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pUC-hU6-crBACH1-array_EF1a-BFP

Plasmid

#224787

Purpose

BACH1 targeting crRNA array for RfxCas13d expressed from multiple hU6 promoter and reporter BFP protein expressed from EF1a promoter

Depositor

Gabor Balazsi

Article

Wan et al ACS Synth Biol. 2024 Oct 18;13(10):3212-

Insert

crBACH1-1, crBACH1-2, crBACH1-3,

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Promoter

hU6 and hU6-2xTetO

Available Since

Oct. 31, 2024

Availability

Academic Institutions and Nonprofits only

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p5E-hs_Cldn5 (JDW 1120)

Plasmid

#224481

Purpose

Gateway compatible 5' entry clone containing the Human Claudin 5 promoter

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

Claudin-5 promoter

Expression

Mammalian

Available Since

Sept. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pSuper DGK alfa human

Plasmid

#224574

Purpose

To downregulate the expression of human DGK alfa in human cells

Depositor

Isabel Mérida

Article

Avila-Flores et al Immunol Cell Biol. 2017 Jul;95(6):549-56

Insert

sh RNAi Diacylglycerol kinase alfa human (DGKA Human)

Use

RNAi

Promoter

H1

Available Since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pSuper DGK alfa mouse

Plasmid

#224573

Purpose

Negative Control for expression downregulation of DGK alfa human in human cells

Depositor

Isabel Mérida

Article

Avila-Flores et al Immunol Cell Biol. 2017 Jul;95(6):549-56

Insert

sh RNAi Diacylglycerol kinase alfa mouse (Dgka Mouse)

Use

RNAi

Promoter

H1

Available Since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pWZL-hygro-FAKS722A

Plasmid

#216545

Purpose

This retroviral plasmid expresses a mutated cDNA (dominant negative: S722A) of human PTK2

Depositor

Georgia Konstantinidou

Article

Pozzato et al EMBO Mol Med. 2024 Sep 13. doi: 10.1038/

Insert

PTK2 (S722A) (PTK2 Human)

Use

Retroviral

Tags

No

Expression

Mammalian

Mutation

FAK dominant negative mutation S722A

Available Since

Sept. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pWZL-hygro-FAKY861F

Plasmid

#216546

Purpose

This retroviral plasmid expresses a mutated cDNA (dominant negative: Y861F) of human PTK2

Depositor

Georgia Konstantinidou

Article

Pozzato et al EMBO Mol Med. 2024 Sep 13. doi: 10.1038/

Insert

PTK2 (Y861F) (PTK2 Human)

Use

Retroviral

Tags

No

Expression

Mammalian

Mutation

FAK dominant negative mutation Y861F

Available Since

Sept. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pWZL-hygro-FAKS732A

Plasmid

#216541

Purpose

This retroviral plasmid expresses a mutated cDNA (dominant negative: S732A) of human PTK2

Depositor

Georgia Konstantinidou

Article

Pozzato et al EMBO Mol Med. 2024 Sep 13. doi: 10.1038/

Insert

PTK2 (S732A) (PTK2 Human)

Use

Retroviral

Tags

No

Expression

Mammalian

Mutation

FAK dominant negative mutation S732A

Available Since

Sept. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pWZL-hygro-FAKS910A

Plasmid

#216542

Purpose

This retroviral plasmid expresses a mutated cDNA (dominant negative: S910A) of human PTK2

Depositor

Georgia Konstantinidou

Article

Pozzato et al EMBO Mol Med. 2024 Sep 13. doi: 10.1038/

Insert

PTK2 (S910A) (PTK2 Human)

Use

Retroviral

Tags

No

Expression

Mammalian

Mutation

FAK dominant negative mutation S910A

Available Since

Sept. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pAPM-miR30-DDX17-ts2

Plasmid

#174239

Purpose

DDX17 knockdown

Depositor

Jeremy Luban

Article

Guney et al Proc Natl Acad Sci U S A. 2024 Jul 16;12

Insert

DDX17 shRNA (DDX17 Human)

Use

Lentiviral and RNAi

Promoter

SFFV

Available Since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pGGI_Myr-YFP-MiniTurboID

Plasmid

#222432

Purpose

Potential plasma membrane control.

Depositor

Dominique Bergmann

Article

Wallner et al Dev Cell. 2024 May 6;59(9):1096-1109.e5.

Insert

MiniTurboID (BirA mutant)

Use

Golden gate / green gate compatible cloning vector

Tags

Linker, Myr, and YFP

Mutation

aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

Available Since

July 29, 2024

Availability

Academic Institutions and Nonprofits only

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