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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 272 results for: G1

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Showing: 121 - 140 of 272 results

  1. pT5T_Rev7_1-211_SA112_KVK_Rev3_1873-1898

    Plasmid
    #105635
    Purpose
    Expresses mouse monomeric Rev7 and fragment of Rev3 protein (amino acids 1873-1898) from bicistronic plasmid
    Depositor
    Thanos Halazonetis
    Inserts
    Rev7 (Mad2l2 Mouse)
    Rev3l (REV3L Human)
    Expression
    Bacterial
    Mutation
    Mutations: F11S, G12A, V132K, C133V, A135K
    Available since
    March 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    105635-plasmid-map-sequence-id-203205

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  2. pT5T_Rev7_1-211_SA112_KVK_Rev3_1989-2014

    Plasmid
    #105637
    Purpose
    Expresses mouse monomeric Rev7 and fragment of Rev3 protein (amino acids 1989-2014) from bicistronic plasmid
    Depositor
    Thanos Halazonetis
    Inserts
    Rev7 (Mad2l2 Mouse)
    Rev3l (REV3L Human)
    Expression
    Bacterial
    Mutation
    Mutations: F11S, G12A, V132K, C133V, A135K
    Promoter
    T7
    Available since
    March 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    105637-plasmid-map-sequence-id-203819

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  3. pDNR-SpCas9-Gem

    Plasmid
    #80424
    Purpose
    Used for in vitro transcription of SpCas9-Gem mRNA. Cas9 is fused to Geminin peptide and is degraded during G0/G1 phases of the cell-cycle to minimize indels caused by non-homologous end joining
    Depositor
    Sara Howden, Melissa Little
    Publication
    Howden et al Stem Cell Reports. 2016 Sep 13;7(3):508-
    Insert
    SpCas9-Gem
    Use
    CRISPR
    Tags
    Geminin
    Mutation
    codon optimized for human
    Promoter
    T7
    Available since
    Aug. 15, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    80424-plasmid-map-sequence-id-151441

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  6. pGL-p21UTRm1&2

    Plasmid
    #20880
    Depositor
    Robert Blelloch
    Publication
    Wang et al Nat Genet. 2008 Dec . 40(12):1478-83.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    p21 3'UTR site 1 and 2 mutation (Cdkn1a Mouse)
    Use
    Luciferase
    Expression
    Mammalian
    Mutation
    Predicted miRNA binding site 1 (Position ~420) GC…
    Available since
    Aug. 5, 2009
    Availability
    Academic Institutions and Nonprofits only

  9. pDEST-FRT-TO-GFP-PALB2 KR

    Plasmid
    #71107
    Purpose
    mammalian expression of GFP-PALB2 mutant. (first 8 Lysine residues mutated to Arginine)
    Depositor
    Daniel Durocher
    Publication
    Orthwein et al Nature. 2015 Dec 9. doi: 10.1038/nature1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PALB2 (PALB2 Human)
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    first 8 Lysine residues mutated to Arginine (K14-…
    Promoter
    CMV/TO
    Available since
    Dec. 2, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits
    71107-plasmid-map-sequence-id-193376

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  11. PB-NES-ZapCV2-Zinc dead

    Plasmid
    #203850
    Purpose
    Expressing zinc dead sensor NES-ZapCV2-Ala in cytoplasm of mammalian cells
    Depositor
    Amy Palmer
    Publication
    Rakshit et al Cell Rep. 2023 Jun 16;42(6):112656. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ZapCV2-Ala zinc dead sensor
    Tags
    Nuclear Export Signal
    Expression
    Mammalian
    Mutation
    Cys and His in Zap1 is mutated with Ala
    Promoter
    CMV promoter
    Available since
    Sept. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    203850-plasmid-map-sequence-id-402879

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  13. pGL-p21UTRm1

    Plasmid
    #20878
    Depositor
    Robert Blelloch
    Publication
    Wang et al Nat Genet. 2008 Dec . 40(12):1478-83.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    p21 3'UTR site 1 mutation (Cdkn1a Mouse)
    Use
    Luciferase
    Expression
    Mammalian
    Mutation
    Predicted miRNA binding site 1 (Position ~420) GC…
    Available since
    Aug. 5, 2009
    Availability
    Academic Institutions and Nonprofits only

  16. ND4 DdCBE G1

    Plasmid
    #221978
    Purpose
    Expresses mitochondrial cytosine base editor arm in mammalian cells
    Depositor
    Stephen Ekker
    Publication
    Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    COX8A MTS-3xFLAG-TALE NT-T-ND4 TALE G1 repeats-TALE CTD-DddAtox 1397C-UGI-ATP5B 3'UTR
    Use
    CRISPR
    Tags
    3xFLAG
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Sept. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    221978-plasmid-map-sequence-id-438728

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  17. ND4 alphaDdCBE G1

    Plasmid
    #221982
    Purpose
    Expresses mitochondrial cytosine base editor arm in mammalian cells
    Depositor
    Stephen Ekker
    Publication
    Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    COX8A MTS-3xFLAG-TALE NT-alphaN-ND4 TALE G1 repeats-TALE CTD-DddAtox 1397C-UGI-ATP5B 3'UTR
    Use
    CRISPR
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    TALE: Q231R, W232G, S233A
    Promoter
    CMV
    Available since
    Sept. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    221982-plasmid-map-sequence-id-438480

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Showing: 121 - 140 of 272 results