We narrowed to 3,091 results for: FRI

1. TRE reporter backbone

   Plasmid

   #158678

   Purpose

   This plasmid serves as the backbone for the TRE reporters described in our paper. It's a promoterless-mStrawberry-pGK-BSD backbone to which we insert a pathway specific promoter before the mStrawberry

   Depositor

   Ravid Straussman

   Article

   Kaufman et al Nat Commun. 2022 May 18;13(1):2725. doi:

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   mStrawberry

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-hSynapsin1-axon-GCaMP6s-P2A-mRuby3

   Plasmid

   #112005

   Purpose

   For enriched expression of GCaMP6s in axons with cytosolic expression of mRuby3

   Depositor

   Lin Tian

   Article

   Broussard et al Nat Neurosci. 2018 Sep;21(9):1272-1280.

   Has Service

   AAV5 and AAV9

   Insert

   axon-GCaMP6s-P2A-mRuby3
   

   Use

   AAV

   Tags

   GAP43 palmitoylation domain

   Expression

   Mammalian

   Mutation

   Promoter

   hSynapsin1

   Available since

   June 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. iRFP-H2A

   Plasmid

   #158691

   Purpose

   This plasmid encodes an iRFP fused to H2A histone nuclear marker. It has CMV promoter driving the expression of iRFP fused to H2A. It has no selection markers

   Depositor

   Ravid Straussman

   Article

   Kaufman et al Nat Commun. 2022 May 18;13(1):2725. doi:

   Insert

   iRFP670
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pPS

   Plasmid

   #85801

   Purpose

   Backbone for pooled cloning of Perturb-seq libraries

   Depositor

   Aviv Regev

   Article

   Dixit et al Cell. 2016 Dec 15;167(7):1853-1866.e17.

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   March 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-hSynapsin1-FLEx-axon-GCaMP6s

   Plasmid

   #112010

   Purpose

   For cre-dependent enriched expression of GCaMP6s in axons

   Depositor

   Lin Tian

   Article

   Broussard et al Nat Neurosci. 2018 Sep;21(9):1272-1280.

   Has Service

   AAV5 and AAV9

   Insert

   axon-GCaMP6s
   

   Use

   AAV and Cre/Lox

   Tags

   GAP43 palmitoylation domain

   Expression

   Mammalian

   Mutation

   Promoter

   hSynapsin1

   Available since

   June 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. XE120 CAMKII-K42M 1-271-CS2+

   Plasmid

   #16734

   Depositor

   Randall Moon

   Article

   Kühl et al J Biol Chem. 2000 Apr 28. 275(17):12701-

   Insert

   CAMKII-K42M 1-271 (Camk2a Rat)
   

   Use

   Xenopus expression

   Tags

   Expression

   Mutation

   C-terminal truncation of K42M kinase

   Promoter

   Available since

   March 14, 2008

   Availability

   Academic Institutions and Nonprofits only

7. Geminin-mStrawberry reporter

   Plasmid

   #158690

   Purpose

   This plasmid is a Geminin cell cycle reporter. It has CMV promoter driving the expression of Geminin fused to mStrawberry-pGK-BSD

   Depositor

   Ravid Straussman

   Article

   Kaufman et al Nat Commun. 2022 May 18;13(1):2725. doi:

   Insert

   mStrawberry
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pENTR221 UBR5 ∆UBR

   Plasmid

   #81063

   Purpose

   Gateway entry vector encoding full length UBR5 W1235L (∆UBR)

   Depositor

   Darren Saunders

   Article

   Shearer et al Mol Biol Cell. 2018 May 9:mbcE17040248.

   Insert

   Ubiquitin protein ligase E3 component N-recognin 5 (UBR5) ∆UBR (UBR5 Human)
   

   Use

   Cloning

   Tags

   Expression

   Mutation

   W1235L (bp g3704t)

   Promoter

   N/A

   Available since

   Jan. 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCAG-Voltron

   Plasmid

   #119033

   Purpose

   Express Voltron in mammalian cells

   Depositor

   Eric Schreiter

   Article

   Abdelfattah et al Science. 2019 Aug 1. pii: science.aav641

   Insert

   Voltron
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Dec. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pENTR221 UBR5 ∆HECT

    Plasmid

    #81065

    Purpose

    Gateway entry vector encoding full length UBR5 C2768A (∆HECT)

    Depositor

    Darren Saunders

    Article

    Shearer et al Mol Biol Cell. 2018 May 9:mbcE17040248.

    Insert

    Ubiquitin protein ligase E3 component N-recognin 5 (UBR5) ∆HECT (UBR5 Human)
    

    Use

    Cloning

    Tags

    Expression

    Mutation

    C2768A (bp t8302g and g8303c)

    Promoter

    N/A

    Available since

    Jan. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pT3TS nErCas12an

    Plasmid

    #132642

    Purpose

    Used for in vitro transcription of dual NLS ErCas12a mRNA

    Depositor

    Stephen Ekker

    Article

    Wierson et al CRISPR J. 2019 Dec;2(6):417-433. doi: 10

    Insert

    ErCas12a
    

    Use

    CRISPR; In vitro transcription

    Tags

    SV40 NLS

    Expression

    Mutation

    Promoter

    T3

    Available since

    Nov. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. Myc-Bnip3FL

    Plasmid

    #100796

    Purpose

    Mammalian expression of Bnip3 full length splice variant

    Depositor

    Joseph Gordon

    Article

    Diehl-Jones et al J Pediatr Gastroenterol Nutr. 2015 Nov;6

    Insert

    mus Bnip3 (Bnip3 Mouse)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. PB-TRE-GNL

    Plasmid

    #204499

    Purpose

    Expresses Glis1, Nanog and lin28a by tet-on promoter in mammalian cells

    Depositor

    Wataru Fujii

    Article

    Matsuya et al Sci Rep. 2024 Feb 8;14(1):3204. doi: 10.

    Insert

    Glis1 (Glis1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET28-AviTag-DogTag-MBP

    Plasmid

    #171773

    Purpose

    Expresses AviTag-DogTag-Maltose binding protein in bacterial cytoplasm. The AviTag enables site-specific biotinylation by BirA.

    Depositor

    Mark Howarth

    Article

    Keeble et al Cell Chem Biol. 2021 Jul 28. pii: S2451-

    Insert

    AviTag-DogTag-MBP
    

    Use

    Tags

    His6 and Thrombin cleavage site

    Expression

    Bacterial

    Mutation

    Peptide tag added to N-terminus of MBP via a GSGE…

    Promoter

    T7

    Available since

    July 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. HA-BirA*-pDEST-N-pcDNA5/FRT/TO

    Plasmid

    #118375

    Purpose

    Expression vector for BioID in mammalian system. Gateway compatible destination vector to generate stable, inducible cell line expressing N-terminally tagged HABirA* and protein of interest.

    Depositor

    Maria Vartiainen

    Article

    Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

    Type

    Empty backbone

    Use

    Destination

    Tags

    HA-BirA*

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. RS35075_pMQ30_KO_Kan

    Plasmid

    #185390

    Purpose

    Non-replicating vector used to create markerless deletion of RR42_RS35075 in C. basilensis 4G11

    Depositor

    Adam Arkin

    Article

    Sander et al Biotechnol Bioeng. 2024 Jan;121(1):139-1

    Inserts

    RR42_RS35075 upstream flanking homology region
    RR42_RS35075 downstream flanking homology region
    nptII kanamycin resistance marker
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. h-Plekhm1_EGFP

    Plasmid

    #73836

    Purpose

    Express human Plekhm1 in mammalian cells

    Depositor

    Paul Odgren

    Article

    Van Wesenbeeck et al J Clin Invest. 2007 Apr;117(4):919-30.

    Insert

    Plekhm1 (PLEKHM1 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pICE-NLS-mCherry

    Plasmid

    #60364

    Purpose

    Plasmid for expression of NLS-mCherry (control plasmid). Confers resistance to Puromycin. Expression is inducible in T-REx cells.

    Depositor

    Sébastien Britton, Patrick Calsou

    Article

    Britton et al Nucleic Acids Res. 2014 Oct 1;42(14):904

    Insert

    NLS-mCherry
    

    Use

    Tags

    NLS from SV40 T antigen and mCherry

    Expression

    Mutation

    Promoter

    CMV-tet

    Available since

    Jan. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1-EPG

    Plasmid

    #117978

    Purpose

    Express EPG in mamalian cells

    Depositor

    Assaf Gilad

    Article

    Krishnan et al Sci Rep. 2018 Jun 8;8(1):8764. doi: 10.1

    Insert

    Electromagnetic perceptive gene
    

    Use

    Tags

    HIS and v5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCMX IkB alpha M

    Plasmid

    #12329

    Depositor

    Inder Verma

    Article

    Van Antwerp et al Science. 1996 Nov 1. 274(5288):787-9.

    Insert

    IkB alpha M (Nfkbia Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Dominant-negative mutant. Many signal transductio…

    Promoter

    Available since

    Aug. 17, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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