We narrowed to 42,086 results for: STI;

1. pAAV-CAG-FLEX-rc [Chronos-tdTomato]

   Plasmid

   #84484

   Purpose

   AAV-mediated expression of Chronos-tdTomato under the CAG promoter, in floxed/reversed (Cre-dependent) manner. Using bGHpA signal. tdTomato is a codon diversified version.

   Depositor

   Edward Boyden

   Article

   Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

   Insert

   Chronos-tdTomato
   

   Use

   AAV

   Tags

   tdTomato

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   April 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCMV-T7-ABEmax(7.10)-SpRY-P2A-EGFP (RTW5025)

   Plasmid

   #140003

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized ABEmax(7.10) A-to-G base editor with SpRY(D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R) and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Walton et al Science. 2020 Apr 17;368(6488):290-296.

   Insert

   human codon optimized ABEmax(7.10) SpCas9 variant named SpRY with P2A-EGFP
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   P2A-EGFP

   Expression

   Mammalian

   Mutation

   nSpCas9=D10A; SpRY=A61R/L1111R/D1135L/S1136W/G121…

   Promoter

   CMV and T7

   Available since

   March 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pET21b(+)-Is-PETase-W159H-S238F

   Plasmid

   #112203

   Purpose

   pET-21b(+) based plasmid for expression of PETase from Ideonella sakaiensis 201-F6 (Genbank GAP38373.1) with W159H and S238F mutations, codon optimized for expression in E. coli K12

   Depositor

   Christopher Johnson, Gregg Beckham

   Article

   Austin et al Proc Natl Acad Sci U S A. 2018 May 8;115

   Insert

   PETase gene from Ideonella sakaiensis 201-F6 with W159H and S238F mutations, codon optimized for expression in E. coli K12
   

   Use

   Tags

   6XHIS

   Expression

   Bacterial

   Mutation

   W159H, S238F

   Promoter

   N/A

   Available since

   July 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pMS73

   Plasmid

   #110629

   Purpose

   CRISPR/Cas9 vector for mutliplexed genome editing in Ustilago maydis. Expresses U. maydis codon-optimized Cas9 under the hsp70 promoter, contains a short version of U6 promoter, is self-replicating

   Depositor

   Regine Kahmann

   Article

   Schuster et al Fungal Genet Biol. 2018 Mar;112:21-30. d

   Inserts

   short U6 promoter
   cas9
   tnos terminator
   

   Use

   CRISPR; Self-replicating in ustilago maydis, conf…

   Tags

   N-terminal NLS, C-terminal HA-tag +NLS

   Expression

   Bacterial

   Mutation

   Streptococcus pyogenes gene codon-optimized for U…

   Promoter

   U. maydis hsp70 promoter (Kronstad and Leong, 198…

   Available since

   May 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pAAV-Syn-Chronos-tdTomato

   Plasmid

   #62726

   Purpose

   AAV-mediated expression of Chronos-tdTomato under the Syn promoter. Using bGHpA signal. tdTomato has codons varied between the first and second tandem repeats to reduce recombination.

   Depositor

   Edward Boyden

   Article

   Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

   Has Service

   AAV8

   Insert

   Chronos-tdTomato
   

   Use

   AAV

   Tags

   tdTomato

   Expression

   Mammalian

   Mutation

   Promoter

   human synapsin promoter

   Available since

   April 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pAAV-Syn-FLEX-rc[Chronos-GFP]

   Plasmid

   #62722

   Purpose

   AAV-mediated expression of Chronos-GFP under the Syn promoter, in floxed/reversed (Cre-dependent) manner. Using bGHpA signal.

   Depositor

   Edward Boyden

   Article

   Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

   Insert

   Chronos-GFP
   

   Use

   AAV

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   human synapsin promoter

   Available since

   April 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pET His6 MBP TEV HDAC8_374

   Plasmid

   #122174

   Purpose

   Expresses a truncated construct of histone deacetylase 8 for crystallography bearing an N-terminal tobacco etch virus protease-cleavable hexahistidine-maltose binding protein tag

   Depositor

   David Christianson

   Article

   Porter et al ACS Chem Biol. 2017 Sep 15;12(9):2281-22

   Insert

   His6 MBP TEV HDAC8_374 (HDAC8 Human)
   

   Use

   Tags

   hexahistidine tag and tobacco etch virus protease…

   Expression

   Bacterial

   Mutation

   Residues Met1–Pro7 and H375-V377 have been delete…

   Promoter

   T7

   Available since

   March 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAAV-Syn-FLEX-rc [Chronos-tdTomato]

   Plasmid

   #84481

   Purpose

   AAV-mediated expression of Chronos-tdTomato under the Syn promoter, in floxed/reversed (Cre-dependent) manner. Using bGHpA signal. tdTomato is a codon diversified version.

   Depositor

   Edward Boyden

   Article

   Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

   Insert

   Chronos-tdTomato
   

   Use

   AAV

   Tags

   tdTomato

   Expression

   Mammalian

   Mutation

   Promoter

   human synapsin promoter

   Available since

   April 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. FUW-Flag-Mbd3 delta1-70

   Plasmid

   #52372

   Purpose

   Lentiviral expression vector for constitutive expression of double Flag tagged Mbd3 mutant

   Depositor

   Jacob Hanna

   Article

   Rais et al Nature. 2013 Oct 3;502(7469):65-70. doi:

   Insert

   Mbd3 (Mbd3 Mouse)
   

   Use

   Lentiviral

   Tags

   flag

   Expression

   Mammalian

   Mutation

   deleted amino acids 1-70

   Promoter

   Ubiquitin

   Available since

   Sept. 8, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. FUW-Flag-Mbd3 delta 25-83

    Plasmid

    #52373

    Purpose

    Lentiviral expression vector for constitutive expression of double Flag tagged Mbd3 mutant

    Depositor

    Jacob Hanna

    Article

    Rais et al Nature. 2013 Oct 3;502(7469):65-70. doi:

    Insert

    Mbd3 (Mbd3 Mouse)
    

    Use

    Lentiviral

    Tags

    flag

    Expression

    Mammalian

    Mutation

    amino acids 25-83 deleted

    Promoter

    Ubiquitin

    Available since

    Sept. 8, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. FUW-Flag-Mbd3 delta49-63

    Plasmid

    #52374

    Purpose

    Lentiviral expression vector for constitutive expression of double Flag tagged Mbd3 mutant

    Depositor

    Jacob Hanna

    Article

    Rais et al Nature. 2013 Oct 3;502(7469):65-70. doi:

    Insert

    Mbd3 (Mbd3 Mouse)
    

    Use

    Lentiviral

    Tags

    flag

    Expression

    Mammalian

    Mutation

    deleted amino acids 49-63

    Promoter

    Ubiquitin

    Available since

    Sept. 8, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAAV-Syn-FLEX-fd [Chronos-GFP]

    Plasmid

    #84482

    Purpose

    AAV-mediated expression of Chronos-GFP under the Syn promoter, in floxed/forward (Cre-dependent) manner. Cre turns gene off. Using bGHpA signal.

    Depositor

    Edward Boyden

    Article

    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

    Insert

    Chronos-GFP
    

    Use

    AAV

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    human synapsin promoter

    Available since

    July 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAAV-EF1α1.1-Chronos-tdTomato

    Plasmid

    #122099

    Purpose

    AAV-mediated expression of Chronos-tdTomato under the EF1α promoter (1.1kb short version). tdTomato has codons varied to reduce recombination. Using SV40 pA signal.

    Depositor

    Edward Boyden

    Article

    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

    Insert

    Chronos-tdTomato
    

    Use

    AAV

    Tags

    tdTomato

    Expression

    Mammalian

    Mutation

    Promoter

    EF1α (1.1 kb short version)

    Available since

    Aug. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. AAV-EF1α1.1-FLPX-rc [Chronos-tdTomato]

    Plasmid

    #122103

    Purpose

    AAV-mediated expression of Chronos-tdTomato under the EF1α1.1 promoter in frt/reversed (Flp-dependent) manner. tdTomato has codons varied to reduce recombination.

    Depositor

    Edward Boyden

    Article

    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

    Insert

    Chronos-tdTomato
    

    Use

    AAV

    Tags

    tdTomato

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a（1.1kb short version)

    Available since

    Sept. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAAV-EF1α1.1-FLEX-rc [Chronos-GFP]

    Plasmid

    #84485

    Purpose

    AAV-mediated expression of Chronos-GFP under the EF1α promoter (1.1kb short version), in floxed/reversed (Cre-dependent) manner. Using bGHpA signal.

    Depositor

    Edward Boyden

    Article

    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

    Insert

    Chronos-GFP
    

    Use

    AAV

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1α, 1.1 kb long (short version)

    Available since

    April 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pBRPy CAGGS-Mbd3-IRES-PURO

    Plasmid

    #52376

    Purpose

    Mammalian expression vector for constitutive expression of Mbd3

    Depositor

    Jacob Hanna

    Article

    Rais et al Nature. 2013 Oct 3;502(7469):65-70. doi:

    Insert

    Mbd3 (Mbd3 Mouse)
    

    Use

    Tags

    2xFlag (DYKDDK)

    Expression

    Mammalian

    Mutation

    Promoter

    CAGGS

    Available since

    Sept. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. V37m

    Plasmid

    #121043

    Purpose

    MoClo golden gate assembly AE destination vector DVCK-AE-mScar (ColE1/kan resistance destination vector with constitutive mScarlet expressing for screening.

    Depositor

    Richard Murray

    Insert

    Kan destination vector with ColE1 origin and mScarlet selection
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pMalAnPL

    Plasmid

    #67281

    Purpose

    Expresses MBP-Anacystis nidulans photolyase in E. coli

    Depositor

    Aziz Sancar

    Insert

    photolyase Anacystis nidulans
    

    Use

    Tags

    maltose binding protein

    Expression

    Bacterial

    Mutation

    none known

    Promoter

    Available since

    July 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCMK

    Plasmid

    #62818

    Purpose

    Bacterial constitutive S. pyogenes Cas9 expression plasmid

    Depositor

    Sébastien Rodrigue

    Insert

    Cas9
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. V35m

    Plasmid

    #121042

    Purpose

    MoClo golden gate assembly AE destination vector DVCK-AE-GFP (ColE1/kan resistance destination vector with constitutive GFP expression for screening.

    Depositor

    Richard Murray

    Insert

    Kan destination vector with ColE1 origin and GFP selection
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge