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Showing: 181 - 210 of 1105 results

  8. PTEN-VC

    Plasmid
    #89332
    Purpose
    expresses hPTEN fused to the C-terminal part of Venus
    Depositor
    Tiago Outeiro
    Insert
    PTEN1 (PTEN Human)
    Use
    Tags
    C-terminal part of Venus
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Oct. 25, 2017
    Availability
    Academic Institutions and Nonprofits only
    89332-plasmid-map-sequence-id-176937

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  9. pTRE3G-VP64-dCas9-VP64-ZsG

    Plasmid
    #192668
    Purpose
    Dox-inducible expression of VP64-dCas9-VP64 with 2A ZsGreen1
    Depositor
    Jeffrey Miner
    Insert
    VP64-dCas9-VP64, ZsGreen1
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    D10A and N863A in Cas9
    Promoter
    TRE3G
    Available since
    Dec. 15, 2022
    Availability
    Academic Institutions and Nonprofits only
    192668-plasmid-map-sequence-id-379892

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  10. Lenti-EF1alpha-VP64-dCas9-VP64-ZsG

    Plasmid
    #192667
    Purpose
    3rd generation lenti vector encoding VP64-dCas9-VP64 with 2A ZsGreen1
    Depositor
    Jeffrey Miner
    Insert
    VP64-dCas9-VP64, ZsGreen1
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    D10A and N863A in Cas9
    Promoter
    EF1a
    Available since
    Dec. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    192667-plasmid-map-sequence-id-379870

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  11. pTRE3G-dCas9-VP64-MS2-VP64-ZsG

    Plasmid
    #192669
    Purpose
    Dox-inducible expression of dCas9-VP64 with P2A MS2-VP64 and T2A ZsGreen1
    Depositor
    Jeffrey Miner
    Insert
    dCas9-VP64, MS2-VP64, ZsGreen1
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    D10A and N863A in Cas9
    Promoter
    TRE3G
    Available since
    Dec. 15, 2022
    Availability
    Academic Institutions and Nonprofits only
    192669-plasmid-map-sequence-id-379887

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  12. Lenti-EF1alpha-dCas9-VP64-MS2-VP64-ZsG

    Plasmid
    #192665
    Purpose
    3rd generation lenti vector encoding dCas9-VP64 with P2A MS2-VP64 and T2A ZsGreen1
    Depositor
    Jeffrey Miner
    Insert
    dCas9-VP64, MS2-VP64, ZsGreen1
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    D10A and N863A in Cas9
    Promoter
    EF1a
    Available since
    Dec. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    192665-plasmid-map-sequence-id-379871

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  13. pALPS-ZsGreen-miR-146a

    Plasmid
    #89174
    Purpose
    encodes human/murine miR-146a and ZsGreen1
    Depositor
    Hilary Sheppard
    Insert
    miR-146a (MIR146A, Mir146 Mouse, Human)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    SFFVp
    Available since
    April 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    89174-plasmid-map-sequence-id-176064

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  21. pSC50

    Plasmid
    #104824
    Purpose
    CRISPR/Cas9 2xplex gRNA targeting Glyma.08g081600, Glyma.05g126600 (Hen1ab). Also expresses Cas9 from Gmubi promoter.
    Depositor
    Robert Stupar
    Article
    Curtin et al Plant Biotechnol J. 2017 Oct 31. doi: 10
    Insert
    Glyma.08g081600, Glyma.05g126600
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Feb. 7, 2018
    Availability
    Academic Institutions and Nonprofits only
    104824-plasmid-map-sequence-id-203137

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  22. pDM097

    Plasmid
    #216819
    Purpose
    Aspergillus nidulans codon-adjusted mStayGold fluorescent protein, includes linker for N-terminal or internal tagging.
    Depositor
    Ken Sawin
    Article
    Modaffari et al Wellcome Open Res. 2024 Oct 17;9:602. do
    Insert
    mStayGold
    Use
    Tags
    FLAG-(SGGS)x2-XTEN16-(GGGGS)x3 and c4-(GGGGS)x2-X…
    Expression
    Bacterial
    Mutation
    Aspergillus nidulans codon-adjusted
    Promoter
    Available since
    Sept. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    216819-plasmid-map-sequence-id-430144

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  26. pTOPO-col10a1-KI-donor

    Plasmid
    #184874
    Purpose
    Donor template for knock-in of p2a-CreERT2 into the medaka col10a1 locus via CRISPR/Cas9-mediated homology directed repair
    Depositor
    Christoph Winkler
    Article
    Hui et al Development. 2022 May 20. pii: 275483. d
    Inserts
    Collagen10a1 5' homology arm
    Collagen10a1 3' homology arm
    p2a-CreERT2
    Myosin light chain 2 promoter
    eGFP
    Use
    CRISPR and Cre/Lox
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    184874-plasmid-map-sequence-id-363155

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  28. pADC10-SETXhel

    Plasmid
    #193056
    Purpose
    Baculovirus expression of human Senataxin helicase domain for protein production
    Depositor
    Andrew Deans
    Article
    Hodson et al Cell Rep. 2022 Dec 6;41(10):111749. doi:
    Insert
    Senataxin (SETX Human)
    Use
    Tags
    Flag
    Expression
    Insect and Mammalian
    Mutation
    codon optimized for insect cell expression
    Promoter
    hybrid p10 (insect) and CMV (human) expression
    Available since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    193056-plasmid-map-sequence-id-381199

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  29. PTEN-VC

    Plasmid
    #89332
    Purpose
    expresses hPTEN fused to the C-terminal part of Venus
    Depositor
    Tiago Outeiro
    Insert
    PTEN1 (PTEN Human)
    Use
    Tags
    C-terminal part of Venus
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Oct. 25, 2017
    Availability
    Academic Institutions and Nonprofits only
    89332-plasmid-map-sequence-id-176937

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  30. pALPS-ZsGreen-miR-146a

    Plasmid
    #89174
    Purpose
    encodes human/murine miR-146a and ZsGreen1
    Depositor
    Hilary Sheppard
    Insert
    miR-146a (MIR146A, Mir146 Mouse, Human)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    SFFVp
    Available since
    April 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    89174-plasmid-map-sequence-id-176064

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Showing: 181 - 210 of 1105 results