We narrowed to 4,216 results for: Alk

1. pLIC-myc-GBAi W211A

   Plasmid

   #171754

   Purpose

   For the mammalian expression of myc-GBAi W211A

   Depositor

   Mikel Garcia-Marcos

   Article

   Leyme et al Proc Natl Acad Sci U S A. 2017 Nov 28;11

   Insert

   GBAi
   

   Use

   Tags

   myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. delCMV-mCherry-2x-Rhotekin-GBD

   Plasmid

   #214142

   Purpose

   Rho activity sensor

   Depositor

   Leif Dehmelt

   Article

   Nanda et al Nat Commun. 2023 Dec 15;14(1):8356. doi:

   Insert

   3xRhotekin-GBD (Rtkn Mouse)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Only the GTPase binding domain (GBD) is included

   Promoter

   delCMV

   Available since

   April 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGEX-5X-1-Full-Length-MePCE

   Plasmid

   #113539

   Purpose

   Produces an N-terminal GST-tagged full length MePCE protein.

   Depositor

   Blerta Xhemalce

   Article

   Shelton et al Cell Rep. 2018 Feb 6;22(6):1374-1383. do

   Insert

   MePCE (MEPCE Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcmv3-IRG1-HA

   Plasmid

   #198181

   Purpose

   Mammalian expression of HA-tagged human ACOD1 (IRG1)

   Depositor

   Yun Zhao

   Article

   Li et al Cell Rep. 2023 Mar 28;42(3):112145. doi:

   Insert

   ACOD1 (ACOD1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   HA tag is added to C-terminal of target protein

   Promoter

   CMV

   Available since

   April 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pscAluYNF1

   Plasmid

   #50934

   Purpose

   Expresses scAluY RNA in mammalian cells.

   Depositor

   Katharina Strub

   Article

   Ivanova et al Nucleic Acids Res. 2015 Mar 11;43(5):287

   Insert

   scAluYNF1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   the poly(A) linker and the right arm of the dimer…

   Promoter

   7SL RNA promoter (pol III)

   Available since

   May 16, 2014

   Availability

   Academic Institutions and Nonprofits only

6. pAAV.minBG-CI-SaCas9-W3SynpA

   Plasmid

   #231369

   Purpose

   minBG-driven SaCas9, with short synthetic terminator sequence.

   Depositor

   Viviana Gradinaru

   Article

   Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

   Insert

   SaCas9
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSC5

   Plasmid

   #188602

   Purpose

   Superior Conjugative Plasmid to Diverse Yeast

   Depositor

   Bogumil Karas

   Article

   Cochrane et al Biodes Res. 2022 Aug 19;2022:9802168. do

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available since

   April 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. His-SUMO-hDDI2_pET11a

   Plasmid

   #169120

   Purpose

   expression of protein

   Depositor

   Barbara Dirac-Svejstrup

   Article

   Dirac-Svejstrup et al Mol Cell. 2020 Jul 16;79(2):332-341.e7.

   Insert

   hDDI (DDI2 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pN565

   Plasmid

   #49990

   Purpose

   expresses an attenuated T7 RNAP under an isopropyl β-d-1-thiogalactopyranoside (IPTG) inducible control

   Depositor

   Christopher Voigt

   Article

   Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

   Insert

   T7 RNAP*
   

   Use

   Synthetic Biology

   Tags

   umuD degradation tag

   Expression

   Bacterial

   Mutation

   R632S

   Promoter

   pTac-symmetric

   Available since

   Jan. 31, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSC5GGv1

    Plasmid

    #194645

    Purpose

    pSC5 Golden-Gate Cloning Plasmid (BsaI - Site 1)

    Depositor

    Bogumil Karas

    Article

    Cochrane et al Biodes Res. 2022 Aug 19;2022:9802168. do

    Type

    Empty backbone

    Use

    Synthetic Biology; Diatom expression

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3 Ash2L full

    Plasmid

    #15548

    Depositor

    Kai Ge

    Article

    Cho et al J Biol Chem. 2007 Jul 13. 282(28):20395-

    Insert

    Ash2L (ASH2L Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    R622H (Please see depositor comments below)

    Promoter

    Available since

    Aug. 16, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEPQD0KN0750

    Plasmid

    #185627

    Purpose

    Modified tobacco rattle virus RNA2 acceptor vector that could be used for expression of sgRNAs, contains restriction enzyme sites for golden gate assembly using AarI

    Depositor

    Nicola Patron

    Article

    Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

    Insert

    lacZ
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. GFP-LiMETER-v1

    Plasmid

    #113933

    Purpose

    Express an optogenetic tether to label and interrogate ER-plasma membrane contact sites (MCS); visualized by GFP

    Depositor

    Yubin Zhou

    Article

    Jing et al Nat Cell Biol. 2015 Oct;17(10):1339-47.

    Insert

    LOV2 and Rit polybasic domain (PB)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. tet-pLKO shGATA6 puro

    Plasmid

    #72615

    Purpose

    Expresses an inducible short hairpin targeting human GATA6 sequence

    Depositor

    Kevin Janes

    Article

    Chitforoushzadeh et al Sci Signal. 2016 Jun 7;9(431):ra59. doi:

    Insert

    short hairpin GATA binding protein 6
    

    Use

    Lentiviral

    Tags

    None

    Expression

    Mammalian

    Mutation

    Promoter

    TRE Tight

    Available since

    Aug. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pmTurquoise2-LC3

    Plasmid

    #110947

    Purpose

    Expresses the autophagosome marker LC3 fused to mTurquoise2

    Depositor

    Pablo Rivera-Fuentes

    Article

    Nguyen et al Org Biomol Chem. 2021 Mar 28;19(12):2681

    Insert

    Microtubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)
    

    Use

    Tags

    mTurquoise2

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pVRa41_491

    Plasmid

    #49702

    Depositor

    Christopher Voigt

    Article

    Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

    Insert

    ECF41_491
    

    Use

    Synthetic Biology

    Tags

    His6-PreScission

    Expression

    Bacterial

    Mutation

    Codon optimized for E.coli

    Promoter

    pT7-lacO

    Available since

    Jan. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pVRa42_4454

    Plasmid

    #49703

    Depositor

    Christopher Voigt

    Article

    Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

    Insert

    ECF42_4454
    

    Use

    Synthetic Biology

    Tags

    His6-PreScission

    Expression

    Bacterial

    Mutation

    Codon optimized for E.coli

    Promoter

    pT7-lacO

    Available since

    Jan. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. miniABEMax V82G wt

    Plasmid

    #135362

    Purpose

    Expression of nSpyCas9 with N-terminal miniABEMax (V82G)

    Depositor

    Alex Hewitt

    Article

    Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

    Insert

    miniABEMax nSpyCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    (V82G)

    Promoter

    Available since

    Oct. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pECIA14

    Plasmid

    #47051

    Purpose

    This is a Gateway destination vector for secreted expression in Drosophila cell culture by CuSO4 induction. Inserts will be pentamerized and tagged with Alkaline phosphatase, FLAG and His tags

    Depositor

    Chris Garcia

    Article

    Ozkan et al Cell. 2013 Jul 3;154(1):228-39. doi: 10.

    Type

    Empty backbone

    Use

    Secreted expression in drosophila culture

    Tags

    Alkaline Phosphatase (human, placental), Flag, HR…

    Expression

    Insect

    Mutation

    Promoter

    Metallothionein (Copper-Inducible)

    Available since

    Aug. 30, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. pRK5 TGF beta type I receptor (T202D) Flag

    Plasmid

    #14833

    Depositor

    Rik Derynck

    Article

    Feng et al J Biol Chem. 1996 May 31. 271(22):13123-

    Insert

    TGF beta type I receptor (Tgfbr1 Rat)
    

    Use

    Tags

    flag

    Expression

    Mammalian

    Mutation

    Threonine 202 mutated to aspartate. Constitutivel…

    Promoter

    Available since

    May 15, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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