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Showing: 1361 - 1380 of 1402 results

  2. pTU1-A-gap_RiboJ_GFP+_MGApt_Bba_B0015

    Plasmid
    #107577
    Purpose
    B. megaterium DSM319 gap promoter, GFP+ with malachite green mRNA aptamer for Bacillus cell-free transcription-translation. Bacillus shuttle vector backbone, colE1/AmpR (E. coli), RebB/TetA (Bacillus)
    Depositor
    Paul Freemont
    Article
    Moore et al Proc Natl Acad Sci U S A. 2018 Apr 17. p
    Insert
    GFP+
    Use
    Synthetic Biology; E. coli and bacillus shuttle v…
    Tags
    B. megaterium DSM319 xylA leader sequence (MTSSKI…
    Expression
    Bacterial
    Mutation
    F64L/ S65T/ Q80R/ F99S/ M153T/ V163A
    Promoter
    gapdh promoter - B. megaterium DSM319
    Available since
    April 30, 2018
    Availability
    Academic Institutions and Nonprofits only
    107577-plasmid-map-sequence-id-209152

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  3. pB-TR-TTHA

    Plasmid
    #232479
    Purpose
    Tetracycline inducible PiggyBac vector expressing bacterial TTHA1718 (TTHA) gene gene including flag-tag (DYKDDDDK) on the 3' end
    Depositor
    Lukas Trantirek
    Article
    Rynes et al Commun Biol. 2025 Feb 7;8(1):194. doi: 1
    Insert
    Thermus thermophilus HB8
    Use
    Transposon system
    Tags
    DYKDDDDK (FLAG-tag)
    Expression
    Mammalian
    Mutation
    Promoter
    CMV-TetO
    Available since
    May 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    232479-plasmid-map-sequence-id-471676

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  4. AAV-TRE-scFVDnmt3AL_bGHpA

    Plasmid
    #177354
    Purpose
    AAV expression of a fusion protein with scFV, catalytic domain of Dnmt3a and C terminal domain of Dnmt3l from doxycycine-inducible promoter for targeted DNA methylation editing
    Depositor
    Naoki Yamamoto
    Article
    Yamamoto et al Research Square [Preprint] 2021. doi: 10
    Insert
    catalytic domain of DNA Methyltransferase 3 Alpha (Dnmt3a Mouse)
    Use
    AAV
    Tags
    C-terminal domain of mouse Dnmt3l (194–415 aa), P…
    Expression
    Mammalian
    Mutation
    598–908 aa of mouse Dnmt3a
    Promoter
    Tetracycline-dependent promoter
    Available since
    Sept. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    177354-plasmid-map-sequence-id-353614

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  5. pUt-mNF-GFP

    Plasmid
    #199220
    Purpose
    LPUtopia-7 matching RMCE donor plasmid with GFP Negative-Feedback circuit (mNF-GFP). Use BlastR for positive selection and HSV-TK for negative selection. 
    Depositor
    Gabor Balazsi
    Article
    Wan et al Nat Chem Biol. 2023 Jul;19(7):887-899. d
    Insert
    hTetR::P2A::EGFP
    Use
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV D2ir promoter
    Available since
    June 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    199220-plasmid-map-sequence-id-396466

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  9. pTU1-A-fbp_RiboJ_GFP+_MGApt_Bba_B0015

    Plasmid
    #107578
    Purpose
    B. megaterium DSM319 fbp promoter, GFP+ with malachite green mRNA aptamer for Bacillus cell-free transcription-translation. Bacillus shuttle vector backbone, colE1/AmpR (E. coli), RebB/TetA (Bacillus)
    Depositor
    Paul Freemont
    Article
    Moore et al Proc Natl Acad Sci U S A. 2018 Apr 17. p
    Insert
    GFP+
    Use
    Synthetic Biology
    Tags
    B. megaterium DSM319 xylA leader sequence (MTSSKI…
    Expression
    Bacterial
    Mutation
    F64L/ S65T/ Q80R/ F99S/ M153T/ V163A
    Promoter
    fbp promoter Bacillus megaterium DSM319
    Available since
    April 30, 2018
    Availability
    Academic Institutions and Nonprofits only
    107578-plasmid-map-sequence-id-209151

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  12. pB-TR-hCAII

    Plasmid
    #232480
    Purpose
    Tetracycline inducible PiggyBac vector expressing human CAII gene including flag-tag (DYKDDDDK) on the 3' end
    Depositor
    Lukas Trantirek
    Article
    Rynes et al Commun Biol. 2025 Feb 7;8(1):194. doi: 1
    Insert
    Human carbonic anhydrase II (CA2 Human)
    Use
    Transposon system
    Tags
    DYKDDDDK (FLAG-tag)
    Expression
    Mammalian
    Mutation
    Promoter
    CMV-TetO
    Available since
    May 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    232480-plasmid-map-sequence-id-471671

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  15. pInducer10b-HA-KRAS WT CO SR

    Plasmid
    #164926
    Purpose
    Expresses HA tagged, codon Optimized and siRNA-resistant, tetracycline-inducible KRAS wild type sequence
    Depositor
    Ji Luo
    Article
    Yuan et al Cancer Discov. 2014 Oct;4(10):1182-1197.
    Insert
    KRAS proto-oncogene, GTPase (KRAS) (KRAS Human)
    Use
    Lentiviral
    Tags
    HA tag
    Expression
    Mutation
    9 Valine codons are optimized and siRNA targetin…
    Promoter
    Ubc promoter
    Available since
    May 27, 2021
    Availability
    Academic Institutions and Nonprofits only
    164926-plasmid-map-sequence-id-333718

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  18. pInducer10b-HA-KRAS G12V CO SR

    Plasmid
    #164928
    Purpose
    Expresses HA tagged, codon Optimized and siRNA-resistant, tetracycline-inducible KRASG12V sequence
    Depositor
    Ji Luo
    Article
    Yuan et al Cancer Discov. 2014 Oct;4(10):1182-1197.
    Insert
    KRAS proto-oncogene, GTPase (KRAS) (KRAS Human)
    Use
    Lentiviral
    Tags
    HA tag
    Expression
    Mutation
    Glycine 12 changed to valine; 9 Valine codons are…
    Promoter
    Ubc promoter
    Available since
    May 27, 2021
    Availability
    Academic Institutions and Nonprofits only
    164928-plasmid-map-sequence-id-333719

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Showing: 1361 - 1380 of 1402 results