Addgene

We narrowed to 7,579 results for: Lif

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pAG469-LifeAct-redFAST

Plasmid

#160365

Purpose

Expresses LifeAct-redFAST in mammalian cells

Depositor

Arnaud Gautier

Article

Tebo et al Nat Chem Biol. 2020 Aug 10. pii: 10.1038

Insert

LifeAct-RedFAST

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pSP64-Lifeact-GFP

Plasmid

#135601

Purpose

Expression of Lifeact-GFP mRNA for microinjection for visualization of actin in S. purpuratus

Depositor

Mamiko Yajima

Article

Uchida et al Dev Biol. 2018 Sep 1;441(1):19-30. doi:

Insert

Lifeact-GFP

Use

In vitro transcription (mrna synthesis)

Tags

EGFP

Expression

Mutation

Promoter

Unknown

Available since

Jan. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-LifeAct-Venus

Plasmid

#87613

Purpose

Actin-targeted Venus (YFP).

Depositor

Jin Zhang

Article

Hertel et al Cell Rep. 2016 Jan 12;14(2):390-400. doi

Insert

LifeAct-Venus

Use

Tags

17-aa sequence from Abp140 (aka "Lifeact&quo…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 14, 2018

Availability

Academic Institutions and Nonprofits only

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mPA-GFP-Lifeact-7

Plasmid

#54671

Purpose

Localization: Actin, Excitation: 400 / 504, Emission: 515 / 517

Depositor

Michael Davidson

Type

Empty backbone

Use

Tags

mPA-GFP-Lifeact

Expression

Mammalian

Mutation

Promoter

Available since

June 12, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pcDNA5/FRT/TO_Lifeact_STf

Plasmid

#182011

Purpose

CMV overexpression of SNAP-tag fast in mammalian cell lines associated to actin

Depositor

Kai Johnsson

Article

Frei et al ACS Chem Biol. 2022 Jun 17;17(6):1321-13

Insert

Lifeact_STf

Use

Tags

Expression

Mammalian

Mutation

Fast mutation on SNAP-tag E30R

Promoter

CMV

Available since

Dec. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pME-Lifeact-mEos2-NS

Plasmid

#115549

Purpose

Multisite gateway vector for 5' tagging with Lifeact-mEos2. Used to make 115563. Parton lab clone IDA

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

Lifeact-mEos2

Use

Tags

Expression

Mutation

Promoter

Available since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pMT Lifeact-TagBFP

Plasmid

#126664

Purpose

Inducible expression of Lifeact-TagBFP in S2 cells

Depositor

Ron Vale

Article

Williamson et al J Cell Biol. 2018 Nov 5;217(11):3977-399

Insert

Lifeact-TagBFP fusion

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 19, 2021

Availability

Academic Institutions and Nonprofits only

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pFX-Lifeact-mCherry

Plasmid

#175822

Purpose

Express mCherry with actin-targeting sequence in mammalian cells

Depositor

Yusuke Ohba

Article

Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

Insert

mCherry with actin-targeting sequence

Use

Tags

Lifeact (N-terminal 17 amino acids from S. Cerevi…

Expression

Mammalian

Mutation

Promoter

CMB

Available since

Feb. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti-Lifeact mApple

Plasmid

#187687

Purpose

In vivo visualization of actin

Depositor

Mary Beckerle

Article

Smith et al PLoS One. 2013 Aug 21;8(8):e69378. doi:

Insert

Lifeact

Use

Lentiviral

Tags

mApple

Expression

Mammalian

Mutation

WT

Promoter

CMV

Available since

Sept. 13, 2022

Availability

Academic Institutions and Nonprofits only

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Lifeact-rsLOV2 pcDNA3.1(+)

Plasmid

#107882

Purpose

Mammalian expression of Lifeact-rsLOV2

Depositor

Stefan Hell

Article

Gregor et al Sci Rep. 2018 Feb 9;8(1):2724. doi: 10.1

Insert

rsLOV2

Use

Tags

Lifeact

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 3, 2018

Availability

Academic Institutions and Nonprofits only

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LifeAct-LSS-SGFP2-N1

Plasmid

#112938

Purpose

Visualization of F-actin

Depositor

Dorus Gadella

Insert

LifeAct

Use

Tags

LSS-SGFP2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 26, 2018

Availability

Academic Institutions and Nonprofits only

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LifeAct-TagBFP-DiB-RM

Plasmid

#168782

Purpose

Actin filament reporter, LifeAct, fused with blue fluorescent protein TagBFP and fluorogen activating protein (FAP) DiB-RM

Depositor

Jens Meiler

Article

Bozhanova et al PLoS Comput Biol. 2021 Nov 8;17(11):e100

Insert

LifeAct-TagBFP-DiB-RM

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 12, 2021

Availability

Academic Institutions and Nonprofits only

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actb2-lifeact-mKate2

Plasmid

#109750

Purpose

Ubiquitous zebrafish expression of mKate2 fused to the actin binding lifeact sequence

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

mKate2 fused to the actin binding lifeact sequence

Use

Zebrafish plasmids

Tags

Expression

Mutation

Promoter

Available since

July 18, 2023

Availability

Academic Institutions and Nonprofits only

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pLNCX2-lifeact-mAPPLE

Plasmid

#89820

Purpose

mAPPLE labeled fluorescent actin

Depositor

Shengyu Yang

Article

Sun et al J Cell Biol. 2014 Nov 24;207(4):535-48.

Insert

lifeact-mAPPLE

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

June 13, 2017

Availability

Academic Institutions and Nonprofits only

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pFX-Lifeact-iRFP

Plasmid

#175823

Purpose

Express iRFP with actin-targeting sequence in mammalian cells

Depositor

Yusuke Ohba

Article

Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

Insert

iRFP with actin-targeting sequence

Use

Tags

Lifeact (N-terminal 17 amino acids from S. Cerevi…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pMpGWB403-Lifeact-Citrine

Plasmid

#100582

Purpose

Binary vector for expression of Citrine-fused Lifeact (Lifeact-Citrine) to visualize actin filaments in plants

Depositor

Yutaka Kodama

Article

Kimura et al PeerJ. 2016 Sep 28;4:e2513. eCollection

Insert

Lifeact-Citrine

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Feb. 5, 2020

Availability

Academic Institutions and Nonprofits only

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pDONR207-Lifeact-Citrine

Plasmid

#100590

Purpose

Gateway entry vector for Lifeact-Citrine

Depositor

Yutaka Kodama

Article

Kimura et al PeerJ. 2016 Sep 28;4:e2513. eCollection

Insert

Lifeact-Citrine

Use

Tags

Expression

Mutation

Promoter

Available since

Feb. 5, 2020

Availability

Academic Institutions and Nonprofits only

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pFX-Lifeact-Venus

Plasmid

#174478

Purpose

Express Venus with actin-targeting sequence in mammalian cells

Depositor

Yusuke Ohba

Article

Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

Insert

Venus with actin-targeting sequence

Use

Tags

Lifeact (N-terminal 17 amino acids from S. Cerevi…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pAG470 LifeAct-iFAST

Plasmid

#130821

Purpose

Expresses LifeAct-iFAST in mammalian cells (actin labeling)

Depositor

Arnaud Gautier

Article

Benaissa et al Nat Commun. 2021 Nov 30;12(1):6989. doi:

Insert

LifeAct-iFAST

Use

Tags

myc-tag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 13, 2019

Availability

Academic Institutions and Nonprofits only

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LifeAct-mScarletI pDRF1-GW

Plasmid

#168085

Purpose

Expresses Lifeact-mScarletI to visualize actin in yeast

Depositor

Bas Teusink

Insert

LifeAct-mScarletI

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

June 18, 2021

Availability

Academic Institutions and Nonprofits only

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