We narrowed to 2,728 results for: GUS

1. pAAV Syn hBE Rh Guanylyl Cyclase1 2A tDimer

   Plasmid

   #66779

   Purpose

   The rhodopsin-guanylyl cyclase of the aquatic fungus Blastocladiella emersonii enables fast optical control of cGMP signaling

   Depositor

   Thomas Oertner

   Article

   Scheib et al Sci Signal. 2015 Aug 11;8(389):rs8. doi:

   Inserts

   hbeRhGC
   red fluorescent protein
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   human Synapsin

   Available since

   Oct. 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCytERM-mGreenLantern

   Plasmid

   #164467

   Purpose

   Visualization of the ER in cells (compatible with OSER assay)

   Depositor

   Gregory Petsko

   Article

   Campbell et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

   Insert

   mGreenLantern
   

   Use

   Tags

   Cytochrome p450 first 29 animo acids (Oryctolagus…

   Expression

   Mammalian

   Mutation

   Clover-F64L/S72A/E124V/N149K/I167T/S175G/A206K/L2…

   Promoter

   CMV

   Available since

   Jan. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLKO.1puro-shWillin-B

   Plasmid

   #40886

   Depositor

   Paul Reynolds

   Article

   Angus et al Oncogene. 2012 Jan 12;31(2):238-50. doi:

   Insert

   shWillin-B (FRMD6 Human)
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 5, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLKO.1puro-shWillin-A

   Plasmid

   #40885

   Depositor

   Paul Reynolds

   Article

   Angus et al Oncogene. 2012 Jan 12;31(2):238-50. doi:

   Insert

   shWillin-A (FRMD6 Human)
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 5, 2012

   Availability

   Academic Institutions and Nonprofits only

5. ER-RspA-NFAST

   Plasmid

   #233595

   Purpose

   Expression of RspA-NFAST on the ER membrane

   Depositor

   Riccardo Filadi

   Article

   Garcia Casas et al Nat Commun. 2024 Nov 12;15(1):9775. doi:

   Insert

   RspA-NFAST
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pPB-EF1A-Puro-mCherry-RAB7-S72A

   Plasmid

   #221556

   Purpose

   Expresses Cherry-tagged human Rab7 with S72A mutation in mammalian cells. Compatible with piggybac transposase for genome integration.

   Depositor

   Shawn Ferguson

   Article

   Talaia et al bioRxiv [Preprint]. 2023 Dec 17:2023.12.

   Insert

   Rab7A (RAB7A Human)
   

   Use

   Piggybac

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Change serine 72 to alanine

   Promoter

   EF1a

   Available since

   July 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSpCas9-2A-Puro-RAB7A-gRNA2 (PX459)

   Plasmid

   #221552

   Purpose

   Expresses Cas9 and gRNA for disruption of Rab7A gene in human cells

   Depositor

   Shawn Ferguson

   Article

   Talaia et al bioRxiv [Preprint]. 2023 Dec 17:2023.12.

   Insert

   gRNA targeting human Rab7A (RAB7A Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   July 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. hSyn-DIO-somBiPOLES-mCerulean (AAV Retrograde)

   Viral Prep

   #154951-AAVrg

   Purpose

   Ready-to-use AAV Retrograde particles produced from hSyn-DIO-somBiPOLES-mCerulean (#154951). In addition to the viral particles, you will also receive purified hSyn-DIO-somBiPOLES-mCerulean plasmid DNA. Synapsin-driven, Cre-dependent expression of soma-targeted BiPOLES for optogenetic inhibition (blue light) and activation (red light). These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Simon Wiegert

   Article

   Vierock et al Nat Commun. 2021 Jul 26;12(1):4527. doi:

   Promoter

   Syn

   Tags

   mCerulean (Cre-dependent)

   Available since

   Sept. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

9. pSCAR_sgRNA_puro-mKate-lox2272

   Plasmid

   #162076

   Purpose

   3rd generation lentivirus sgRNA delivery backbone with Cre-removable mKate2 reporter and puromycin resistance

   Depositor

   Robert Manguso

   Article

   Dubrot et al Immunity. 2021 Jan 20. pii: S1074-7613(2

   Type

   Empty backbone

   Use

   CRISPR, Cre/Lox, and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6, EF1a

   Available since

   Feb. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAG-Beatrix

    Plasmid

    #167528

    Purpose

    Dual-color fluorescent reporter for the creation and detection of Cre-dependent tunable mosaics in floxed mouse lines and cells.

    Depositor

    Gian Michele Ratto

    Article

    Trovato et al Nat Commun. 2020 Dec 3;11(1):6194. doi:

    Insert

    Beatrix
    

    Use

    Cre/Lox and Mouse Targeting

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    April 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSCAR_sgRNA_hygro-tagBFP-lox5171

    Plasmid

    #162070

    Purpose

    3rd generation lentivirus sgRNA delivery backbone with Cre-removable tagBFP reporter and hygromycin resistance

    Depositor

    Robert Manguso

    Article

    Dubrot et al Immunity. 2021 Jan 20. pii: S1074-7613(2

    Type

    Empty backbone

    Use

    CRISPR, Cre/Lox, and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6, EF1a

    Available since

    Feb. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSCAR_sgRNA_blast-tagBFP-lox5171

    Plasmid

    #162072

    Purpose

    3rd generation lentivirus sgRNA delivery backbone with Cre-removable tagBFP reporter and blasticidin resistance

    Depositor

    Robert Manguso

    Article

    Dubrot et al Immunity. 2021 Jan 20. pii: S1074-7613(2

    Type

    Empty backbone

    Use

    CRISPR, Cre/Lox, and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6, EF1a

    Available since

    Feb. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pSCAR_sgRNA_hygro-tagBFP-lox2272

    Plasmid

    #162078

    Purpose

    3rd generation lentivirus sgRNA delivery backbone with Cre-removable tagBFP reporter and hygromycin resistance

    Depositor

    Robert Manguso

    Article

    Dubrot et al Immunity. 2021 Jan 20. pii: S1074-7613(2

    Type

    Empty backbone

    Use

    CRISPR, Cre/Lox, and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6, EF1a

    Available since

    Feb. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pSCAR_sgRNA_blast-tagBFP-lox2272

    Plasmid

    #162071

    Purpose

    3rd generation lentivirus sgRNA delivery backbone with Cre-removable tagBFP reporter and blasticidin resistance

    Depositor

    Robert Manguso

    Article

    Dubrot et al Immunity. 2021 Jan 20. pii: S1074-7613(2

    Type

    Empty backbone

    Use

    CRISPR, Cre/Lox, and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6, EF1a

    Available since

    Feb. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pIRESh-GFPII-SM-Dlk1

    Plasmid

    #108932

    Purpose

    GFP-tagged vector expressing full length isoform of Dlk1

    Depositor

    Anne Ferguson-Smith

    Article

    Ferron et al Nature. 2011 Jul 20;475(7356):381-5. doi

    Insert

    Dlk1 (Dlk1 Mouse)
    

    Use

    Tags

    GFPII

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSCAR_sgRNA_puro-mKate-lox5171

    Plasmid

    #162077

    Purpose

    3rd generation lentivirus sgRNA delivery backbone with Cre-removable mKate2 reporter and puromycin resistance

    Depositor

    Robert Manguso

    Article

    Dubrot et al Immunity. 2021 Jan 20. pii: S1074-7613(2

    Type

    Empty backbone

    Use

    CRISPR, Cre/Lox, and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6, EF1a

    Available since

    Feb. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLX_EFS-Cre_ppt-del

    Plasmid

    #162073

    Purpose

    Compact Cre expression vector with modified PPT designed to generate high-titer non-integrating lentivirus when packaged with PsPax2-D64V (Addgene #63586)

    Depositor

    Robert Manguso

    Article

    Dubrot et al Immunity. 2021 Jan 20. pii: S1074-7613(2

    Insert

    Cre
    

    Use

    Cre/Lox and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    codon-optimized for expression in M. musculus

    Promoter

    EFS (part of insert)

    Available since

    Feb. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSCAR_Cas9-blast_GFP

    Plasmid

    #162074

    Purpose

    3rd generation lentivirus transfer vector for Cre-removable Cas9, GFP reporter, and blast resistance

    Depositor

    Robert Manguso

    Article

    Dubrot et al Immunity. 2021 Jan 20. pii: S1074-7613(2

    Inserts

    Cas9-2a-blasticidin S deaminase
    EGFP
    

    Use

    CRISPR, Cre/Lox, and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    codon-optimized for expression in M. musculus

    Promoter

    EF1a and sv40

    Available since

    Feb. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSCAR_Cas9-hygro_GFP

    Plasmid

    #162075

    Purpose

    3rd generation lentivirus transfer vector for Cre-removable Cas9, GFP reporter, and hygro resistance

    Depositor

    Robert Manguso

    Article

    Dubrot et al Immunity. 2021 Jan 20. pii: S1074-7613(2

    Inserts

    Cas9-2a-hygromycin B phosphotransferase
    EGFP
    

    Use

    CRISPR, Cre/Lox, and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    codon-optimized for expression in M. musculus

    Promoter

    EF1a and sv40

    Available since

    Feb. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pIRESh-GFPII-MB-Dlk1

    Plasmid

    #108930

    Purpose

    GFP-tagged vector expressing membrane-bound Dlk1

    Depositor

    Anne Ferguson-Smith

    Article

    Ferron et al Nature. 2011 Jul 20;475(7356):381-5. doi

    Insert

    Dlk1 (Dlk1 Mouse)
    

    Use

    Tags

    GFPII

    Expression

    Mammalian

    Mutation

    lacks coding sequence for the juxtamembrane motif…

    Promoter

    Available since

    May 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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