosophila

   Depositor

   Michael Boutros

   Article

   Doll et al Sci Adv. 2023 Sep;9(35):eadj1568. doi: 1

   Insert

   dCBE-evoCDA1
   

   Use

   CRISPR

   Expression

   Insect

   Promoter

   act5C

   Available Since

   Sept. 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pIHEU-MCS

   Plasmid

   #58375

   Purpose

   UAS vector to express gene of interest in Drosophila

   Depositor

   Chun Han

   Article

   Sapar et al Cell Rep. 2018 Aug 28;24(9):2273-2286. d

   Type

   Empty backbone

   Expression

   Insect

   Available Since

   Jan. 23, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. GFP-Rab9

   Plasmid

   #31736

   Depositor

   Ron Vale

   Article

   Huckaba et al J Biol Chem. 2011 Mar 4;286(9):7457-67.

   Insert

   Rab9 (Rab9 Fly)
   

   Tags

   GFP

   Expression

   Insect

   Promoter

   Actin

   Available Since

   Sept. 21, 2011

   Availability

   Academic Institutions and Nonprofits only

6. K560GFP

   Plasmid

   #129761

   Purpose

   Expresses Drosophila Kinesin-1 truncated after coil 1 at 560 and tagged with GFP for single-molecule motor assays

   Depositor

   William Hancock

   Insert

   KHC (Khc Fly)
   

   Tags

   GFP and His6

   Expression

   Bacterial

   Mutation

   Truncation at 559

   Available Since

   Nov. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJET1.2-STOP-dsRed

   Plasmid

   #60944

   Purpose

   Drosophila, Splicing acceptor, STOP, dsRED, RFP, SV40 terminator, flanked attP. section marker, assemble with homology arms to make Donor plasmid for CRISPR.

   Depositor

   Frank Schnorrer

   Article

   Zhang et al G3 (Bethesda). 2014 Oct 15. pii: g3.114.

   Insert

   dsRED
   

   Use

   Unspecified

   Promoter

   3XP3

   Available Since

   Nov. 19, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEntr_PE2

   Plasmid

   #149548

   Purpose

   Gateway entry clone with PE2 enzyme (with stop codon)

   Depositor

   Norbert Perrimon

   Article

   Bosch et al Proc Natl Acad Sci U S A. 2021 Jan 5;118

   Insert

   PE2
   

   Use

   CRISPR; Gateway shuttle vector

   Available Since

   Aug. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. p3xP3-EGFP.vas-int.NLS

   Plasmid

   #60948

   Purpose

   Drosophila, p3xP3-EGFP.vas-int.NLS.attB was digested SpeI to get rid of the attB site. used for RMCE. CRISPR.

   Depositor

   Frank Schnorrer

   Article

   Zhang et al G3 (Bethesda). 2014 Oct 15. pii: g3.114.

   Insert

   vas-phiC31 integrase.NLS
   

   Use

   Rmce

   Promoter

   vasa

   Available Since

   Nov. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

10. pAct-GW-HygroR

    Plasmid

    #149610

    Purpose

    Gateway compatible expression plasmid for Drosophila and establishing stable cell lines by Hygromycin selection. Actin5c ubiquitous promoter.

    Depositor

    Norbert Perrimon

    Article

    Bosch et al Proc Natl Acad Sci U S A. 2021 Jan 5;118

    Insert

    Gateway cassette (ccdb + Chlor.R.)
    

    Expression

    Insect

    Promoter

    Act5c

    Available Since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. KRigor980

    Plasmid

    #129763

    Purpose

    Expresses full-length Drosophila Kinesin-1 with rigor mutation (R210A) for adhering microtubules to surfaces

    Depositor

    William Hancock

    Insert

    KHC (Khc Fly)
    

    Tags

    His6

    Expression

    Bacterial

    Mutation

    R210A

    Available Since

    Nov. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMT-HA-STX1

    Plasmid

    #89310

    Purpose

    Expresses HA tagged syntaxin 1 in Drosophila cells

    Depositor

    Andrew Peden

    Insert

    STX1 (Syx1A Fly)
    

    Tags

    HA tag

    Expression

    Insect

    Promoter

    P-MT (Metallothionein)

    Available Since

    Aug. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. K560_AviC_coBirA

    Plasmid

    #129765

    Purpose

    Expresses Drosophila Kinesin-1 truncated at 560 and biotinylated at tail for single-molecule assays

    Depositor

    William Hancock

    Insert

    KHC (Khc Fly)
    

    Tags

    Avi-tag GLNDIFEAQKIEWH and His6

    Expression

    Bacterial

    Mutation

    Truncation at 559

    Available Since

    Nov. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMT-TAK1-V5-His

    Plasmid

    #63753

    Purpose

    Expresses drosophila TAK1 tagged with V5 and 6xHis epitopes, under control of metallothionein promoter

    Depositor

    Cyril Gueydan

    Insert

    TGF-β activated kinase 1 (Tak1 Fly)
    

    Tags

    V5-His

    Expression

    Insect

    Promoter

    pMT

    Available Since

    April 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. act-AsCpf1

    Plasmid

    #73917

    Purpose

    expression plasmid of AsCpf1

    Depositor

    Simon Bullock

    Article

    Port et al Nat Methods. 2016 Oct;13(10):852-4. doi:

    Insert

    hAsCpf1
    

    Tags

    NLS-3xHA

    Expression

    Insect

    Promoter

    act5C

    Available Since

    March 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pACU

    Plasmid

    #58373

    Purpose

    UAS vector to express gene of interest in Drosophila

    Depositor

    Chun Han, Yuh-Nung Jan

    Article

    Han et al Proc Natl Acad Sci U S A. 2011 Jun 7;108

    Type

    Empty backbone

    Expression

    Insect

    Available Since

    June 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pTSTEP

    Plasmid

    #72334

    Purpose

    Tissue-specifically swappable (C-terminal TagRFPT-to-EGP) genome tagging plasmid with Golic+ selection cassette for fully transgenic gene targeting (Drosophila melanogaster)

    Depositor

    Avital Rodal

    Article

    Koles et al Biol Open. 2015 Dec 23. pii: bio.016089.

    Insert

    RRS-TagRFPT-p10-[Golic+] RRS-EGFP
    

    Use

    CRISPR

    Expression

    Insect

    Available Since

    Jan. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. Hy_pMT EGFP SV40 pA

    Plasmid

    #69911

    Purpose

    Expresses EGFP in Drosophila

    Depositor

    Jeremy Wilusz

    Article

    Kramer et al Genes Dev. 2015 Oct 8.

    Insert

    EGFP
    

    Expression

    Insect

    Promoter

    Metallothionein Promoter (pMT)

    Available Since

    Oct. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDsRed-TSTEP_v2

    Plasmid

    #72336

    Purpose

    Tissue-specifically swappable (C-terminal TagRFPT-to-EGFP) genome tagging plasmid with dsRed selection marker for injection mediated gene targeting (Drosophila melanogaster)

    Depositor

    Avital Rodal

    Article

    Koles et al Biol Open. 2015 Dec 23. pii: bio.016089.

    Insert

    RRS-TagRFPT-p10-[3xP3dsRed]-RRS-EGFP
    

    Available Since

    Jan. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. K560_AviN_coBirA

    Plasmid

    #129766

    Purpose

    Expresses Drosophila Kinesin-1 truncated at 560 and biotinylated on the head for single-molecule assays

    Depositor

    William Hancock

    Insert

    KHC (Khc Fly)
    

    Tags

    Avi-tag GLNDIFEAQKIEWH and His6

    Expression

    Bacterial

    Mutation

    Truncation at 559

    Available Since

    Nov. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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