We narrowed to 1,231 results for: POLI

1. pET28_6xHis-PARP1-C125G

   Plasmid

   #174792

   Purpose

   Bacterial expression of a less active PARP1 mutant (C125G reduces affinity for single-strand DNA break)

   Depositor

   Joan Conaway

   Article

   Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

   Insert

   PARP1-C125G (PARP1 Human)
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   C125G, V762A

   Available Since

   Sept. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET28_6xHis-PARP1-Y986H

   Plasmid

   #174801

   Purpose

   Bacterial expression of a PARP1 mutant that produces highly branched but short PAR chains overall

   Depositor

   Joan Conaway

   Article

   Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

   Insert

   PARP1-Y986H (PARP1 Human)
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Y986H, V762A

   Available Since

   Sept. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pET28_6xHis-PARP1-Y986S

   Plasmid

   #174802

   Purpose

   Bacterial expression of a PARP1 mutant that produces mainly short PAR oligomers

   Depositor

   Joan Conaway

   Article

   Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

   Insert

   PARP1-Y986S (PARP1 Human)
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Y986H, V762A

   Available Since

   Sept. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pET28_6xHis-PARP1-G972R

   Plasmid

   #174800

   Purpose

   Bacterial expression of a PARP1 mutant that produces less PAR oligomers overall

   Depositor

   Joan Conaway

   Article

   Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

   Insert

   PARP1-G972R (PARP1 Human)
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   G972R, V762A

   Available Since

   Sept. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET28_6xHis-PARP1delWGR-L713F

   Plasmid

   #173941

   Purpose

   Bacterial expression of PARP1 lacking WGR domain but containing L713F gain-of-function mutation

   Depositor

   Joan Conaway

   Article

   Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

   Insert

   PARP1delWGR-L713F (PARP1 Human)
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Deletion of residues 521-660; L713F, V762A

   Available Since

   Sept. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET28_6xHis-PARP1-A774L

   Plasmid

   #174796

   Purpose

   Bacterial expression of a hyperactive PARP1 mutant (A774L destabilizes the autoinhibitory HD subdomain)

   Depositor

   Joan Conaway

   Article

   Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

   Insert

   PARP1-A774L (PARP1 Human)
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   A774L, V762A

   Available Since

   Sept. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pET28_6xHis-PARP1-W318R

   Plasmid

   #174793

   Purpose

   Bacterial expression of an inactive PARP1 (W318R disrupts interdomain communication and HD subdomain unfolding)

   Depositor

   Joan Conaway

   Article

   Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

   Insert

   PARP1-W318R (PARP1 Human)
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   W318, V762A

   Available Since

   Sept. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pET28_6xHis-PARP1-A762V

   Plasmid

   #174795

   Purpose

   Bacterial expression of wild type PARP1 mutant (A762V is reversion of the common V762A polymorphism)

   Depositor

   Joan Conaway

   Article

   Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

   Insert

   PARP1-A762V (PARP1 Human)
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   A762V

   Available Since

   Sept. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET28_6xHis-PARP1delZn1-L713F

   Plasmid

   #173937

   Purpose

   Bacterial expression of PARP1 lacking Zn1 domain but containing L713F gain-of-function mutation

   Depositor

   Joan Conaway

   Article

   Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

   Insert

   PARP1delZn1-L713F (PARP1 Human)
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Deletion of residues 1-96; L713F, V762A

   Available Since

   Sept. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET28_6xHis-PARP1delZn3-L713F

    Plasmid

    #173934

    Purpose

    Bacterial expression of PARP1 lacking Zn3 domain but containing L713F gain-of-function mutation

    Depositor

    Joan Conaway

    Article

    Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

    Insert

    PARP1delZn3-L713F (PARP1 Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Deletion of residues 214-373; L713F, V762A

    Available Since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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