We narrowed to 11,224 results for: NSI;

1. RS18965_pMQ30_KO_Kan

   Plasmid

   #185394

   Purpose

   Non-replicating vector used to create markerless deletion of RR42_RS18965 in C. basilensis 4G11

   Depositor

   Adam Arkin

   Article

   Sander et al Biotechnol Bioeng. 2024 Jan;121(1):139-1

   Inserts

   RR42_RS18965 upstream flanking homology region
   RR42_RS18965 downstream flanking homology region
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. RS18970_pMQ30_KO_Kan

   Plasmid

   #185395

   Purpose

   Non-replicating vector used to create markerless deletion of RR42_RS18970 in C. basilensis 4G11

   Depositor

   Adam Arkin

   Article

   Sander et al Biotechnol Bioeng. 2024 Jan;121(1):139-1

   Inserts

   RR42_RS18970 upstream flanking homology region
   RR42_RS18970 downstream flanking homology region
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCDNA4.TO_ORF18_TurboID_3xHA

   Plasmid

   #200017

   Purpose

   Expresses KSHV protein ORF18 fused at the C-terminal to TurboID-3xHA

   Depositor

   Britt Glaunsinger

   Article

   McCollum et al PLoS Pathog. 2023 Apr 17;19(4):e1011163.

   Insert

   ORF18
   

   Use

   Tags

   TurboID-3xHA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCDNA4.TO_ORF30_TurboID_3xHA

   Plasmid

   #200018

   Purpose

   Expresses KSHV protein ORF30 fused at the C-terminal to TurboID-3xHA

   Depositor

   Britt Glaunsinger

   Article

   McCollum et al PLoS Pathog. 2023 Apr 17;19(4):e1011163.

   Insert

   ORF30
   

   Use

   Tags

   TurboID-3xHA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCDNA4.TO_ORF31_TurboID_3xHA

   Plasmid

   #200019

   Purpose

   Expresses KSHV protein ORF31 fused at the C-terminal to TurboID-3xHA

   Depositor

   Britt Glaunsinger

   Article

   McCollum et al PLoS Pathog. 2023 Apr 17;19(4):e1011163.

   Insert

   ORF31
   

   Use

   Tags

   TurboID-3xHA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCDNA4.TO_ORF66_TurboID_3xHA

   Plasmid

   #200020

   Purpose

   Expresses KSHV protein ORF66 fused at the C-terminal to TurboID-3xHA

   Depositor

   Britt Glaunsinger

   Article

   McCollum et al PLoS Pathog. 2023 Apr 17;19(4):e1011163.

   Insert

   ORF66
   

   Use

   Tags

   TurboID-3xHA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCDNA4.TO_3xHA_TurboID_ORF34

   Plasmid

   #200021

   Purpose

   Expresses KSHV protein ORF34 fused at the N-terminal to 3xHA-TurboID

   Depositor

   Britt Glaunsinger

   Article

   McCollum et al PLoS Pathog. 2023 Apr 17;19(4):e1011163.

   Insert

   ORF34
   

   Use

   Tags

   3xHA-TurboID

   Expression

   Mammalian

   Mutation

   Promoter

   CMV promoter

   Available since

   July 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLJM1-zeo G226A ORF29

   Plasmid

   #200024

   Purpose

   Lentiviral vector for expression of KSHV ORF29 with G226A mutation

   Depositor

   Britt Glaunsinger

   Article

   McCollum et al PLoS Pathog. 2023 Apr 17;19(4):e1011163.

   Insert

   ORF29
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Glycine 226 changed to alanine

   Promoter

   CMV

   Available since

   July 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pUBQ10:WNFLS2-2

   Plasmid

   #202190

   Purpose

   Express 'Washington navel' Orange FLS2-2 gene under the UBQ10 promoter

   Depositor

   Gitta Coaker

   Article

   Trinh et al Plant Physiol. 2023 May 5:kiad263. doi:

   Insert

   Flagellin sensing 2
   

   Use

   Tags

   HA

   Expression

   Plant

   Mutation

   Promoter

   AtUBQ10

   Available since

   July 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pUBQ10:WNFLS2-1

    Plasmid

    #202189

    Purpose

    Express 'Washington navel' Orange FLS2-1 gene under the UBQ10 promoter

    Depositor

    Gitta Coaker

    Article

    Trinh et al Plant Physiol. 2023 May 5:kiad263. doi:

    Insert

    Flagellin sensing 2
    

    Use

    Tags

    HA

    Expression

    Plant

    Mutation

    Promoter

    AtUBQ10

    Available since

    July 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pUE1-TSP-ORF68 R14A/K310A

    Plasmid

    #162655

    Purpose

    Expresses ORF68 R14A/K310A in mammalian cells with an N-terminal TwinStrep tag and HRV 3C protease cleavage site

    Depositor

    Britt Glaunsinger

    Article

    Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

    Insert

    ORF68
    

    Use

    Tags

    N-terminal 2xStrep-Tag II and HRV 3C site

    Expression

    Mammalian

    Mutation

    Amino acids R14 and K310 mutated to alanine

    Promoter

    CMV

    Available since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLJM1-zeo-2xStrep-ORF68 C52A

    Plasmid

    #162660

    Purpose

    Lentiviral vector for stable expression of N-terminal 2xStrep-tagged ORF68 C52A in mammalian cells

    Depositor

    Britt Glaunsinger

    Article

    Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

    Insert

    ORF68
    

    Use

    Lentiviral

    Tags

    N-terminal 2xStrep-Tag II

    Expression

    Mammalian

    Mutation

    Amino acid C52 mutated to alanine

    Promoter

    CMV

    Available since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA4/TO-2xStrep-ORF68 K174A/R179A/K182A

    Plasmid

    #162639

    Purpose

    Expresses N-terminal 2x-Strep-tagged ORF68 K174A/R179A/K182A in mammalian cells

    Depositor

    Britt Glaunsinger

    Article

    Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

    Insert

    ORF68
    

    Use

    Tags

    N-terminal 2xStrep-Tag II

    Expression

    Mammalian

    Mutation

    Amino acids K174, R179, and K182 mutated to alani…

    Promoter

    CMV

    Available since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pUE1-TSP-ORF68 K435A

    Plasmid

    #162651

    Purpose

    Expresses ORF68 K435A in mammalian cells with an N-terminal TwinStrep tag and HRV 3C protease cleavage site

    Depositor

    Britt Glaunsinger

    Article

    Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

    Insert

    ORF68
    

    Use

    Tags

    N-terminal 2xStrep-Tag II and HRV 3C site

    Expression

    Mammalian

    Mutation

    Amino acid K435 mutated to alanine

    Promoter

    CMV

    Available since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pUE1-TSP-ORF68 K450A/R451A

    Plasmid

    #162653

    Purpose

    Expresses ORF68 K450A/R451A in mammalian cells with an N-terminal TwinStrep tag and HRV 3C protease cleavage site

    Depositor

    Britt Glaunsinger

    Article

    Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

    Insert

    ORF68
    

    Use

    Tags

    N-terminal 2xStrep-Tag II and HRV 3C site

    Expression

    Mammalian

    Mutation

    Amino acids K450 and R451 mutated to alanine

    Promoter

    CMV

    Available since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. AAV-Synapsin-mScarlet-optoJNKi-WPRE

    Plasmid

    #159968

    Purpose

    optogenetic JNK inhibitor with increased RET from LOV2 to reduce actuation sensitivity, detectable without activation

    Depositor

    Michael Courtney

    Article

    Li et al Nat Commun. 2020 Oct 9;11(1):5107. doi:

    Insert

    mScarlet-optoJNKi
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    synthetic

    Promoter

    hSyn1

    Available since

    Oct. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. AAV-Synapsin-mTurquoise2-optoJNKi-WPRE

    Plasmid

    #159966

    Purpose

    optogenetic JNK inhibitor with increased RET to LOV2 to increase actuation sensitivity

    Depositor

    Michael Courtney

    Article

    Li et al Nat Commun. 2020 Oct 9;11(1):5107. doi:

    Insert

    mTurquoise2-optoJNKi
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    synthetic

    Promoter

    hSyn1

    Available since

    Oct. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. AAV-Synapsin-Ypet-optoJNKi-WPRE

    Plasmid

    #159967

    Purpose

    optogenetic JNK inhibitor with increased RET from LOV2 to reduce actuation sensitivity, detectable without activation

    Depositor

    Michael Courtney

    Article

    Li et al Nat Commun. 2020 Oct 9;11(1):5107. doi:

    Insert

    Ypet-optoJNKi
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    synthetic

    Promoter

    hSyn1

    Available since

    Oct. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA4/TO-mu24 1-181-2xStrep

    Plasmid

    #138444

    Purpose

    Expresses truncated mu24 with a C-terminal 2xStrep tag in mammalian cells

    Depositor

    Britt Glaunsinger

    Article

    Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

    Insert

    mu24
    

    Use

    Tags

    Strep

    Expression

    Mammalian

    Mutation

    Amino acids 1-181

    Promoter

    CMV

    Available since

    Oct. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA4/TO-mu24 1-191-2xStrep

    Plasmid

    #138445

    Purpose

    Expresses truncated mu24 with a C-terminal 2xStrep tag in mammalian cells

    Depositor

    Britt Glaunsinger

    Article

    Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

    Insert

    mu24
    

    Use

    Tags

    Strep

    Expression

    Mammalian

    Mutation

    Amino acids 1-191

    Promoter

    CMV

    Available since

    Oct. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge