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Plasmid#228240PurposeBxb1 attB_wildtype site and SpCas9-hygro for integration into Bxb1 attP_wildtype landing pad (eg. pHPHS232). Includes pEF1 and attP_GA for second round of recombinationDepositorInsertCas9
UseTagsHygromycin phosphotransferaseExpressionMammalianMutationPromoternoneAvailable sinceDec. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28-H12TEV-hEIF5B-587~1220
Plasmid#208637Purposefor E. coli expression truncated human eIF5B (ENST00000289371), aa587~1220DepositorInserthuman eIF5B (ENST00000289371) aa 587~1220 (EIF5B Human)
UseTags6xhisExpressionBacterialMutationcontain SNP rs1376443, protein sequence not chang…PromoterT7Available sinceMay 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pB-Neo-mCherry-mDYNLL1
Plasmid#210336PurposeOverexpression of mDYNLL1DepositorInsertmDYNLL1 (Dynll1 Mouse)
UseTagsmCherryExpressionMammalianMutationPromoterAvailable sinceFeb. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
RGG-BcLOV-mCh-SOS
Plasmid#208278PurposeExpresses RGG-BcLOV-SOS for optogenetic activation of Ras/Erk signaling. Includes an mCherry tag.DepositorInsertRGG-BcLOV-mCh-SOS
UseLentiviralTagsExpressionMutationPromoterpCMVAvailable sinceJan. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
Fus-BcLOV-mCh-EGFR
Plasmid#208281PurposeExpresses Fus-BcLOV-EGFR for optogenetic activation of EGFR signaling. Includes an mCherry tag.DepositorInsertFus-BcLOV-mCh-EGFR
UseLentiviralTagsExpressionMutationPromoterpCMVAvailable sinceJan. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28-RPL3-191~307-mEGFP(A206K)-TwStrep
Plasmid#208626Purposefor E. coli expression of truncated human RPL3 (ENST00000216146), aa191~307, fused with mEGFP(A206K) and Twin Strep tagDepositorInserthuman RPL3 (ENST00000216146) aa 191~307 (RPL3 Human)
UseTags6xHis, Twin Strep, and mEGFP(A206K)ExpressionBacterialMutationonly aa 191~307, other residues not includedPromoterT7Available sinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28-RPL3-299~398-mEGFP(A206K)-TwStrep
Plasmid#208627Purposefor E. coli expression of truncated human RPL3 (ENST00000216146), aa299~398, fused with mEGFP(A206K) and Twin Strep tagDepositorInserthuman RPL3 (ENST00000216146) aa 299~398 (RPL3 Human)
UseTags6xHis, Twin Strep, and mEGFP(A206K)ExpressionBacterialMutationonly aa 299~398, other residues not includedPromoterT7Available sinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28-RPL3-212~285-mEGFP(A206K)-TwStrep
Plasmid#208629Purposefor E. coli expression of truncated human RPL3 (ENST00000216146), aa212~285, fused with mEGFP(A206K) and Twin Strep tagDepositorInserthuman RPL3 (ENST00000216146) aa 212~285 (RPL3 Human)
UseTags6xHis, Twin Strep, and mEGFP(A206K)ExpressionBacterialMutationonly aa 212~285, other residues not includedPromoterT7Available sinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
CRISPR_HLA_DPB
Plasmid#164989PurposeExpression of gRNA targeting HLA-DPB locus, including DPB1*01:01:01DepositorInsertgRNA against HLA-DPB1*01:01:01
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
CRISPR_HLA_DQB
Plasmid#164991PurposeExpression of gRNA targeting HLA-DQB locus, including DQB1*05:01:01DepositorInsertgRNA against HLA-DQB1*05:01:01
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 29, 2022AvailabilityAcademic Institutions and Nonprofits only