We narrowed to 27,003 results for: tat

1. BS-ZJLEU-PTEF1-HFL1-I375A-GFP

   Plasmid

   #207020

   Purpose

   Expression of Hfl1 point mutant. Uses auxotrophic marker LEU2 (Saccharomyces cerevisiae).

   Depositor

   Zhiping Xie

   Article

   He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

   Insert

   HFL1
   

   Use

   Tags

   GFP

   Expression

   Yeast

   Mutation

   I375A

   Promoter

   pTEF1

   Available since

   June 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. BS-ZJLEU-PTEF1-HFL1-W371A-GFP

   Plasmid

   #207019

   Purpose

   Expression of Hfl1 point mutant. Uses auxotrophic marker LEU2 (Saccharomyces cerevisiae).

   Depositor

   Zhiping Xie

   Article

   He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

   Insert

   HFL1
   

   Use

   Tags

   GFP

   Expression

   Yeast

   Mutation

   W371A

   Promoter

   pTEF1

   Available since

   June 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. BS-TRP1-TetOff(7repeats)-GFP-Pho8

   Plasmid

   #207011

   Purpose

   Tet-Off controlled GFP-Pho8 with TRP1 selection. Tetracycline-controlled transactivator (tTA) present on same plasmid.

   Depositor

   Zhiping Xie

   Article

   He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

   Insert

   Pho8
   

   Use

   Tags

   GFP

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. Ypq2-2GFP-URA

   Plasmid

   #207010

   Purpose

   Integration of 2xGFP tag at YPQ2 C terminus, use auxotrophic marker URA3(Kluyveromyces lactis) for selection. Marker for yeast vacuolar membrane.

   Depositor

   Zhiping Xie

   Article

   He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

   Insert

   YPQ2
   

   Use

   Tags

   2xGFP

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. Vph1-2GFP-URA

   Plasmid

   #207009

   Purpose

   Integration of 2xGFP tag at VPH1 C terminus, use auxotrophic marker URA3(Kluyveromyces lactis) for selection. Marker for yeast vacuolar membrane.

   Depositor

   Zhiping Xie

   Article

   He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

   Insert

   VPH1
   

   Use

   Tags

   2xGFP

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. p4A8_M59I_T94M_BC

   Plasmid

   #217833

   Purpose

   4A8 variant in barcoded, yeast surface display plasmid

   Depositor

   Timothy Whitehead

   Article

   Petersen et al Nat Commun. 2024 May 10;15(1):3974. doi:

   Insert

   4A8 variant Fab
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   VH: M59I, VL: T94M

   Promoter

   Available since

   April 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBDP

   Plasmid

   #217827

   Purpose

   Stuffer plasmid with golden gate compatible bidirectional promoter

   Depositor

   Timothy Whitehead

   Article

   Petersen et al Nat Commun. 2024 May 10;15(1):3974. doi:

   Insert

   Gal1/Gal10 Bidirectional Promoter
   

   Use

   Cloning vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. ClhN-P1K-3VSV-Atg8-MET15

   Plasmid

   #207055

   Purpose

   Expression of 3VSV-Atg8. Uses auxotrophic marker MET15(Saccharomyces cerevisiae).

   Depositor

   Zhiping Xie

   Article

   He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

   Insert

   ATG8
   

   Use

   Tags

   3xVSV

   Expression

   Yeast

   Mutation

   Promoter

   pATG8

   Available since

   March 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. P1K-3HA-Atg8∆R-MET15

   Plasmid

   #207034

   Purpose

   Expression of 3HA-Atg8∆R. This variant bypasses the need of Atg4 for initial C-terminal processing.

   Depositor

   Zhiping Xie

   Article

   He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

   Insert

   ATG8
   

   Use

   Tags

   3xHA

   Expression

   Yeast

   Mutation

   ∆R

   Promoter

   pATG8

   Available since

   Feb. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCRISPR2_IRdist_scram

    Plasmid

    #162319

    Purpose

    P. aeruginosa PA14 CRISPR2 locus, with a scrambled distal Inverted Repeat of the upstream motif

    Depositor

    Blake Wiedenheft

    Article

    Santiago-Frangos et al Curr Biol. 2021 Aug 23;31(16):3515-3524.

    Insert

    Pseudomonas aeruginosa PA14 CRISPR2 locus
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    The distal Upstream Motif site is scrambled

    Promoter

    pBAD

    Available since

    Dec. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. 185_pETcon_SARS2_BQ1-1

    Plasmid

    #208085

    Purpose

    yeast surface display of the SARS-CoV-2 BQ.1.1 variant RBD

    Depositor

    Tyler Starr

    Article

    Taylor et al PLoS Pathog. 2023 Dec 29;19(12):e1011901

    Insert

    SARS-CoV-2 BQ.1.1 RBD (S Budding Yeast, SARS-CoV-2 virus)
    

    Use

    Tags

    HA and c-Myc

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBEL1954

    Plasmid

    #195689

    Purpose

    Expression/purification of 6xHis-TEV-MlaD(F118A/L123A)

    Depositor

    Gira Bhabha, Damian Ekiert

    Article

    MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

    Insert

    MlaD
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    6xHis-TEV-MlaD(F118A/L123A)

    Promoter

    Available since

    May 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBEL2319

    Plasmid

    #195697

    Purpose

    Cryo-EM of 6xHis-Foldon-(Gly-Ser-Linker)-MlaC

    Depositor

    Gira Bhabha, Damian Ekiert

    Article

    MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

    Insert

    Foldon-MlaC
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    6xHis-Foldon-(Gly-Ser-Linker)-MlaC

    Promoter

    Available since

    May 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pBEL2707

    Plasmid

    #195636

    Purpose

    Expression/purification of 6xHis-TEV-sfGFP-MlaA (227-251)

    Depositor

    Gira Bhabha, Damian Ekiert

    Article

    MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

    Insert

    sfGFP-MlaA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    6xHis-TEV-sfGFP-MlaA (227-251)

    Promoter

    Available since

    May 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pBEL2753

    Plasmid

    #195637

    Purpose

    Expression/purification of 6xHis-TEV-sfGFP-MlaA (227-251; I241N)

    Depositor

    Gira Bhabha, Damian Ekiert

    Article

    MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

    Insert

    sfGFP-MlaA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    6xHis-TEV-sfGFP-MlaA (227-251; I241N)

    Promoter

    Available since

    May 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pBEL2754

    Plasmid

    #195638

    Purpose

    Expression/purification of 6xHis-TEV-sfGFP-MlaA (227-251; L245N)

    Depositor

    Gira Bhabha, Damian Ekiert

    Article

    MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

    Insert

    sfGFP-MlaA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    6xHis-TEV-sfGFP-MlaA (227-251; L245N)

    Promoter

    Available since

    May 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pBEL2755

    Plasmid

    #195639

    Purpose

    Expression/purification of 6xHis-TEV-sfGFP-MlaA (227-251; I248N)

    Depositor

    Gira Bhabha, Damian Ekiert

    Article

    MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

    Insert

    sfGFP-MlaA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    6xHis-TEV-sfGFP-MlaA (227-251; I248N)

    Promoter

    Available since

    May 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pBEL2756

    Plasmid

    #195640

    Purpose

    Expression/purification of 6xHis-TEV-sfGFP-MlaA(227-251; delta238-251)

    Depositor

    Gira Bhabha, Damian Ekiert

    Article

    MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

    Insert

    sfGFP-MlaA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    6xHis-TEV-sfGFP-MlaA(227-251; delta238-251)

    Promoter

    Available since

    May 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pBEL2757

    Plasmid

    #195641

    Purpose

    Expression/purification of 6xHis-TEV-sfGFP-MlaA(227-251; delta244-251)

    Depositor

    Gira Bhabha, Damian Ekiert

    Article

    MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

    Insert

    sfGFP-MlaA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    6xHis-TEV-sfGFP-MlaA(227-251; delta244-251)

    Promoter

    Available since

    May 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. H1.224.M.luc

    Plasmid

    #193705

    Purpose

    Expresses a truncated PolIII H1 promoter of 224 nucleotides (H1.224) with the TATA box mutated

    Depositor

    Elena Herrera-Carrillo

    Article

    Gao et al J Biol Chem. 2020 Nov 5. pii: S0021-9258

    Insert

    Truncated PolIII H1 promoter (H1.224) with the TATA box mutated
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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