We narrowed to 6,524 results for: poly
-
Plasmid#121805PurposepMVP L3-L2 entry plasmid, contains polyA + EF1a-TETa-polyA for 3- or 4-component MultiSite Gateway Pro assembly. Adds EF1a-driven TETa downstream of gene of interest.DepositorInsertpolyA + EF1a::TETa-polyA
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
KA401: pMVP (L3-L2) pA; RIP::TETa-pA
Plasmid#121809PurposepMVP L3-L2 entry plasmid, contains polyA + RIP-TETa-polyA for 3- or 4-component MultiSite Gateway Pro assembly. Adds RIP-driven TETa downstream of gene of interest.DepositorInsertpolyA + RIP::TETa-polyA
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
KO502: pMVP (L3-L2) HA tag + pA; RIP::TETa-pA
Plasmid#121810PurposepMVP L3-L2 entry plasmid, contains HA tag-polyA + RIP-TETa-polyA for 3- or 4-component MultiSite Gateway Pro assembly. Adds C-term HA tag to gene plus downstream RIP-driven TETaDepositorInsertHA epitope tag-polyA + RIP::TETa-polyA
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRevLCav2deltaC
Plasmid#60809Purposeto make truncation of yeast Rev3DepositorInsertREV3 (REV3 Budding Yeast)
UseTagsExpressionYeastMutationrev3ΔC encodes for Rev3 lacking the C-terminus (a…PromoterAvailable sinceMarch 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRevLCav2FeS
Plasmid#60808Purposeto disrupt iron sulfur cluster in yeast Rev3DepositorInsertREV3 (REV3 Budding Yeast)
UseTagsExpressionYeastMutationcontains 647 C-terminal amino acids and 335 bp of…PromoterAvailable sinceMarch 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
SLC39A14-GFP
Plasmid#104380PurposeExpresses SLC39A14/ZIP14 wild-type in mammalian cellsDepositorInsertSLC39A14 (SLC39A14 Human)
UseTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceDec. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJB50
Plasmid#226311PurposeE. coli and H. neapolitanus shuttle vector; integrates the gene of interest and a kanamycin resistance marker into a neutral site on the H. neapolitanus genome.DepositorInsertCsoS2 with V(T/S)G to AAA CsoS2 MR1-7
UseTagsExpressionBacterialMutationVTG273-5AAA, VTG284-6AAA, VTG295-7AAA, VSG332-4AA…PromoterpTRCAvailable sinceOct. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJB55
Plasmid#226310PurposeE. coli and H. neapolitanus shuttle vector; integrates the gene of interest and a kanamycin resistance marker into a neutral site on the H. neapolitanus genome.DepositorInsertCsoS2 with V(T/S)G to AAA CsoS2 MR1-5
UseTagsExpressionBacterialMutationVTG273-5AAA, VTG284-6AAA, VTG295-7AAA, VSG332-4AA…PromoterpTRCAvailable sinceOct. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSP6-RNAP
Plasmid#48143PurposeShuttle vector E. coli/B.meg.; encoding the RNA polymerase of the phage SP6 under control of the xylose inducible promoter PxylADepositorInsertrna polymerase SP6
UseSynthetic BiologyTagsExpressionBacterialMutationV314A (numbering based on NCBI Sequence ID: NP_85…PromoterAvailable sinceOct. 21, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCMV6-AN-HA_WT POLH
Plasmid#201671PurposeExpression of WT DNA polymerase eta with an N-terminal HA tag in mammalian cellsDepositorInsertPolymerase eta (POLH Human)
UseTagsHAExpressionMammalianMutationCoding sequence has been optimised for expression…PromoterCMVAvailable sinceJune 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
L2_lacZgRNA-Cas9-CsA
Plasmid#136138PurposePlasmid to accept gRNA target with SapI, lacZ blue-white screening. Contains Cas9 and HygR.DepositorInsertp5-35Sx2:HygR p5-MpU6:lacZgRNA p5-MpEF1a:Cas9-NLS
UseSynthetic BiologyTagsExpressionPlantMutationBsaI/ SapI domesticatedPromoterAvailable sinceNov. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
HP-Cas9hGem-Ade2-LEU2-LINEAR
Plasmid#174839PurposepRS414 backbone containing LINEAR fragment targeting ADE2 in H. polymorphaDepositorInsertHH-gRNA-HDV targetting ADE2 in Ogataea (para)polymorpha
UseCRISPR and Synthetic BiologyTagsExpressionBacterial and YeastMutationPromoterTDH3pAvailable sinceJan. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
IR904: pMVP (L3-L2) pA; CMV::eGFP-P2A-TETa-pA
Plasmid#121799PurposepMVP L3-L2 entry plasmid, contains polyA + CMV-eGFP-P2A-TETa-polyA for 3- or 4-component MultiSite Gateway Pro assembly. Adds CMV-driven GFP-P2A-TETa downstream of gene of interest.DepositorInsertpolyA + CMV::eGFP-P2A-TETa-polyA
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCOA-Leu-FvPPT1
Plasmid#179923PurposeExpression vector for heterologous expression of a polyketide synthase in S. cerevisiae. Carries and empty insertion site and the Fusarium verticillioides 4´-phosphopantetheinyltransferase PPT1DepositorInsertFvPPT1
UseTagsExpressionYeastMutationCodon optimized for expression in Saccharomyces c…PromoterYarrowia lipolytica EXP1Available sinceNov. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
L2_2xNOP1gRNA-Cas9-CsA
Plasmid#136140PurposePlasmid with 2 different NOP1-gRNA. Targets 500 bp apart in the genome. To tranform Mp as CRISPR control. Contains Cas9 and HygRDepositorInsertp5-35S:HygR p5-MpU6:NOP1gRNA2 p5-MpU6:NOP1gRNA1 p5-MpEF1a:Cas9-NLS
UseSynthetic BiologyTagsExpressionPlantMutationBsaI/ SapI domesticatedPromoterAvailable sinceNov. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
deltaVP1 only
Plasmid#166675PurposeYeast integrative plasmid for expressing deltaVP1, an NLS deletion mutant of Murine polyomavirus VP1 (GAL1 promoter). Contains uracil auxotrophic marker (K. lactis URA3).DepositorInsertMurine polyomavirus deltaVP1 (NLS deletion mutant)
UseSynthetic BiologyTagsExpressionYeastMutationPromoterGAL1Available sinceApril 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
JN511: pMVP (L3-L2) pA; EF1a::eGFP-P2A-TETa-pA
Plasmid#121803PurposepMVP L3-L2 entry plasmid, contains polyA + EF1a-eGFP-P2A-TETa-polyA for 3- or 4-component MultiSite Gateway Pro assembly. Adds EF1a-driven GFP-P2A-TETa downstream of gene of interest.DepositorInsertpolyA + EF1a::eGFP-P2A-TETa-polyA
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
IG806: pMVP (L3-L2) pA; RIP::eGFP-P2A-TETa-pA
Plasmid#121807PurposepMVP L3-L2 entry plasmid, contains polyA + RIP-eGFP-P2A-TETa-polyA for 3- or 4-component MultiSite Gateway Pro assembly. Adds RIP-driven GFP-P2A-TETa downstream of gene of interest.DepositorInsertpolyA + RIP::eGFP-P2A-TETa-polyA
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTreTight-Htt94Q-CFP
Plasmid#23966DepositorInsertN-terminal fragment Huntingtin Q94 (HTT Human)
UseTagsCFPExpressionMammalianMutation94 polyQ repeatPromoterAvailable sinceApril 15, 2010AvailabilityAcademic Institutions and Nonprofits only -
pLenti HsATP13A3 D498N
Plasmid#195850PurposeExpresses D498N mutant Homo sapiens ATP13A3DepositorInsertATPase 13A3 D498N (ATP13A3 Human)
UseLentiviralTagsExpressionMutationPromoterCMVAvailable sinceOct. 16, 2023AvailabilityAcademic Institutions and Nonprofits only