Addgene

We narrowed to 10,856 results for: kars;

Showing: 1301 - 1320 of 10856 results

Results per page:

Sort results by:

pOSIP-KH-RBS2-dCas9

Plasmid

#182740

Purpose

Integrative plasmid at the HK022 site in E. coli carrying dCas9 under the control of a Ptet promoter

Depositor

David Bikard

Article

Rousset et al PLoS Genet. 2018 Nov 7;14(11):e1007749.

Insert

dCas9

Use

CRISPR; Integrative vector

Tags

Expression

Mutation

Promoter

Ptet

Available since

May 16, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pLV-EF1a-MinE(L4E)-EGFP_IRES-Blast

Plasmid

#213117

Purpose

Expression of a faster-running MinE mutant in mammalian cells

Depositor

Scott Coyle

Article

Rajasekaran et al Cell. 2023 Dec 27:S0092-8674(23)01339-9.

Insert

MinE(L4E)-EGFP (minE Escherichia coli)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

L4E

Promoter

EF1a

Available since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pLV-EF1a-FRB-mCherry-MinD-IRES-Puro

Plasmid

#213116

Purpose

Expression of FRB-mCherry-MinD in mammalian cells for payload delivery

Depositor

Scott Coyle

Article

Rajasekaran et al Cell. 2023 Dec 27:S0092-8674(23)01339-9.

Insert

FRB-mCherry-MinD (minD Escherichia coli)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
AcpP pet29a C-terminal His Tag

Plasmid

#75016

Purpose

Expresses the acyl carrier protein (ACP) from the E. coli fatty acid synthase

Depositor

Michael Burkart

Article

Worthington et al ACS Chem Biol. 2006 Dec 20;1(11):687-91.

Insert

AcpP (acpP E. coli)

Use

Tags

His Tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 18, 2016

Availability

Academic Institutions and Nonprofits only
pUSE-RapR-Src(KD)-myc

Plasmid

#25934

Depositor

Klaus Hahn

Article

Karginov et al Nat Biotechnol. 2010 Jun 27. ():.

Insert

RapR-Src (Fkbp1a Mouse)

Use

Tags

Expression

Mammalian

Mutation

This pUSE-RapR-Src(KD)-myc plasmid contains the k…

Promoter

Available since

Oct. 26, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
CRT-GFP pcDNA3.1

Plasmid

#80978

Purpose

mammalian expression of CRT-GFP

Depositor

Peter Steyger

Article

Karasawa et al Toxicol Sci. 2011 Dec;124(2):378-87. doi

Insert

CRT (Calr Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

no KDEL

Promoter

CMV

Available since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only
PB-Antares2

Plasmid

#120868

Purpose

Piggybac transposon plasmid for live animal optical imaging

Depositor

Jordan Green

Article

Karlsson et al Adv Funct Mater. 2021 Apr 22;31(17). doi

Insert

pcDNA3-Antares2

Use

Tags

Expression

Mammalian

Mutation

Antares2 amplicon

Promoter

CAG Promoter

Available since

Feb. 14, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pLV-EF1a-PKAsub-mCherry-MinD-IRES-Puro

Plasmid

#213118

Purpose

Expression of one half of a PKAsub-mCherry-MinD reporter circuit in mammalian cells

Depositor

Scott Coyle

Article

Rajasekaran et al Cell. 2023 Dec 27:S0092-8674(23)01339-9.

Insert

PKAsub-mCherry-MinD (minD Escherichia coli)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pBabe neo TGFBR3-HA

Plasmid

#83095

Purpose

Retroviral vector for constitutive TGFBR3 expression with C-terminal HA tag

Depositor

Kevin Janes

Article

Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

Insert

TGFBR3 (TGFBR3 Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 28, 2019

Availability

Academic Institutions and Nonprofits only
pUC118-mCtnnb1-FLIP-FlpE-Puro

Plasmid

#84541

Purpose

CRISPR - FLIP-FlpE targeting vector for mouse beta-catenin

Depositor

Bon-Kyoung Koo

Article

Andersson-Rolf et al Nat Methods. 2017 Jan 30. doi: 10.1038/n

Insert

mouse beta-catenin FLIP-FlpE - Puro targeting vector

Use

Cre/Lox and Mouse Targeting

Tags

Expression

Mutation

Promoter

Available since

Jan. 31, 2017

Availability

Academic Institutions and Nonprofits only
pUC118-mCtnnb1-FLIP-Puro

Plasmid

#84540

Purpose

CRISPR - FLIP targeting vector for mouse beta-catenin

Depositor

Bon-Kyoung Koo

Article

Andersson-Rolf et al Nat Methods. 2017 Jan 30. doi: 10.1038/n

Insert

mouse beta-catenin FLIP - Puro targeting vector

Use

Cre/Lox and Mouse Targeting

Tags

Expression

Mutation

Promoter

Available since

Jan. 31, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA4-Myc-eIF3f

Plasmid

#35975

Depositor

Susana Valente

Article

Valente et al Proc Natl Acad Sci U S A. 2009 Mar 17;10

Insert

eukaryotic translation initiation factor 3, subunit F (EIF3F Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

Available since

April 9, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pAPcrRNA_Lmo

Plasmid

#126501

Purpose

L.monocytogenes CRISPR Type I-B crRNA cloning vector for mammalian expression driven by U6 promoter

Depositor

Charles Gersbach

Article

Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
RapR-Shp2-mVenus-flag

Plasmid

#188655

Purpose

Rapamycin regulated Shp2 phosphatase with mVenus and flag tag

Depositor

Andrei Karginov

Article

Fauser et al J Cell Biol. 2022 Aug 1;221(8). pii: 213

Insert

Shp2

Use

Tags

Flag and mVenus

Expression

Mammalian

Mutation

iFKBP insertion at V406 with deletion of residues…

Promoter

CMV

Available since

Aug. 19, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
TET-pLKO.1 PURO shSMAD4 #2

Plasmid

#83091

Purpose

Lentiviral shRNA vector for inducible knockdown of human SMAD4

Depositor

Kevin Janes

Article

Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

Insert

shSMAD4 (SMAD4 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 28, 2019

Availability

Academic Institutions and Nonprofits only
pLmoCascade-IL1RNcrRNA

Plasmid

#126487

Purpose

Encodes L.monocytogenes CRISPR Type I-B crRNA targeting IL1RN

Depositor

Charles Gersbach

Article

Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

Insert

Lmo IL1RN cr03

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
All-in-one split-ADAR2

Plasmid

#170136

Purpose

AAV vector carrying ADAR2-DDC-λN-P2A-MCP-ADAR2-DDN with a nuclear export signal

Depositor

Prashant Mali

Article

Katrekar et al Elife. 2022 Jan 19;11. pii: 75555. doi:

Insert

ADAR2-DDC-λN-P2A-MCP-ADAR2-DDN

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP-RapR-FAK-YM-KD

Plasmid

#25929

Depositor

Klaus Hahn

Article

Karginov et al Nat Biotechnol. 2010 Jun 27. ():.

Insert

RapR-FAK (Ptk2 Human, Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

This pEGFP-RapR-FAK-YM-KD plasmid contains the FA…

Promoter

Available since

Oct. 18, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
SiC-V1-Scr

Plasmid

#133042

Purpose

SiC-V1 vector with scramble (Scr) sgRNA. This lentivirus construct is used for stably expressing spCas9 with a dTomato reporter in a cell line of interest.

Depositor

Sriram Neelamegham

Article

Kelkar et al Mol Ther. 2019 Sep 12. pii: S1525-0016(1

Insert

SpCas9

Use

CRISPR and Lentiviral

Tags

dTomato

Expression

Mutation

Promoter

Available since

Nov. 26, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
BoxB-MS2 adRNA (RAB7A-ACG site)

Plasmid

#170155

Purpose

AAV vector carrying a BoxB-MS2 adRNA targeting the RAB7A transcript for C-U editing

Depositor

Prashant Mali

Article

Katrekar et al Elife. 2022 Jan 19;11. pii: 75555. doi:

Insert

BoxB-RAB7A(ACG)-MS2

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Human U6

Available since

March 8, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge