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Plasmid#231196PurposeCleaveIL-10DepositorInsertmono-hIL10 (R5A11M)-rens (IL10 Human)
UseTagsExpressionMammalianMutationN36I, N110I, K117N, F129LPromoterAvailable sinceJan. 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
CA253 CMVTO-rens-3GS-IL10Ra-c-myc
Plasmid#231213PurposeCleaved IL-10Ra-mycDepositorInsertrens-3GS-IL10Ra-c-myc (IL10 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
CA-0202 CMVTO-hIL6-rens.1_Del
Plasmid#231225PurposeCleaveIL-6_DelDepositorInserthIL6-rens.1_Del (IL6 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
CA-0045 CMVTO-mono-hIL10 (R5A11M)
Plasmid#231190PurposeMutant human IL-10 monomerDepositorInsertmono-hIL10 (R5A11M) (IL10 Human)
UseTagsExpressionMammalianMutationN36I, N110I, K117N, F129LPromoterAvailable sinceJan. 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
CA-0058 CMVTO-SumIL2-rens-3GS-IL2Rb
Plasmid#231223PurposeCageIL-2 (C-term 15 AA)DepositorInsertSumIL2-rens-3GS-IL2Rb (IL2 Human)
UseTagsExpressionMammalianMutationL80F, R81D, L85V, I86V, I92FPromoterAvailable sinceJan. 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
CA-0063 CMVTO-SumIL2-5GS-rens-IL2Rb
Plasmid#231221PurposeCageIL-2 (N-term 25 AA)DepositorInsertSumIL2-5GS-rens-IL2Rb (IL2 Human)
UseTagsExpressionMammalianMutationL80F, R81D, L85V, I86V, I92FPromoterAvailable sinceJan. 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
CA-0061 CMVTO-SumIL2-2GS-rens-IL2Rb
Plasmid#231219PurposeCageIL-2 (N-term 10 AA)DepositorInsertSumIL2-2GS-rens-IL2Rb (IL2 Human)
UseTagsExpressionMammalianMutationL80F, R81D, L85V, I86V, I92FPromoterAvailable sinceJan. 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-syn-F14F15S-jGCaMP7s
Plasmid#178514PurposeAAV vector for Flpo recombinase dependent jGCaMP7sDepositorInsertjGCaMP7s
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceDec. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO/MACROD2
Plasmid#172579PurposeFor generation of FlpIn lines with untagged MACROD2DepositorInsertMACROD2 (MACROD2 Human)
UseTagsnoneExpressionMammalianMutationPromoterCMVAvailable sinceApril 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
CMVTO-RBDK65E-R89L-nTEVp
Plasmid#179670PurposeMutant Ras Binding Domain split-nTEVPDepositorInsertCRaf RBD protein (RAF1 Human)
UseTagsnTEVPExpressionMammalianMutationK65E, R89LPromoterAvailable sinceMarch 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
CMVTO-RBDK65E-R89L-cTEVp
Plasmid#179671PurposeMutant Ras Binding Domain split-cTEVPDepositorInsertCRaf RBD protein (RAF1 Human)
UseTagscTEVPExpressionMammalianMutationK65E, R89LPromoterAvailable sinceMarch 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-LAP-BUB1
Plasmid#115903PurposeUsed for expression of siRNA-resistant BUB1-WT from the FRT site of FlpIn cells upon addition of doxycyclin.DepositorInsertBUB1 (BUB1 Human)
UseTagsEYFPExpressionMammalianMutationPromoterAvailable sinceOct. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-hygro-eGFP-STOP-HEC1
Plasmid#114026PurposeInducible expression of eGFP-tagged HEC1 in FLPin Trex cell lines. Due to insertion of a STOP-codon after the eGFP-tag, this plasmid only expressed eGFP.DepositorInserteGFP-STOP-HEC1 (NDC80 Human)
UseTagsExpressionMammalianMutationStart codon of Hec1 is mutated to stop codonPromoterAvailable sinceSept. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1α-FRT-FLEX-GtACR2-EYFP-Kv4.2LL
Plasmid#114373Purposeexpresses Flpo recombinase-dependent GtACR2-Kv4.2LL, which targets GtACR2 to the somatodendritic compartmentDepositorInsertGtACR2-EYFP-Kv4.2LL (NEWENTRY )
UseAAVTagsEYFP and Kv4.2LLExpressionMammalianMutationPromoterEf1aAvailable sinceAug. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1α-FRT-FLEX-GtACR2-EYFP-Kv2.1C
Plasmid#114375Purposeexpresses Flpo recombinase-dependent GtACR2-Kv2.1C, which targets GtACR2 to the somatodendritic compartmentDepositorInsertGtACR2-EYFP-Kv2.1C (NEWENTRY )
UseAAVTagsEYFP and Kv2.1CExpressionMammalianMutationPromoterEf1aAvailable sinceAug. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1α-FRT-FLEX-GtACR2-EYFP-Nlgn1C
Plasmid#114372Purposeexpresses Flpo recombinase-dependent GtACR2-Nlgn1C, which targets GtACR2 to the somatodendritic compartmentDepositorInsertGtACR2-EYFP-Nlgn1C (NEWENTRY )
UseAAVTagsEYFP and Nlgn1CExpressionMammalianMutationPromoterEf1aAvailable sinceAug. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDEST14-ARL5A
Plasmid#67378PurposeExpression of native hArl5 in E. coliDepositorInsertARL5A (ARL5A Human)
UseTagsExpressionBacterialMutationbp 70 C to T; silent mutationPromoterT7Available sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-ArgiNLS-mGreenLantern
Plasmid#220596PurposeConstitutive expression of a single-cell discriminating version of mGreenLantern fluorescent protein.DepositorInsertmGreenLantern
UseAAVTagsArgiNLSExpressionMammalianMutationPromoterEF1aAvailable sinceJuly 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pK037.CAG-FRT-stop-FRT-RFP-ires-tTA-WPRE (Supernova)
Plasmid#85038PurposeFor Flpe/FRT-based Supernova-RFP, pK037 should be used with pK036.DepositorInsertFRT-stop-FRT
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEF5/FRT/DEST-3xHA/mGli2
Plasmid#51250PurposeEncodes N-3xHA-TEV-mouseGli2-CDepositorInsertGli2 (Gli2 Mouse)
UseFlpin systemTags3xHA-TEVExpressionMammalianMutationPromoterEF1aAvailable sinceFeb. 10, 2014AvailabilityAcademic Institutions and Nonprofits only