We narrowed to 5,507 results for: SPI

1. pcDNA3.1-Flag-PGC-1alpha1

   Plasmid

   #45501

   Depositor

   Bruce Spiegelman, Jorge Ruas

   Article

   Ruas et al Cell. 2012 Dec 7;151(6):1319-31. doi: 10

   Insert

   PGC-1alpha Isoform1 (Ppargc1a Mouse)
   

   Use

   Tags

   6xHis and Flag

   Expression

   Mammalian

   Mutation

   Q339K relative to NM_008904

   Promoter

   Available since

   Aug. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pMXs-IRES-blast 3xFlag-SPNS1(E164K)

   Plasmid

   #198569

   Purpose

   SPNS1(E164K) codon-optimized orf with n-terminal 3xFlag in pMXs-IRES-Blast vector

   Depositor

   Monther Abu-Remaileh

   Article

   Scharenberg et al Sci Adv. 2023 Apr 21;9(16):eadf8966. doi

   Insert

   SPNS1(E164K) with n-terminal 3xFlag (SPNS1 Human)
   

   Use

   Retroviral

   Tags

   3xFlag

   Expression

   Mammalian

   Mutation

   changed glutamic acid E164 to lysine (E164K)

   Promoter

   Available since

   July 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pcDNA216-CoV2-RBD-beta variant

   Plasmid

   #194598

   Purpose

   Mammalian expression of RBD domain from SARS-CoV-2 beta (B.1.351)

   Depositor

   Ben Murrell

   Article

   Sheward et al Cell Rep Med. 2021 Nov 16;2(11):100450.

   Insert

   CoV2-RBD-beta variant (S Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pcDNA3.1-Flag-PGC-1alpha2

   Plasmid

   #45502

   Depositor

   Bruce Spiegelman, Jorge Ruas

   Article

   Ruas et al Cell. 2012 Dec 7;151(6):1319-31. doi: 10

   Insert

   PGC-1alpha Isoform2 (Ppargc1a Mouse)
   

   Use

   Tags

   6xHis and Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pMXs-IRES-blast 3xFlag-SPNS1(R76A)

   Plasmid

   #198570

   Purpose

   SPNS1(R76A) codon-optimized orf with n-terminal 3xFlag in pMXs-IRES-Blast vector

   Depositor

   Monther Abu-Remaileh

   Article

   Scharenberg et al Sci Adv. 2023 Apr 21;9(16):eadf8966. doi

   Insert

   SPNS1(R76A) with n-terminal 3xFlag (SPNS1 Human)
   

   Use

   Retroviral

   Tags

   3xFlag

   Expression

   Mammalian

   Mutation

   changed arginine R76 to alanine (R76A)

   Promoter

   Available since

   July 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pMXs-IRES-blast GFP-SPNS1(E164K)

   Plasmid

   #198572

   Purpose

   SPNS1(E164K) codon-optimized orf with n-terminal 3xFlag GFP in pMXs-IRES-Blast vector

   Depositor

   Monther Abu-Remaileh

   Article

   Scharenberg et al Sci Adv. 2023 Apr 21;9(16):eadf8966. doi

   Insert

   SPNS1(E164K) with n-terminal 3xFlag-GFP fusion (SPNS1 Human)
   

   Use

   Retroviral

   Tags

   3xFlag-GFP

   Expression

   Mammalian

   Mutation

   changed glutamic acid E164 to lysine (E164K)

   Promoter

   Available since

   July 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pcDNA3.1(+)_U6 tRNAPyl_CMV NESPylRS(AF)_IRES_eRF1(E55D)-HA

   Plasmid

   #182653

   Purpose

   Encodes codon-optimized AF mutant of M. mazei pyrrolysine (Pyl) tRNA synthetase fused to a nuclear export signal (NESPylRSAF),tRNACUAPyl & eRF1E55D used for amber codon suppression in mammalian cells

   Depositor

   Ivana Nikić-Spiegel

   Article

   Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:

   Inserts

   codon-optimized Y306A/Y384F (AF) double mutant of Methanosarcina mazei-derived pyrrolysine (Pyl) tRNA synthetase
   PylT
   mutant eukaryotic release factor 1
   

   Use

   Tags

   HA tag and nuclear export signal (NES)

   Expression

   Mammalian

   Mutation

   E55D and Y306A/Y384F (AF) double mutant of Methan…

   Promoter

   CMV and U6

   Available since

   June 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pcDNA3.3_CoV2_501V2

   Plasmid

   #170449

   Purpose

   Express SARS-CoV-2 501V2 (South Africa strain / beta strain) spike protein with an 18aa deletion on the C-terminal tail. Used for SARS2 501V2 pseudotyped virus production.

   Depositor

   David Nemazee

   Article

   Yuan et al Science. 2021 Aug 13;373(6556):818-823.

   Insert

   SARS-CoV-2 501V2 spike D18 (S SARS-CoV-2, 501V2 (B.1.351))
   

   Use

   Tags

   NA

   Expression

   Mammalian

   Mutation

   L18F, D80A, D215G, R246I, K417N, E484K, N501Y, D6…

   Promoter

   CMV

   Available since

   May 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCDNA3.3_CoV2_B.1.1.7

   Plasmid

   #170451

   Purpose

   Express SARS-CoV-2 B.1.1.7 (UK strain / alpha strain) spike protein with an 18aa deletion on the C-terminal tail. Used for SARS2 (UK strain) pseudotyped virus production.

   Depositor

   David Nemazee

   Article

   Yuan et al Science. 2021 Aug 13;373(6556):818-823.

   Insert

   SARS-CoV-2 501V2 spike D18 (S SARS-CoV-2, UK strain (B.1.1.7))
   

   Use

   Tags

   NA

   Expression

   Mammalian

   Mutation

   HY69-70del, Y144del, N501Y, A570D, D614G, P681H, …

   Promoter

   CMV

   Available since

   May 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA3.3_CoV2_P1

    Plasmid

    #170450

    Purpose

    Express SARS-CoV-2 P.1 (Brazil / gamma) strain spike protein with an 18aa deletion on the C-terminal tail. Used for SARS2 P.1 pseudotyped virus production.

    Depositor

    David Nemazee

    Article

    Yuan et al Science. 2021 Aug 13;373(6556):818-823.

    Insert

    SARS-CoV-2 P.1 spike D18 (S SARS-CoV-2, P.1 strain)
    

    Use

    Tags

    NA

    Expression

    Mammalian

    Mutation

    L18F, T20N, P26S, D138Y, R190S, K417T, E484K, N50…

    Promoter

    CMV

    Available since

    May 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pOPINO_NbH11-H4

    Plasmid

    #155364

    Purpose

    Expresses nanobody H11-H4, which binds to the receptor binding domain (RBD) of the spike protein of SARS-Cov-2

    Depositor

    Ray Owens

    Article

    Huo et al Nat Struct Mol Biol. 2020 Jul 13. pii: 1

    Insert

    H11-H4
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 4, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

12. pOPINO_NbH11-D4

    Plasmid

    #155363

    Purpose

    Expresses nanobody H11-D4, which binds to the receptor binding domain (RBD) of the spike protein of SARS-Cov-2

    Depositor

    Ray Owens

    Article

    Huo et al Nat Struct Mol Biol. 2020 Jul 13. pii: 1

    Insert

    Nb H11-D4
    

    Use

    Tags

    His6

    Expression

    Mutation

    Promoter

    T7

    Available since

    Aug. 4, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

13. pSpp1-is2

    Plasmid

    #58248

    Purpose

    Expression vector producing isoform 2 derived mouse osteopontin (EGFP tagged)

    Depositor

    Georgios Stathopoulos

    Article

    Giannou et al J Clin Invest. 2015 Jun;125(6):2317-34.

    Insert

    Spp1-is2 (Spp1 Mouse)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pSpp1-is3

    Plasmid

    #58247

    Purpose

    Expression vector producing isoform 3 derived mouse osteopontin (EGFP tagged)

    Depositor

    Georgios Stathopoulos

    Article

    Giannou et al J Clin Invest. 2015 Jun;125(6):2317-34.

    Insert

    Spp1 isoform 3 (Spp1 Mouse)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pVRC8400-SARS-CoV-2-S2P-B.1.617.2-AVI

    Plasmid

    #176334

    Purpose

    Expresses SARS-CoV-2 S2P protein from the B.1.617.2 variant with single chain Fc, HRV3C protease cleavage site, and AVI tag

    Depositor

    Peter Kwong

    Article

    Teng et al PLoS One. 2022 May 24;17(5):e0268767. do

    Insert

    SARS-CoV-2-S2P-B.1.617.2 (S )
    

    Use

    Tags

    AVI, HRV3C, and scFc

    Expression

    Mammalian

    Mutation

    T19R, G142D, del156-157, R158G, L452R, T478K, D61…

    Promoter

    Available since

    March 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pVRC8400-SARS-CoV-2-RBD-BA.2-AVI

    Plasmid

    #184533

    Purpose

    Expresses SARS-CoV-2 RBD domain from the BA.2 variant with single chain Fc, HRV3C protease cleavage site, and AVI tag

    Depositor

    Peter Kwong

    Article

    Teng et al PLoS One. 2022 May 24;17(5):e0268767. do

    Insert

    SARS-CoV-2-RBD-BA.2 (S )
    

    Use

    Tags

    AVI, HRV3C, and scFc

    Expression

    Mammalian

    Mutation

    G339D, S371F, S373P, S375F, T376A, D405N, R408S, …

    Promoter

    Available since

    May 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pVRC8400-SARS-CoV-2-RBD-E484K-AVI

    Plasmid

    #176444

    Purpose

    Expresses SARS-CoV-2 RBD domain containing E484K mutation with single chain Fc, HRV3C protease cleavage site, and AVI tag

    Depositor

    Peter Kwong

    Article

    Teng et al PLoS One. 2022 May 24;17(5):e0268767. do

    Insert

    SARS-CoV-2-RBD-E484K (S )
    

    Use

    Tags

    AVI, HRV3C, and scFc

    Expression

    Mammalian

    Mutation

    E484K

    Promoter

    Available since

    March 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pVRC8400-SARS-CoV-2-RBD-SD1-L452R-T478K-AVI

    Plasmid

    #176456

    Purpose

    Expresses SARS-CoV-2 RBD-SD1 domain containing L452R-T478K mutations with single chain Fc, HRV3C protease cleavage site, and AVI tag

    Depositor

    Peter Kwong

    Article

    Teng et al PLoS One. 2022 May 24;17(5):e0268767. do

    Insert

    SARS-CoV-2-RBD-SD1-L452R-T478K (S )
    

    Use

    Tags

    AVI, HRV3C, and scFc

    Expression

    Mammalian

    Mutation

    L452R, T478K

    Promoter

    Available since

    March 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pVRC8400-SARS-CoV-2-S2P-B.1.1.7-AVI

    Plasmid

    #176325

    Purpose

    Expresses SARS-CoV-2 S2P protein from the B.1.1.7 variant with single chain Fc, HRV3C protease cleavage site, and AVI tag

    Depositor

    Peter Kwong

    Article

    Teng et al PLoS One. 2022 May 24;17(5):e0268767. do

    Insert

    SARS-CoV-2-S2P-B.1.1.7 (S )
    

    Use

    Tags

    AVI, HRV3C, and scFc

    Expression

    Mammalian

    Mutation

    69-70 del, 144del, N501Y, A570D, D614G, P681H, T7…

    Promoter

    Available since

    March 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pVRC8400-SARS-CoV-2-NTD-BA.2-AVI

    Plasmid

    #184532

    Purpose

    Expresses SARS-CoV-2 NTD domain from the BA.2 variant with single chain Fc, HRV3C protease cleavage site, and AVI tag

    Depositor

    Peter Kwong

    Article

    Teng et al PLoS One. 2022 May 24;17(5):e0268767. do

    Insert

    SARS-CoV-2-NTD-BA.2 (S )
    

    Use

    Tags

    AVI, HRV3C, and scFc

    Expression

    Mammalian

    Mutation

    T19I, L24-, P25-, P26-, A27S, G142D, V213G

    Promoter

    Available since

    May 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge