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pDK197

Plasmid

#134189

Purpose

Retroviral plasmid expressing LAP-Clasp C terminus

Depositor

Iain Cheeseman

Article

Kern et al J Cell Biol. 2016 May 9;213(3):315-28. d

Insert

LAP-Clasp C terminus (CLASP1 Human)

Use

Retroviral

Tags

LAP (GFP)

Expression

Mammalian

Available Since

Feb. 21, 2020

Availability

Academic Institutions and Nonprofits only

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GST-SEPT5 WT

Plasmid

#27269

Depositor

Harish Pant

Article

Amin et al J Neurosci. 2008 Apr 2. 28(14):3631-43.

Insert

Septin 5 (SEPTIN5 Human)

Tags

GST

Expression

Bacterial

Available Since

Jan. 5, 2011

Availability

Academic Institutions and Nonprofits only
SEPT5 Mut(S327A)

Plasmid

#27274

Depositor

Harish Pant

Article

Amin et al J Neurosci. 2008 Apr 2. 28(14):3631-43.

Insert

Septin 5 (SEPTIN5 Human)

Tags

Myc (MASMQKLISEEDL)

Expression

Mammalian

Mutation

S327A

Available Since

Jan. 27, 2011

Availability

Academic Institutions and Nonprofits only
pSEM397 - mosTI unc-119 - NNN - operon - gfp - nls - tbb-2

Plasmid

#182354

Purpose

Nuclear GFP co-expression cloning vector for mosTI(unc-119). Transgene inserted upstream of gpd-2 operon::gfp(2xNLS)::tbb-2 3'UTR. MCS or Golden Gate (BsaI: tgcc - MCS - tgag)

Depositor

Christian Froekjaer-Jensen

Article

El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

Type

Empty backbone

Expression

Worm

Available Since

May 13, 2022

Availability

Academic Institutions and Nonprofits only

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pSEM399 - mosTI unc-119 - NNN - operon - mScarlet - nls - tbb-2

Plasmid

#182356

Purpose

Nuclear mScarlet co-expression cloning vector for mosTI(unc-119). Transgene inserted upstream of gpd-2 operon::mScarlet(2xNLS)::tbb-2 3'UTR. MCS or Golden Gate (BsaI: tgcc - MCS - tgag)

Depositor

Christian Froekjaer-Jensen

Article

El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

Type

Empty backbone

Expression

Worm

Available Since

May 13, 2022

Availability

Academic Institutions and Nonprofits only

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TRIN-E-shTAF12.376

Plasmid

#105573

Purpose

Dox-inducible mirE TAF12 shRNA/dsRED expression with GFP marker and neo resistance

Depositor

Christopher Vakoc

Article

Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

Insert

TAF12 shRNA

Use

RNAi and Retroviral

Expression

Mammalian

Promoter

TRE

Available Since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only
pAP2050-2

Plasmid

#105243

Purpose

mammalian GFP11 with linker::TEV::3xFlag::Ollas::Myc::V5::HAtag::other linker

Depositor

Geraldine Seydoux

Article

Paix et al Proc Natl Acad Sci U S A. 2017 Nov 28. p

Insert

GFP11 with extra-sequence (GFP11 with linker::TEV::3xFlag::Ollas::Myc::V5::HAtag::other linker)

Expression

Bacterial

Available Since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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GST-SEPT5 S327A

Plasmid

#27271

Depositor

Harish Pant

Article

Amin et al J Neurosci. 2008 Apr 2. 28(14):3631-43.

Insert

Septin 5 (SEPTIN5 Human)

Tags

GST

Expression

Bacterial

Mutation

S327A

Available Since

Jan. 25, 2011

Availability

Academic Institutions and Nonprofits only
PCDNA3-TAF4(827-909)

Plasmid

#105614

Purpose

transient express TAF4 HFD in HEK 293T cells

Depositor

Christopher Vakoc

Article

Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

Insert

TAF4 HFD amino acid 827-909 (Taf4 Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pAP2042-1

Plasmid

#105244

Purpose

mammalian eGFP with TEV::3xFlag::Ollas::Myc::V5::HAtag::other linker

Depositor

Geraldine Seydoux

Article

Paix et al Proc Natl Acad Sci U S A. 2017 Nov 28. p

Insert

eGFP with extra-sequence (TEV::3xFlag::Ollas::Myc::V5::HAtag::other linker::eGFP)

Expression

Bacterial

Available Since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pet 3d beta tentacle delete CP

Plasmid

#13300

Depositor

John Cooper

Article

Wear et al Curr Biol. 2003 Sep 2. 13(17):1531-7.

Insert

capping protein alpha 1 beta 1 (deletion 28 amino acids ) (CAPZB Chicken)

Expression

Bacterial

Mutation

CP alpha subunit is intact with a mutated beta C-…

Available Since

Dec. 15, 2006

Availability

Academic Institutions and Nonprofits only
XZ066-(PB)EF1a-LIDARST-CCR6V2-IRESv0-T2A-PuroR-P2A-BFP

Plasmid

#233418

Purpose

PiggyBac donor plasmid with CCR6-V2-ADAR2dd(E488Q,T501A) and ARRB2-tdMCP separated by EMCV IRES

Depositor

Xiaojing Gao

Article

Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

Insert

CCR6-ADARdd-P2A-PuroR-IRES-ARRB2-MCP

Expression

Mammalian

Mutation

E488Q, T501A on ADAR2dd

Promoter

EF1a

Available Since

June 13, 2025

Availability

Academic Institutions and Nonprofits only

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XZ079 (PB)EF1a-CCR6-ADARdd-P2A-PuroR-IRES-ARRB2-MCP

Plasmid

#233435

Purpose

PiggyBac donor plasmid with CCR6-V2-ADAR2dd(E488Q,T501A) and ARRB2-tdMCP separated by EMCV IRES

Depositor

Xiaojing Gao

Article

Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

Insert

CCR6-ADARdd-P2A-PuroR-IRES-ARRB2-MCP

Expression

Mammalian

Mutation

E488Q, T501A on ADAR2dd

Promoter

EF1a

Available Since

April 2, 2025

Availability

Academic Institutions and Nonprofits only

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LSM042 (PB)EF1a-AVPR-ADARdd-P2A-PuroR-IRES-ARRB2-MCP

Plasmid

#233426

Purpose

PiggyBac donor plasmid AVPR2-V2-ADAR2dd(E488Q,T501A) and ARRB2-tdMCP separated by EMCV IRES

Depositor

Xiaojing Gao

Article

Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

Insert

AVPR-ADARdd-P2A-PuroR-IRES-ARRB2-MCP

Expression

Mammalian

Mutation

E488Q, T501A on ADAR2dd

Promoter

EF1a

Available Since

April 2, 2025

Availability

Academic Institutions and Nonprofits only

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XZ388 (IVT) CMV-TO-T7-EGFP-SGc(stem-1(3xMS2))cSG-Cre-hybridUTR3

Plasmid

#233424

Purpose

IVT template for LIDAR reporter mRNA; EGFP as marker, Cre recombinase as output

Depositor

Xiaojing Gao

Article

Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

Insert

T7-EGFP-SGc(stem-1(3xMS2))cSG-Cre-hybridUTR3

Expression

Mammalian

Mutation

WT

Promoter

CMV

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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XZ034 CMV-TO-CCL20

Plasmid

#233416

Purpose

plasmid expressing secreted soluble CCL20

Depositor

Xiaojing Gao

Article

Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

Insert

CCL20 (CCL20 Human)

Expression

Mammalian

Mutation

WT

Promoter

CMV

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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XZ362 EF1a-VEGFR2sp-HA-VEGFR2(Ecto+TM)-TS-tdMCP-export

Plasmid

#233381

Purpose

Optimized VEGF-sensing eLIDAR with VEGFR2 ecto+TM domains fused with tdMCP, with Golgi traffic sequence and ER export sequence

Depositor

Xiaojing Gao

Article

Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

Insert

VEGFR2(ecto+TM)-tdMCP

Tags

HA

Expression

Mammalian

Mutation

WT

Promoter

EF1a

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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XZ363 EF1a-VEGFR1sp-3xFlag-VEGFR1(ecto+TM)-TS-ADAR2dd(QA)-export

Plasmid

#233382

Purpose

Optimized VEGF-sensing eLIDAR with VEGFR1 ecto+TM domains fused with ADAR2dd, with Golgi traffic sequence and ER export sequence

Depositor

Xiaojing Gao

Article

Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

Insert

VEGFR1(ecto+TM)-ADAR2dd

Tags

FLAG

Expression

Mammalian

Mutation

E488Q, T501A on ADAR2dd

Promoter

EF1a

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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XZ375 EF1a-IL2Rbsp-3xFLAG-IL2Rb(ECD+TM)-TS-ADAR2dd-export

Plasmid

#233386

Purpose

Optimized IL-2-sensing eLIDAR with IL-2 beta chain ecto+TM domains fused with ADAR2dd, with Golgi traffic sequence and ER export sequence

Depositor

Xiaojing Gao

Article

Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

Insert

IL2Rb(ecto+TM)-ADAR2dd

Tags

FLAG

Expression

Mammalian

Mutation

E488Q, T501A on ADAR2dd

Promoter

EF1a

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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XZ374 EF1a-IL2Rgsp-3xHA-IL2Rg(ECD+TM)-TS-tdMCP-export

Plasmid

#233389

Purpose

Optimized IL-2-sensing eLIDAR with IL-2 gamma chain ecto+TM domains fused with tdMCP, with Golgi traffic sequence and ER export sequence

Depositor

Xiaojing Gao

Article

Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

Insert

IL2Rg(ecto+TM)-tdMCP

Tags

HA

Expression

Mammalian

Mutation

WT

Promoter

EF1a

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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