We narrowed to 11,108 results for: Kars

1. pTpPuc3

   Plasmid

   #62864

   Purpose

   Episome vector for Thalassiosira pseudonana (pUC-based)

   Depositor

   Hamilton Smith

   Article

   Karas et al Nat Commun. 2015 Apr 21;6:6925. doi: 10.

   Type

   Empty backbone

   Use

   Synthetic Biology; Episomal vector for thalassios…

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 7, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLC133

   Plasmid

   #198639

   Purpose

   Expression plasmid for dCas9 in E. coli used in Chip-seq experiments. dCas9 is tagged with a C-terminal 3x FLAG tag. A guide RNA can be cloned using BsaI restriction sites.

   Depositor

   David Bikard

   Article

   Rostain et al Nucleic Acids Res. 2023 Mar 17:gkad170.

   Insert

   dCas9
   

   Use

   Tags

   3x-FLAG

   Expression

   Bacterial

   Mutation

   Promoter

   pTet

   Available since

   April 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. 6xHis(Thrombin)-GFP(TEV)-Drp1-StrepII

   Plasmid

   #174424

   Purpose

   Expresses human Drp1 Isoform 3 with EGFP at N-terminus in bacteria

   Depositor

   Thomas Pucadyil

   Article

   Kamerkar et al Nat Commun. 2018 Dec 7;9(1):5239. doi: 1

   Insert

   Drp1
   

   Use

   Tags

   6xHis(Thrombin)EGFP(TEV) and StrepII

   Expression

   Bacterial

   Mutation

   None

   Promoter

   T7

   Available since

   Oct. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFD115

   Plasmid

   #124768

   Purpose

   pFD115 carries cas9 controlled by an aTc-inducible Ptet promoter, a sgRNA controlled constitutively from PpflB S. aureus promoter to clone a guide between two BsaI sites and oriT on pLZ12 vector

   Depositor

   David Bikard

   Article

   Depardieu et al Methods. 2019 Aug 1. pii: S1046-2023(18)

   Inserts

   Ptet
   cas9
   term PpflB sgRNA (BsaI)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Ptet

   Available since

   Aug. 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLV-EF1a-MinE(L4E)-EGFP_IRES-Blast

   Plasmid

   #213117

   Purpose

   Expression of a faster-running MinE mutant in mammalian cells

   Depositor

   Scott Coyle

   Article

   Rajasekaran et al Cell. 2023 Dec 27:S0092-8674(23)01339-9.

   Insert

   MinE(L4E)-EGFP (minE Escherichia coli)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   L4E

   Promoter

   EF1a

   Available since

   Jan. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLV-EF1a-FRB-mCherry-MinD-IRES-Puro

   Plasmid

   #213116

   Purpose

   Expression of FRB-mCherry-MinD in mammalian cells for payload delivery

   Depositor

   Scott Coyle

   Article

   Rajasekaran et al Cell. 2023 Dec 27:S0092-8674(23)01339-9.

   Insert

   FRB-mCherry-MinD (minD Escherichia coli)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Jan. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. AcpP pet29a C-terminal His Tag

   Plasmid

   #75016

   Purpose

   Expresses the acyl carrier protein (ACP) from the E. coli fatty acid synthase

   Depositor

   Michael Burkart

   Article

   Worthington et al ACS Chem Biol. 2006 Dec 20;1(11):687-91.

   Insert

   AcpP (acpP E. coli)
   

   Use

   Tags

   His Tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 18, 2016

   Availability

   Academic Institutions and Nonprofits only

8. CRT-GFP pcDNA3.1

   Plasmid

   #80978

   Purpose

   mammalian expression of CRT-GFP

   Depositor

   Peter Steyger

   Article

   Karasawa et al Toxicol Sci. 2011 Dec;124(2):378-87. doi

   Insert

   CRT (Calr Mouse)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   no KDEL

   Promoter

   CMV

   Available since

   Aug. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

9. PB-Antares2

   Plasmid

   #120868

   Purpose

   Piggybac transposon plasmid for live animal optical imaging

   Depositor

   Jordan Green

   Article

   Karlsson et al Adv Funct Mater. 2021 Apr 22;31(17). doi

   Insert

   pcDNA3-Antares2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Antares2 amplicon

   Promoter

   CAG Promoter

   Available since

   Feb. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBabe neo TGFBR3-HA

    Plasmid

    #83095

    Purpose

    Retroviral vector for constitutive TGFBR3 expression with C-terminal HA tag

    Depositor

    Kevin Janes

    Article

    Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

    Insert

    TGFBR3 (TGFBR3 Human)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

11. pLV-EF1a-PKAsub-mCherry-MinD-IRES-Puro

    Plasmid

    #213118

    Purpose

    Expression of one half of a PKAsub-mCherry-MinD reporter circuit in mammalian cells

    Depositor

    Scott Coyle

    Article

    Rajasekaran et al Cell. 2023 Dec 27:S0092-8674(23)01339-9.

    Insert

    PKAsub-mCherry-MinD (minD Escherichia coli)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEGFP-RapR-FAK-YM

    Plasmid

    #25928

    Depositor

    Klaus Hahn

    Article

    Karginov et al Nat Biotechnol. 2010 Jul;28(7):743-7. do

    Insert

    RapR-FAK (Ptk2 Mouse, Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    This pEGFP-RapR-FAK-YM plasmid contains the FAK m…

    Promoter

    Available since

    Oct. 18, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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13. pUC118-mCtnnb1-FLIP-Puro

    Plasmid

    #84540

    Purpose

    CRISPR - FLIP targeting vector for mouse beta-catenin

    Depositor

    Bon-Kyoung Koo

    Article

    Andersson-Rolf et al Nat Methods. 2017 Jan 30. doi: 10.1038/n

    Insert

    mouse beta-catenin FLIP - Puro targeting vector
    

    Use

    Cre/Lox and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pUC118-mCtnnb1-FLIP-FlpE-Puro

    Plasmid

    #84541

    Purpose

    CRISPR - FLIP-FlpE targeting vector for mouse beta-catenin

    Depositor

    Bon-Kyoung Koo

    Article

    Andersson-Rolf et al Nat Methods. 2017 Jan 30. doi: 10.1038/n

    Insert

    mouse beta-catenin FLIP-FlpE - Puro targeting vector
    

    Use

    Cre/Lox and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 31, 2017

    Availability

    Academic Institutions and Nonprofits only

15. pcDNA4-Myc-eIF3f

    Plasmid

    #35975

    Depositor

    Susana Valente

    Article

    Valente et al Proc Natl Acad Sci U S A. 2009 Mar 17;10

    Insert

    eukaryotic translation initiation factor 3, subunit F (EIF3F Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 9, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAPcrRNA_Lmo

    Plasmid

    #126501

    Purpose

    L.monocytogenes CRISPR Type I-B crRNA cloning vector for mammalian expression driven by U6 promoter

    Depositor

    Charles Gersbach

    Article

    Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Sept. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. TET-pLKO.1 PURO shSMAD4 #2

    Plasmid

    #83091

    Purpose

    Lentiviral shRNA vector for inducible knockdown of human SMAD4

    Depositor

    Kevin Janes

    Article

    Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

    Insert

    shSMAD4 (SMAD4 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

18. RapR-Shp2-mVenus-flag

    Plasmid

    #188655

    Purpose

    Rapamycin regulated Shp2 phosphatase with mVenus and flag tag

    Depositor

    Andrei Karginov

    Article

    Fauser et al J Cell Biol. 2022 Aug 1;221(8). pii: 213

    Insert

    Shp2
    

    Use

    Tags

    Flag and mVenus

    Expression

    Mammalian

    Mutation

    iFKBP insertion at V406 with deletion of residues…

    Promoter

    CMV

    Available since

    Aug. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLmoCascade-IL1RNcrRNA

    Plasmid

    #126487

    Purpose

    Encodes L.monocytogenes CRISPR Type I-B crRNA targeting IL1RN

    Depositor

    Charles Gersbach

    Article

    Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

    Insert

    Lmo IL1RN cr03
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Sept. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. All-in-one split-ADAR2

    Plasmid

    #170136

    Purpose

    AAV vector carrying ADAR2-DDC-λN-P2A-MCP-ADAR2-DDN with a nuclear export signal

    Depositor

    Prashant Mali

    Article

    Katrekar et al Elife. 2022 Jan 19;11. pii: 75555. doi:

    Insert

    ADAR2-DDC-λN-P2A-MCP-ADAR2-DDN
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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