Addgene

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ZJOM108

Plasmid

#133665

Purpose

Integration of mTagBFP2-Tlg1 as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast late golgi/early endosome.

Depositor

Zhiping Xie

Article

Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

Insert

p1k-mTagBFP2-TLG1 (TLG1 Budding Yeast)

Use

Tags

mTagBFP2

Expression

Yeast

Mutation

Promoter

Available since

April 22, 2020

Availability

Academic Institutions and Nonprofits only

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pCfB2989

Plasmid

#63636

Purpose

pCfB2989 is a vector for multiple integrations at sites sharing homology with Ty1Cons1. The selective marker is Kl.URA3. Additionally it contains a USER cassette.

Depositor

Irina Borodina, Jerome Maury

Article

Maury et al PLoS One. 2016 Mar 2;11(3):e0150394. doi

Type

Empty backbone

Use

Cre/Lox

Tags

Expression

Yeast

Mutation

Promoter

none

Available since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pCfB2802

Plasmid

#63655

Purpose

pCfB2802 is a vector for multiple integrations at sites sharing homology with Ty1Cons1. The selective marker is Kl.LEU2. Additionally it contains a PTEF1-GFP as reporter.

Depositor

Irina Borodina, Jerome Maury

Article

Maury et al PLoS One. 2016 Mar 2;11(3):e0150394. doi

Insert

GFP

Use

Cre/Lox

Tags

Expression

Yeast

Mutation

Promoter

TEF1 promoter

Available since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pCfB2990

Plasmid

#63645

Purpose

pCfB2990 is a vector for multiple integrations at sites sharing homology with Ty3Cons. The selective marker is Kl.LEU2. Additionally it contains a USER cassette.

Depositor

Irina Borodina, Jerome Maury

Article

Maury et al PLoS One. 2016 Mar 2;11(3):e0150394. doi

Type

Empty backbone

Use

Cre/Lox

Tags

Expression

Yeast

Mutation

Promoter

none

Available since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

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ZJOM12

Plasmid

#133638

Purpose

Integration of GFP-TLG2 as an additional copy in genome, use auxotrophic marker URA3(Saccharomyces cerevisiae). Marker for yeast late golgi/early endosome.

Depositor

Zhiping Xie

Article

Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

Insert

pCUP1-GFP-Tlg2 (TLG2 Budding Yeast)

Use

Tags

EGFP

Expression

Yeast

Mutation

Promoter

Available since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pCfB2800

Plasmid

#63657

Purpose

pCfB2800 is a vector for multiple integrations at sites sharing homology with Ty2Cons. The selective marker is Kl.LEU2. Additionally it contains a PTEF1-GFP as reporter.

Depositor

Irina Borodina, Jerome Maury

Article

Maury et al PLoS One. 2016 Mar 2;11(3):e0150394. doi

Insert

GFP

Use

Cre/Lox

Tags

Expression

Yeast

Mutation

Promoter

TEF1 promoter

Available since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pCfB2799

Plasmid

#63658

Purpose

pCfB2799 is a vector for multiple integrations at sites sharing homology with Ty3Cons. The selective marker is Kl.LEU2. Additionally it contains a PTEF1-GFP as reporter.

Depositor

Irina Borodina, Jerome Maury

Article

Maury et al PLoS One. 2016 Mar 2;11(3):e0150394. doi

Insert

GFP

Use

Cre/Lox

Tags

Expression

Yeast

Mutation

Promoter

TEF1 promoter

Available since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pCfB2992

Plasmid

#63642

Purpose

pCfB2992 is a vector for multiple integrations at sites sharing homology with Ty1Cons1. The selective marker is Kl.LEU2. Additionally it contains a USER cassette.

Depositor

Irina Borodina, Jerome Maury

Article

Maury et al PLoS One. 2016 Mar 2;11(3):e0150394. doi

Type

Empty backbone

Use

Cre/Lox

Tags

Expression

Yeast

Mutation

Promoter

none

Available since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
ZJOM8

Plasmid

#133671

Purpose

Integration of SNF7-GFP as an additional copy in genome, use auxotrophic marker URA3(Candida albicans). Present on endosomes and vacuolar surface.

Depositor

Zhiping Xie

Article

Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

Insert

p1k-SNF7-GFP (SNF7 Budding Yeast)

Use

Tags

EGFP

Expression

Yeast

Mutation

Promoter

Available since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pCfB2801

Plasmid

#63656

Purpose

pCfB2801 is a vector for multiple integrations at sites sharing homology with Ty1Cons2. The selective marker is Kl.LEU2. Additionally it contains a PTEF1-GFP as reporter.

Depositor

Irina Borodina, Jerome Maury

Article

Maury et al PLoS One. 2016 Mar 2;11(3):e0150394. doi

Insert

GFP

Use

Cre/Lox

Tags

Expression

Yeast

Mutation

Promoter

TEF1 promoter

Available since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pCK025

Plasmid

#105415

Purpose

Synthetic biology

Depositor

Johannes Stuttmann

Article

Gantner et al PLoS One. 2018 May 30;13(5):e0197185. do

Insert

Hormone-binding domain and regulatory sequences (estrogen receptor)

Use

Synthetic Biology

Tags

Expression

Mutation

BsaI/ BpiI restriction sites removed

Promoter

Available since

Aug. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pCfB2991

Plasmid

#63643

Purpose

pCfB2991 is a vector for multiple integrations at sites sharing homology with Ty1Cons2. The selective marker is Kl.LEU2. Additionally it contains a USER cassette.

Depositor

Irina Borodina, Jerome Maury

Article

Maury et al PLoS One. 2016 Mar 2;11(3):e0150394. doi

Type

Empty backbone

Use

Cre/Lox

Tags

Expression

Yeast

Mutation

Promoter

none

Available since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pDONR221 CARD8 FIIND

Plasmid

#169963

Purpose

pDONR221 for Gateway shuttling. Contains the FIIND (L162M-P434, no stop codon)

Depositor

Daniel Bachovchin

Article

Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

Insert

CARD8 (CARD8 Human)

Use

Gateway cloning vector

Tags

Expression

Mutation

Promoter

Available since

May 12, 2021

Availability

Academic Institutions and Nonprofits only

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pTEI037

Plasmid

#105413

Purpose

Synthetic biology

Depositor

Johannes Stuttmann

Article

Gantner et al PLoS One. 2018 May 30;13(5):e0197185. do

Insert

Hormone-binding domain (glucocorticoid receptor) fused to mEGFP

Use

Synthetic Biology

Tags

Expression

Mutation

BsaI/ BpiI restriction sites removed

Promoter

Available since

Aug. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pTEI041

Plasmid

#105414

Purpose

Synthetic biology

Depositor

Johannes Stuttmann

Article

Gantner et al PLoS One. 2018 May 30;13(5):e0197185. do

Insert

Hormone-binding domain and regulatory sequences (estrogen receptor)

Use

Synthetic Biology

Tags

Expression

Mutation

BsaI/ BpiI restriction sites removed

Promoter

Available since

Aug. 1, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pSF1389

Plasmid

#104962

Purpose

Bacterial expression plasmid of bdSENP1 protease

Depositor

Dirk Görlich

Article

Frey et al J Chromatogr A. 2014 Apr 11;1337:95-105.

Insert

SENP1

Use

Tags

His14 tag and Tev protease cleavage site

Expression

Bacterial

Mutation

Promoter

lacUV5

Available since

Jan. 24, 2018

Availability

Academic Institutions and Nonprofits only

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p4xEGFP-N1

Plasmid

#122164

Purpose

Expression of 4xEGFP

Depositor

Georg Mayr

Article

Bohm et al Anal Biochem. 2017 Sep 15;533:48-55. doi

Insert

4xEGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pG1AK-sfGFP

Plasmid

#71739

Purpose

Modular shuttle vector for Geobacillus and E. coli

Depositor

Tom Ellis

Article

Reeve et al ACS Synth Biol. 2016 Jul 1.

Insert

sfGFP

Use

Synthetic Biology; Shuttle vector

Tags

Expression

Bacterial

Mutation

Promoter

pRplsWT

Available since

July 5, 2016

Availability

Academic Institutions and Nonprofits only

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pECNUS_MP02

Plasmid

#194351

Purpose

CRISPR-Cas9 multiplexing acceptor clone with BsaI cutter

Depositor

Eunyoung Chae

Article

Lee et al Nucleic Acids Res. 2023 Mar 15:gkad142.

Insert

pcoCas9

Use

Tags

Expression

Plant

Mutation

Promoter

RPS5A

Available since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

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pFtnA-Halo

Plasmid

#98281

Purpose

mammalian expression of FtnA-Halo

Depositor

Mike Heilemann

Article

Finan et al Angew Chem Int Ed Engl. 2015 Oct 5;54(41

Insert

FtnA

Use

Tags

Halo-tag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 3, 2017

Availability

Academic Institutions and Nonprofits only

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