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SFFV-NFKB1-Brd 61

Plasmid

#219040

Purpose

Transcription factor NFKB1 with a specific barcode assigned.

Depositor

Filipe Pereira

Article

Kurochkin et al Cell Syst. 2026 Jan 21;17(1):101457. doi

Insert

NFKB1 (NFKB1 Human)

Use

Lentiviral

Promoter

The spleen focus-forming virus (SFFV) promoter

Available Since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR223_NFKB1_WT

Plasmid

#82149

Purpose

Gateway Donor vector containing NFKB1 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

NFKB1 (NFKB1 Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pJK317

Plasmid

#72472

Purpose

puc118 modified to contain ribosome binding site

Depositor

T. J. Kappock

Type

Empty backbone

Expression

Bacterial

Promoter

lac

Available Since

March 21, 2016

Availability

Academic Institutions and Nonprofits only

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pJK635

Plasmid

#72473

Purpose

pET28a modified to contain TEV protease fusion tag instead of T7 tag

Depositor

T. J. Kappock

Type

Empty backbone

Tags

His6 and TEV protease cleavage site

Expression

Bacterial

Promoter

T7

Available Since

Jan. 29, 2016

Availability

Academic Institutions and Nonprofits only

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CMV IkB alpha

Plasmid

#12333

Depositor

Inder Verma

Insert

IkB alpha (Nfkbia Mouse)

Expression

Mammalian

Available Since

Aug. 17, 2006

Availability

Academic Institutions and Nonprofits only
RCAS IkB alpha WT

Plasmid

#12406

Depositor

Inder Verma

Insert

IkB alpha (Nfkbia Mouse)

Use

Retroviral

Expression

Mammalian

Available Since

Aug. 17, 2006

Availability

Academic Institutions and Nonprofits only
FRP1864

Plasmid

#159307

Purpose

Yeast tagging vector to create gene fusion with yeast codon optimized tdKOkappa under KanMX selection

Depositor

Joerg Stelling

Type

Empty backbone

Expression

Yeast

Available Since

Sept. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pJK515

Plasmid

#72385

Purpose

Produces Escherichia coli glycinamide ribonucleotide formyltransferase 2 (EcPurT)

Depositor

T. J. Kappock

Insert

phosphoribosylglycinamide formyltransferase 2

Tags

His6

Expression

Bacterial

Promoter

T7

Available Since

Feb. 24, 2016

Availability

Academic Institutions and Nonprofits only

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pJK630

Plasmid

#72389

Purpose

Produces Acetobacter aceti 1023 phosphoribosylpyrophosphate synthetase (AaPrsA-insV2)

Depositor

T. J. Kappock

Insert

phosphoribosylpyrophosphate synthetase

Expression

Bacterial

Mutation

inserted Val2

Promoter

T7

Available Since

Feb. 24, 2016

Availability

Academic Institutions and Nonprofits only

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pJK100

Plasmid

#73264

Purpose

Produces Bacillus subtilis phosphoribosylformylglycinamidine synthase small subunit (BsPurS)

Depositor

T. J. Kappock

Insert

phosphoribosylformylglycinamidine synthase subunit PurS

Expression

Bacterial

Promoter

T7

Available Since

Feb. 24, 2016

Availability

Academic Institutions and Nonprofits only

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pJK683

Plasmid

#72378

Purpose

Produces human phosphoribosyl pyrophosphate amidotransferase (PRAT), synthetic gene

Depositor

T. J. Kappock

Insert

phosphoribosyl pyrophosphate amidotransferase (PPAT Human)

Expression

Bacterial

Available Since

March 4, 2016

Availability

Academic Institutions and Nonprofits only

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pJK034

Plasmid

#72383

Purpose

Produces Bacillus subtilis glycinamide ribonucleotide synthetase (PurD)

Depositor

T. J. Kappock, JoAnne Stubbe

Insert

phosphoribosylglycinamide synthetase

Expression

Bacterial

Promoter

T7

Available Since

April 11, 2016

Availability

Academic Institutions and Nonprofits only

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pJK649

Plasmid

#72399

Purpose

Produces Escherichia coli phosphoribosylpyrophosphate amidotransferase (EcPurF)

Depositor

T. J. Kappock

Insert

amidophosphoribosyltransferase

Expression

Bacterial

Promoter

T7

Available Since

Feb. 24, 2016

Availability

Academic Institutions and Nonprofits only

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pJK556

Plasmid

#74277

Purpose

Produces formylglycinamide ribonucleotide amidotransferase, N-terminal His6 tag (H6EcPurL)

Depositor

T. J. Kappock

Insert

formylglycinamidine ribonucleotide synthetase

Tags

His6

Expression

Bacterial

Mutation

changes alanine-1246 to serine, numerous silent m…

Promoter

T7

Available Since

July 26, 2016

Availability

Academic Institutions and Nonprofits only

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pJK210

Plasmid

#73325

Purpose

Produces Janthinobacterium lividum violacein biosynthesis protein VioD

Depositor

T. J. Kappock

Insert

violacein biosynthesis protein VioD

Expression

Bacterial

Promoter

T7

Available Since

Feb. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pJK265

Plasmid

#73598

Purpose

Produces Janthinobacterium lividum violacein biosynthesis protein VioC with a N-terminal His6 tag

Depositor

T. J. Kappock

Insert

violacein biosynthesis protein VioC

Tags

His6

Expression

Bacterial

Promoter

T7

Available Since

Feb. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pJK261

Plasmid

#73323

Purpose

Produces Janthinobacterium lividum violacein biosynthesis protein VioA with a N-terminal His6 tag

Depositor

T. J. Kappock

Insert

violacein biosynthesis protein VioA

Tags

His6

Expression

Bacterial

Promoter

T7

Available Since

Feb. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pJK678

Plasmid

#72376

Purpose

Produces human phosphoribosyl pyrophosphate amidotransferase (PRAT), synthetic gene

Depositor

T. J. Kappock

Insert

phosphoribosyl pyrophosphate amidotransferase (PPAT Human)

Use

Gene synthesis

Promoter

n/a

Available Since

Feb. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pJK196

Plasmid

#73322

Purpose

Produces Janthinobacterium lividum violacein biosynthesis protein VioD with a N-terminal His6 tag

Depositor

T. J. Kappock

Insert

violacein biosynthesis protein VioD

Tags

His6

Expression

Bacterial

Promoter

T7

Available Since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only

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pJK375

Plasmid

#72410

Purpose

Produces Acetobacter aceti 1023 Est13

Depositor

T. J. Kappock

Insert

arylesterase

Expression

Bacterial

Promoter

T7

Available Since

Feb. 25, 2016

Availability

Academic Institutions and Nonprofits only

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