We narrowed to 5,295 results for: pcdna3.1

1. pcDNA3.1-CibN-dCas9-CibN

   Plasmid

   #60553

   Purpose

   Expresses CibN-dCas9-CibN in mammalian cells

   Depositor

   Charles Gersbach

   Article

   Polstein et al Nat Chem Biol. 2015 Feb 9. doi: 10.1038/

   Insert

   dCas9-CibN
   

   Use

   CRISPR

   Tags

   FLAG and SV40 NLS

   Expression

   Mammalian

   Mutation

   inactivating Cas9 mutations D10A and H840A

   Promoter

   CMV

   Available since

   Feb. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1/Myc-His beta4

   Plasmid

   #16039

   Depositor

   Filippo Giancotti

   Article

   Dans et al J Biol Chem. 2001 Jan 12. 276(2):1494-50

   Insert

   beta4 (ITGB4 Human)
   

   Use

   Tags

   His and Myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 2, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1-GFP-FUS-IDR

   Plasmid

   #183236

   Purpose

   Expression of eGFP fused to a intrinsically disordered regions of the FUS protein

   Depositor

   Jorge Ferrer

   Article

   Beucher et al Nat Cell Biol. 2022 Oct 6. pii: 10.1038/

   Insert

   EGFP
   

   Use

   Tags

   FLAG tag and Fusion to an IDR from the FUS protei…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1/Myc-His(-)-HSulf-1

   Plasmid

   #13003

   Depositor

   Steven Rosen

   Article

   Morimoto-Tomita et al J Biol Chem. 2002 Dec 20. 277(51):49175-

   Insert

   human sulfatase 1 (SULF1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 30, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1-cyto-KB1691-Nluc

   Plasmid

   #204326

   Purpose

   GPCR/G protein BRET biosensor

   Depositor

   Mikel Garcia-Marcos

   Article

   Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

   Insert

   cytosolic KB1691-Nluc
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 19, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pcDNA3.1/opto-a1AR-EYFP

   Plasmid

   #20947

   Purpose

   Mammalian expression of opto-a1AR fused to EYFP for optical control of GPCR signaling

   Depositor

   Karl Deisseroth

   Article

   Airan et al Nature. 2009 Apr 23;458(7241):1025-9. do

   Insert

   opto-a1AR
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 28, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1-SARS-CoV-2-S (Wuhan)

   Plasmid

   #215812

   Purpose

   Expresses SARS-CoV-2 Spike protein in mammalian cells

   Depositor

   Thomas Gallagher

   Article

   Qing et al mBio. 2021 Aug 31;12(4):e0159021. doi: 1

   Insert

   SARS-CoV-2 Spike (S )
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. mScarletI-T2A-mTq2 pcDNA3.1

   Plasmid

   #230048

   Purpose

   Expresses mScarletI-T2A-mTq2 in human cells

   Depositor

   Bas Teusink

   Article

   Botman et al bioRxiv 2024.11.29.626064

   Insert

   mScarletI-T2A-mTq2
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 23, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pcDNA3.1+_FH-AGO2-5xA

   Plasmid

   #92007

   Purpose

   Expresses FLAG-HA-AGO2 [S824A, S828A, T830A, S831A, S834A (5xA)]

   Depositor

   Joshua Mendell

   Article

   Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

   Insert

   AGO2 (AGO2 Human)
   

   Use

   Tags

   FLAG-HA

   Expression

   Mammalian

   Mutation

   S824A, S828A, T830A, S831A, S834A (5xA)

   Promoter

   Available since

   Aug. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1-CD63-(TEV)-tTA

    Plasmid

    #162597

    Purpose

    human CD63-TEV cleavage site-tTA

    Depositor

    Masaharu Somiya

    Article

    Somiya et al J Extracell Vesicles. 2021 Nov;10(13):e1

    Insert

    human CD63 (CD63 Human)
    

    Use

    Tags

    TEV protease cleavage site and tTA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter

    Available since

    Feb. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.1-Rho-EGFP-ETSQVAPA

    Plasmid

    #45399

    Depositor

    David Williams

    Article

    Trivedi et al J Neurosci. 2012 Aug 1;32(31):10587-93.

    Insert

    Rhodopsin (RHO Bovine)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 16, 2013

    Availability

    Academic Institutions and Nonprofits only

12. pcDNA3.1+_FH-AGO2-5xE

    Plasmid

    #92008

    Purpose

    Expresses FLAG-HA-AGO2 [S824E, S828E, T830E, S831E, S834E (5xE)]

    Depositor

    Joshua Mendell

    Article

    Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

    Insert

    AGO2 (AGO2 Human)
    

    Use

    Tags

    FLAG-HA

    Expression

    Mammalian

    Mutation

    S824E, S828E, T830E, S831E, S834E (5xE)

    Promoter

    Available since

    Aug. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.1/NDUFS3/myc-His

    Plasmid

    #127487

    Purpose

    Expresses myc-tagged NDUFS3 in mammalian cells

    Depositor

    Dieter Wolf, Shashi Jain

    Article

    Jain et al Cell Chem Biol. 2021 Nov 23. pii: S2451-

    Insert

    NDUFS3 (NDUFS3 Human)
    

    Use

    Tags

    myc-His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1_miniSOG2 T2A H2B-EGFP

    Plasmid

    #87410

    Purpose

    Mammalian expression vector encoding Histone 2B GFP fusion and miniSOG2

    Depositor

    Xiaokun Shu

    Article

    Makhijani et al Cell Chem Biol. 2017 Jan 19;24(1):110-11

    Insert

    mini singlet oxygen generator 2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3.1-SARS-CoV-2-HiBiT-N

    Plasmid

    #215815

    Purpose

    Expresses N protein with HiBiT tag in mammalian cells

    Depositor

    Thomas Gallagher

    Article

    Qing et al mBio. 2021 Aug 31;12(4):e0159021. doi: 1

    Insert

    SARS-CoV-2 HiBiT Nucleocapsid (N )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.1-Tat101(AD8)-Flag

    Plasmid

    #115777

    Purpose

    pCMV-Tat101AD8-Flag allows the expression of Tat101(AD8) protein with a C-terminal flag-tag.

    Depositor

    Damian Purcell

    Article

    Khoury et al J Vis Exp. 2019 Jan 22;(143). doi: 10.37

    Insert

    HIV-1 Tat protein (AD8)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    None

    Promoter

    CMV

    Available since

    Oct. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.1+ eiF4A2 myc HIS

    Plasmid

    #71658

    Purpose

    mammalian expression of eiF4A2

    Depositor

    Gideon Dreyfuss

    Insert

    EIF4A2 (EIF4A2 Human)
    

    Use

    Tags

    HIS and myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

18. pcDNA3.1-Lari-BFP2-TSERex

    Plasmid

    #123311

    Purpose

    Expresses the proton pump rhodopsin in mammalian cells under CAG promoter

    Depositor

    Yulong Li

    Article

    Wu et al Elife. 2019 Jan 14;8. pii: 43366. doi: 1

    Insert

    Lari-BFP2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    April 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1(+)/Puro/FA/GFP-N--Grb2-2

    Plasmid

    #112837

    Purpose

    transient/stable overexpression

    Depositor

    Tomasz Prószyński

    Article

    Gingras et al J Cell Sci. 2016 Mar 1;129(5):898-911. d

    Insert

    Grb2 (Grb2 Mouse)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    SH2 + SH3 (C-term) domains of Grb2

    Promoter

    Available since

    Aug. 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3.1D V5-His-TOPO-HuR.wt

    Plasmid

    #110417

    Purpose

    Mammalian expression of HuR (wild type)

    Depositor

    Sandra Martha Gomes Dias

    Insert

    HuR (ELAVL1) (ELAVL1 Human)
    

    Use

    Tags

    6xHis and V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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