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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 302 results for: SPI

Showing: 1 - 20 of 302 results

  2. Antibody Neutralization Response Against Pseudoviruses Expressing SARS-CoV-2 Spike Protein Variants

    Type
    Blog Post
    ...SARS-CoV-2 spike variants. “We created a series of spike expressing plasmids which encode spikes found on... Figure 1: Neutralization levels against spike pseudovirus decreases depending on the variant. ...Garcia-Beltran et al., 2021 with permission.   The spike variant pseudovirus neutralization assay Balazs’..., 2021 with permission.   Mutations in the spike protein are one of the key drivers in determining...

  3. No Llamas Required - Synthetic Nanobodies Against Membrane Proteins

    Type
    Blog Post
    ...Huber S, Siegrist M, Hetemann L, Gera J, Gmür S, Spies P, Gygax D, Geertsma ER, Dawson RJ, Seeger MA (2018... identified nanobodies that bind to coronavirus spike proteins (Wrapp et al., 2020), which sit on the ...enter host cells. To do this, they injected the spike protein from two different coronaviruses into a ...isolated special camelid antibodies that bind to the spike proteins in cell culture, and inhibit these viruses...generate sybodies, synthetic nanobodies against the spike protein (Walter et al., 2020). No llamas required...Receptor Binding Domain found on the membrane-bound spike protein, in a remarkable 12 working days. While ...

  4. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...PRKCG MTH Spinocerebellar ataxia 30 Frederic Mushinski 8418 pLTR PKC gamma PRKCG Spinocerebellar ataxia ...GFP CMV Spinocerebellar ataxia 27 Tobias Meyer 21204 GFP-N2-PKCgamma PRKCG GFP CMV Spinocerebellar ataxia...GFP CMV Spinocerebellar ataxia 25 Tobias Meyer 21215 GFP-C2gamma PRKCG GFP CMV Spinocerebellar ataxia ...CMV Spinocerebellar ataxia 23 Bernard Weinstein 21237 PKC gamma dNPS PRKCG HA CMV Spinocerebellar ataxia... CMV Spinocerebellar ataxia 21 Bernard Weinstein 21239 PKC gamma DN PRKCG HA CMV Spinocerebellar ataxia...CMV Spinocerebellar ataxia 15 Ryohei Yasuda 112270 CMV-PKCg-mEGFP-N1 PRKCG GFP CMV Spinocerebellar ataxia...GFP CMV Spinocerebellar Ataxia Richard Youle 176464 pVPS13D-I-EGFP VPS13D GFP CMV Spinocerebellar Ataxia...

  5. COVID-19 Resources

    Type
    Collection
    ...all spike expression plasmids in our collection that are suitable for generating SARS-CoV-2 spike pseudotyped...Libraries SARS-CoV-2 Spike (S) Ectodomain and RBD Libraries - Libraries of Spike (S) Ectodomain and RBD...SARS-CoV-2 Spike Receptor Binding Domain Deep Mutation Scanning Library - Library of Spike protein RBD...entry driven by the severe acute respiratory syndrome coronavirus spike protein. . PCP4 - Kogiso H, et ...SARS-CoV and MERS CoV, depends on binding of the viral spike (S) protein to cellular receptors. The cellular ...and more. Pooled Libraries : Pooled libraries of spike protein mutations. Research Articles : A list of...contains: Plasmids for E. coli and Yeast expression Spike protein constructs for making VSV pseudo virus Mammalian...

  6. Rinehart Lab Phosphoprotein Reagents

    Type
    Collection
    ...Pooled Library 188526 iSPI_pSer_Subpool#1 Pooled Library 188527 iSPI_pSer_Subpool#2 Pooled Library 188528...188528 iSPI_pSer_Subpool#3 Pooled Library 188529 iSPI_pSer_Subpool#4 Pooled Library 188530 iSPI_pSer_Subpool...Pooled Library 188531 iSPI_pSer_Subpool#6 Pooled Library 188532 iSPI_pSer_Subpool#7 Pooled Library 188533...188533 iSPI_pSer_Subpool#8 Pooled Library 188534 iSPI_pSer_Subpool#9 Pooled Library 188535 iSPI_pSer_Subpool...using iSPI, rEcoli XPS, and pSerOTS-C1* (V70) (ca. 2022) from the Rinehart lab: Methods for iSPI, rEcoli...XpS in the Methods section link below (Methods for iSPI, rEcoli XPS, pSerOTS-C1*). The synthetic human phosphoproteome... Iterative Synthetically Phosphorylated Isomers (iSPI). This new resource described in (Gassaway et al...

  7. Brain Armamentarium

    Type
    Collection
    ...CN4825) Expression of iCre(R297T) in striatal medium spiny neurons Jonathan Ting Viviana Gradinaru 214858-PHPeB...of jGCaMP8s in striatal indirect pathway medium spiny neurons (D2 MSNs) Jonathan Ting Viviana Gradinaru...Expression of jGCaMP8s in striatal direct pathway medium spiny neurons (D1 MSNs) Jonathan Ting Viviana Gradinaru... CoChR-EGFP in striatal indirect pathway medium spiny neurons (D2 MSNs) Jonathan Ting Viviana Gradinaru...of CoChR-EGFP in striatal direct pathway medium spiny neurons (D1 MSNs) Jonathan Ting Viviana Gradinaru...of jRGECO1a in striatal indirect pathway medium spiny neurons (D2 MSNs) Jonathan Ting Viviana Gradinaru...for Cre recombinase expression in striatal medium spiny neurons Jonathan Ting Viviana Gradinaru 199774-PHPeB...

  8. Allen Institute for Brain Science AAV Enhancer Collection

    Type
    Collection
    ...-BGHpA AiP13044 AiE0779m_3xC2 SYFP2 DRD1 Medium spiny neurons (D1-MSN) Striatum 199776 pAAV-AiE0779m_3xC2...R297T)-BGHpA AiP13779 AiE0779m_3xC2 Cre DRD1 Medium spiny neurons (D1-MSN) Striatum 214855 pAAV-AiE0779m_3xC2...minBG-FlpO-WPRE3-BGHpA AiP14201 AiE0779m_3xC2 FlpO DRD1 Medium spiny neurons (D1-MSN) Striatum 224206 pAAV-AiE0780m-minCMV-SYFP2...WPRE3-BGHpA AiP15827 AiE0780m SYFP2 DRD1 Medium spiny neurons (D1-MSN) Striatum 224200 pAAV-AiE0780m-minBG-iCre...(R297T)-BGHpA AiP15578 AiE0780m Cre DRD1 Medium spiny neurons (D1-MSN) Striatum 191707 pAAV-AiE0452h-minBG-SYFP2...WPRE3-BGHpA AiP12237 AiE0452h SYFP2 DRD2 Medium spiny neurons (D2-MSN) Striatum 199777 pAAV-AiE0452h-minBG-iCre...(R297T)-BGHpA AiP13781 AiE0452h Cre DRD2 Medium spiny neurons (D2-MSN) Striatum 230871 pAAV-AiE0452h-minBG-FlpO-WPRE3...

  9. SARS-CoV-2 Pseudotyped Virus

    Type
    Collection
    ...Plasmids Expressing Spike and Spike Variants Full-length wildtype SARS-CoV-2 spike protein is inefficient...plasmids for viral pseudotyping with SARS-CoV-2 spike protein. Collections...replication-defective virus particles with the SARS-CoV-2 spike (S) glycoprotein on the envelope surface. Pseudotyped...particles have been pseudotyped with the SARS-CoV-2 Spike protein and you will find examples of each in Addgene's.... Use the search bar to find a particular viral spike protein (MERS, CoV-1, CoV-2), modification, or variant...Pseudotyping Lentiviral Particles with SARS-CoV-2 Spike Protein Nemazee lab - Pseudotyped virus protocol...Response Against Pseudoviruses Expressing SARS-CoV-2 Spike Protein Variants Addgene COVID-19 Resources and ...

  10. Neurodegeneration Research Collection

    Type
    Collection
    ... upper and lower motor neurons in the brain and spinal cord. Over time, a loss of motor neurons leads ...brain. The cause of this neuron death is unknown. Despite the idiopathic (having no specific known cause)...

  11. Genomic Deletions in Mammalian Cell Lines

    Type
    Collection
    ... 7.3 for suspension cells. For adherent cells, aspirate media. Add 20 μl of 0.05% trypsin-EDTA to each...200 μl. Wait 24 - 72 hr to allow cells to grow. Aspirate media. Add 50 μl DNA extraction solution per well...

  12. Plasmids for Stem Cell Research

    Type
    Collection
    ... of mouse and human fibroblasts into functional spinal motor neurons. Cell Stem Cell. 2011 Sep 2;9(3):...2013 May;31(5):426-33. Tesar Fibroblasts Medium Spiny Neurons Lentiviral Human Generation of Human Striatal...

  13. Genetic Code Expansion

    Type
    Collection
    ... synthetase M. mazei Mammalian TAG Ivana Nikić-Spiegel 182537 pEVOL-MmAcKRS1-PylTUUA PylRS M. mazei Bacterial... synthetase M. mazei Mammalian TAG Ivana Nikić-Spiegel 182881 Mut1-RS tyrosyl-tRNA synthetase M. jannaschii...

  14. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...Serotonin (5-HT) sensor psychLight Psychedelic-inspired drug discovery using an engineered biosensor. ...glutamate receptor Glutamate receptor exocytosis and spine enlargement during chemically induced long-term ...

  16. Adenovirus Plasmids

    Type
    Collection
    ...double-stranded DNA viruses known to cause mild to severe respiratory disease in humans. Advantages of adenoviruses...

  17. p53 Pathway

    Type
    Collection
    ...growth factor binding protein 3 KAI CD82 molecule Maspin Serpin family B member 5 MDM2 MDM2 proto-oncogene...

  18. TALEN Guide

    Type
    Collection
    ...Dr. Keith Joung’s lab at Massachusetts General Hospital also recently released a TALEN kit through Addgene...

  19. Viral Vectors

    Type
    Collection
    ...of our viral pages, we thank Boston Children's Hospital Viral Core . Viral Plasmid Biosafety Resources...

  20. CRISPR References and Information

    Type
    Collection
    ...in Japan. Developed by the Kumiko Ui-Tei Lab . CRISPick (Link opens in a new window) Ranks and picks candidate...
Showing: 1 - 20 of 302 results