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pKG1055_pPV2-tagBFP

Plasmid

#239697

Purpose

BsaI Golden Gate assembly plasmid encoding the tagBFP CDS

Depositor

Kate Galloway

Article

Peterman et al Nucleic Acids Research, Volume 53, Issue

Insert

tagBFP

Use

Synthetic Biology

Mutation

Removal of BsaI cut sites

Available Since

July 10, 2025

Availability

Academic Institutions and Nonprofits only

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pKG0587_pPV3-bGH

Plasmid

#239701

Purpose

BsaI Golden Gate assembly plasmid encoding the bGH PAS

Depositor

Kate Galloway

Article

Peterman et al Nucleic Acids Research, Volume 53, Issue

Insert

bGH PAS

Use

Synthetic Biology

Available Since

July 10, 2025

Availability

Academic Institutions and Nonprofits only

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pKG0588_pPV3-SV40

Plasmid

#239702

Purpose

BsaI Golden Gate assembly plasmid encoding SV40 PAS

Depositor

Kate Galloway

Article

Peterman et al Nucleic Acids Research, Volume 53, Issue

Insert

SV40 PAS

Use

Synthetic Biology

Available Since

July 10, 2025

Availability

Academic Institutions and Nonprofits only

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pKG2039_pPV1-CAG

Plasmid

#239686

Purpose

BsaI Golden Gate assembly plasmid encoding the CAG promoter

Depositor

Kate Galloway

Article

Peterman et al Nucleic Acids Research, Volume 53, Issue

Insert

CAG promoter

Use

Synthetic Biology

Available Since

July 10, 2025

Availability

Academic Institutions and Nonprofits only

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pKG2019_pPV1-EF1a

Plasmid

#239687

Purpose

BsaI Golden Gate assembly plasmid encoding the EF1a promoter

Depositor

Kate Galloway

Article

Peterman et al Nucleic Acids Research, Volume 53, Issue

Insert

EF1a promoter

Use

Synthetic Biology

Available Since

July 10, 2025

Availability

Academic Institutions and Nonprofits only

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pAMC070n1a12

Plasmid

#209746

Purpose

ColA BBa_B0014 tetAp SD8 ScTrpRS rrnB1 proB SD8 Lum1PylRS BBaB10014RNAItrpA+ J23100 SD8 MmPylRS T3Te-T7Te DHFR

Depositor

Ahmed Badran

Article

Costello et al Nat Biotechnol. 2024 Sep 11. doi: 10.103

Insert

tRNA operon - ScTrpRS - Lum1PylRS - MmPylRS

Expression

Bacterial

Available Since

Oct. 18, 2024

Availability

Academic Institutions and Nonprofits only

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GB4650

Plasmid

#215265

Purpose

TU for the copper regulated expression of the PhiC31 recombinase.

Depositor

Diego Orzaez

Article

Calvache et al Plant Biotechnol J. 2024 Jan;22(1):37-47

Insert

CBS:miniDFR:PhiC31:tNos

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

July 26, 2024

Availability

Academic Institutions and Nonprofits only

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GB4652

Plasmid

#215266

Purpose

TU for the copper regulated expression of the flippase (FLP) recombinase.

Depositor

Diego Orzaez

Article

Calvache et al Plant Biotechnol J. 2024 Jan;22(1):37-47

Insert

CBS:miniDFR:FLP:TNOS

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

July 26, 2024

Availability

Academic Institutions and Nonprofits only

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GB1203

Plasmid

#215369

Purpose

TU for the expression of the silencing suppressor P19 driven by the 35s promoter

Depositor

Diego Orzaez

Article

Calvache et al Plant Biotechnol J. 2024 Jan;22(1):37-47

Insert

35s:P19:tNos

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

June 28, 2024

Availability

Academic Institutions and Nonprofits only

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pWR95(URA3)

Plasmid

#203917

Purpose

BLINCAR plasmid for gene deletion, URA3 target

Depositor

J. Brian Robertson

Article

Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:

Inserts

URA3 upstream homology
URA3 downstream homology

Expression

Yeast

Available Since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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pMC-1-ZmUbi1-6

Plasmid

#197714

Purpose

Phytobrick (MoClo) Level 0 Part

Depositor

Daniel Voytas

Article

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Insert

ZmUbi1 promoter

Expression

Plant

Available Since

Jan. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pMC-1-PvUbi1-6

Plasmid

#197705

Purpose

Phytobrick (MoClo) Level 0 Part

Depositor

Daniel Voytas

Article

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Insert

PvUbi1 promoter

Expression

Plant

Available Since

Jan. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pWR95

Plasmid

#203916

Purpose

BLINCAR plasmid for gene deletion, no genomic target

Depositor

J. Brian Robertson

Article

Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:

Insert

modified MCS

Expression

Yeast

Available Since

Oct. 4, 2023

Availability

Academic Institutions and Nonprofits only

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pJMC-3-Reverse

Plasmid

#197803

Purpose

Level 1 Module 3 destination vector (reverse orientation MoClo insert)

Depositor

Daniel Voytas

Article

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Type

Empty backbone

Use

Unspecified

Available Since

Aug. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pJMC-5-Esp3I

Plasmid

#197836

Purpose

Level 1 module 5 with Esp3I expansion point

Depositor

Daniel Voytas

Article

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Insert

None

Use

Unspecified

Available Since

Aug. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pJMC-5-Reverse

Plasmid

#197807

Purpose

Level 1 Module 5 destination vector (reverse orientation MoClo insert)

Depositor

Daniel Voytas

Article

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Type

Empty backbone

Use

Unspecified

Available Since

Aug. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pJMC-2T-Reverse

Plasmid

#197800

Purpose

Level 1 Module 2T destination vector (reverse orientation MoClo insert)

Depositor

Daniel Voytas

Article

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Type

Empty backbone

Use

Unspecified

Available Since

Aug. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pJMC-7T-Reverse

Plasmid

#197810

Purpose

Level 1 Module 7T destination vector (reverse orientation MoClo insert)

Depositor

Daniel Voytas

Article

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Type

Empty backbone

Use

Unspecified

Available Since

Aug. 16, 2023

Availability

Academic Institutions and Nonprofits only

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UBE2G2 shRNA

Plasmid

#201813

Purpose

siRNA

Depositor

Allan Weissman

Article

Chen et al Proc Natl Acad Sci U S A. 2006 Jan 10. 1

Insert

UBE2G2 (UBE2G2 Human)

Use

RNAi

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pMC-6-Integrase13-9

Plasmid

#197728

Purpose

Phytobrick (MoClo) Level 0 Part

Depositor

Daniel Voytas

Article

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Insert

Integrase13 (tomato optimized)

Expression

Plant

Available Since

June 14, 2023

Availability

Academic Institutions and Nonprofits only

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