We narrowed to 1,919 results for: Flp

Showing: 1321 - 1340 of 1919 results

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CIAR_pcDNA5/FRT/TO

Plasmid

#86502

Purpose

Expresses CIAR in mammalian cells. Used to generate Flp-In stables.

Depositor

Dustin Maly

Article

Rose et al Nat Chem Biol. 2016 Nov 21. doi: 10.1038

Insert

CIAR (SOS1 Human)

Use

Frt

Expression

Mammalian

Mutation

T968L in SOScat

Promoter

CMV (tet operator)

Available Since

Feb. 8, 2017

Availability

Academic Institutions and Nonprofits only

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CMV-218_HNF4A2

Plasmid

#31090

Depositor

Gerhart Ryffel

Article

Senkel et al BMC Res Notes. 2009 Oct 17;2:210. doi: 1

Insert

HNF4A (HNF4A Human)

Use

Flp/frt

Tags

myc

Expression

Mammalian

Mutation

splice variant 2 of HNF4A, deletion of CMV promo…

Available Since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only

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CMV-138_HNF4A8

Plasmid

#31093

Depositor

Gerhart Ryffel

Article

Senkel et al BMC Res Notes. 2009 Oct 17;2:210. doi: 1

Insert

HNF4A (HNF4A Human)

Use

Flp/frt

Tags

myc

Expression

Mammalian

Mutation

splice variant 8 of HNF4A, deletion of CMV promo…

Available Since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only

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CMV-68_HNF4A2

Plasmid

#31092

Depositor

Gerhart Ryffel

Article

Senkel et al BMC Res Notes. 2009 Oct 17;2:210. doi: 1

Insert

HNF4A (HNF4A Human)

Use

Flp/frt

Tags

myc

Expression

Mammalian

Mutation

splice variant 2 of HNF4A, deletion of CMV promo…

Available Since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only

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pLCMV:ECFP(loxP)(FRT)HNF1B

Plasmid

#31439

Depositor

Gerhart Ryffel

Article

Sauert et al PLoS One. 2012;7(3):e33522. Epub 2012 Ma

Insert

HNF1B (HNF1B Human)

Use

Cre/Lox; Flp/frt

Tags

Myc

Expression

Mammalian

Available Since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only

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FR_HNF1B_A263insGG

Plasmid

#31103

Depositor

Gerhart Ryffel

Article

Thomas et al Nucleic Acids Res. 2004 . 32(19):e150.

Insert

HNF1B (HNF1B Human)

Use

Flp/frt

Tags

myc

Expression

Mammalian

Mutation

mutation A263insGG

Available Since

Oct. 17, 2011

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO/SH/GW-Mst2

Plasmid

#84300

Purpose

Expresses a tetracycline-inducible SH-tagged (streptavidin-binding peptide and HA tag) version of Mst2. Used in conjunction with Flp-In cells to generate single copy transgene.

Depositor

Jonathan Chernoff

Article

Rawat et al J Biol Chem. 2013 Mar 22;288(12):8762-71

Insert

Mst2 (STK3 Human)

Tags

HA, SBP

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
FR_HNF1B_P328L329del

Plasmid

#31102

Depositor

Gerhart Ryffel

Article

Thomas et al Nucleic Acids Res. 2004 . 32(19):e150.

Insert

HNF1B (HNF1B Human)

Use

Flp/frt

Tags

myc

Expression

Mammalian

Mutation

mutation P328L329delCCTCT

Available Since

Oct. 28, 2011

Availability

Academic Institutions and Nonprofits only

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pSF3-Flag-Cbir-Cdc25C S216A_Myc-Nbir-14-3-3e

Plasmid

#90011

Purpose

Split-BioID plasmid: Expresses N-terminally tagged CBir[257-321]-Cdc25C S216A and N-terminally tagged NBir[2-256]-14-3-3e

Depositor

Julien Béthune

Article

Schopp et al Nat Commun. 2017 Jun 6;8:15690. doi: 10.

Inserts

CBirA*-Cdc25C S216A
NBirA*-14-3-3e

Use

Retroviral; Flp/frt

Tags

FLAG and Myc

Expression

Mammalian

Mutation

Ser 216 to Ala

Promoter

CMV

Available Since

Jan. 2, 2018

Availability

Academic Institutions and Nonprofits only

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CMV-138_HNF4A2

Plasmid

#31091

Depositor

Gerhart Ryffel

Article

Senkel et al BMC Res Notes. 2009 Oct 17;2:210. doi: 1

Insert

HNF4A (HNF4A Human)

Use

Flp/frt

Tags

myc

Expression

Mammalian

Mutation

splice variant 2 of HNF4A, deletion of CMV promo…

Available Since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only

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pLCMV:ECFP(loxP)(FRT)HNF1B_P328L329del

Plasmid

#30528

Depositor

Gerhart Ryffel

Article

Roose et al Transgenic Res. 2009 Aug . 18(4):595-605

Insert

HNF1B (HNF1B Human)

Use

Cre/Lox; Flp/frt

Tags

Myc

Expression

Mammalian

Mutation

P328L329delCCTCT

Available Since

Oct. 28, 2011

Availability

Academic Institutions and Nonprofits only

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pLCMV:ECFP(loxP)(FRT)HNF1B_A263ins

Plasmid

#31440

Depositor

Gerhart Ryffel

Article

Sauert et al PLoS One. 2012;7(3):e33522. Epub 2012 Ma

Insert

HNF1B (HNF1B Human)

Use

Cre/Lox; Flp/frt

Tags

Myc

Expression

Mammalian

Mutation

A263insGG

Available Since

Oct. 28, 2011

Availability

Academic Institutions and Nonprofits only

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P2_DD-HNF4A8

Plasmid

#31095

Depositor

Gerhart Ryffel

Article

Senkel et al BMC Res Notes. 2009 Oct 17;2:210. doi: 1

Insert

HNF4A (HNF4A Human)

Use

Flp/frt

Tags

FKBP L106P and myc

Expression

Mammalian

Mutation

splice variant 8 derived from promoter P2

Available Since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only

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xCIAR_pcDNA5/FRT/TO

Plasmid

#86504

Purpose

Expresses inactive control CIAR (xCIAR) in mammalian cells. Used to generate Flp-In stables.

Depositor

Dustin Maly

Article

Rose et al Nat Chem Biol. 2016 Nov 21. doi: 10.1038

Insert

xCIAR (SOS1 Human)

Use

Frt

Expression

Mammalian

Mutation

F929A and T968L in SOScat

Promoter

CMV (tet operator)

Available Since

Feb. 8, 2017

Availability

Academic Institutions and Nonprofits only

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P2_DD-HNF4A8_C106R

Plasmid

#31096

Depositor

Gerhart Ryffel

Article

Senkel et al BMC Res Notes. 2009 Oct 17;2:210. doi: 1

Insert

HNF4A (HNF4A Human)

Use

Flp/frt

Tags

FKBP L106P and myc

Expression

Mammalian

Mutation

splice variant 8 derived from promoter P2 with zi…

Available Since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only

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pV1524

Plasmid

#111431

Purpose

CaCas9/gRNA Solo entry plasmid for cloning guides - contains stuffer with BsmBI sites - inserts at Neut5L - Recyclable for serial mutagenesis (Mal2-FLP)

Depositor

Gerald Fink

Article

Vyas et al mSphere. 2018 Apr 25;3(2). pii: 3/2/e001

Insert

CaCas9/sgRNA-BsmBI stuffer

Use

CRISPR

Expression

Yeast

Available Since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pV1393

Plasmid

#111430

Purpose

CaCas9/gRNA Solo entry plasmid for cloning guides - contains stuffer with BsmBI sites - inserts at Neut5L - Recyclable for serial mutagenesis (Sap2-FLP)

Depositor

Gerald Fink

Article

Vyas et al mSphere. 2018 Apr 25;3(2). pii: 3/2/e001

Insert

CaCas9/sgRNA-BsmBI stuffer

Use

CRISPR

Expression

Yeast

Available Since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pSF3-Flag-CBir-FRB_Myc-NBir-FKBP

Plasmid

#90003

Purpose

Split-BioID plasmid: Expresses N-terminally tagged CBir[257-321]-FRB and N-terminally tagged NBir[2-256]-FKBP

Depositor

Julien Béthune

Article

Schopp et al Nat Commun. 2017 Jun 6;8:15690. doi: 10.

Inserts

NBirA*-linker-FKBP
CBirA*-linker-FRB

Use

Retroviral; Flp/frt

Tags

FLAG and Myc

Expression

Mammalian

Promoter

CMV

Available Since

July 5, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-fDIO-mNeonGreen

Plasmid

#99133

Purpose

An AAV genome with Flp recombinase-dependent expression of mNeonGreen from the CAG promoter

Depositor

Viviana Gradinaru

Article

Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

Has Service

AAV1

Insert

fDIO-mNeonGreen

Use

AAV

Expression

Mammalian

Promoter

CAG

Available Since

March 15, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-FLEXFRT-ChR2(H134R)-mCherry

Plasmid

#75470

Purpose

Flp-dependent Chr2-mCherry encoded in an AAV vector for rAAV production and expression in mammalian cells

Depositor

Connie Cepko

Article

Tang et al Elife. 2016 May 20;5. pii: e15312. doi:

Has Service

AAV1

Insert

Reversed ChR2(H134R)-mCherry

Use

AAV and Synthetic Biology

Expression

Mammalian

Promoter

CAG

Available Since

July 22, 2016

Availability

Academic Institutions and Nonprofits only

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