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Plasmid#146728PurposeBacterial Expression of DmAgo1_572-984-optimDepositorInsertDmAgo1_572-984-optim (AGO1 Fly)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28c(plus)-DmAGO1-RC_F
Plasmid#146262PurposeBacterial Expression of DmAGO1-RCDepositorInsertDmAGO1-RC (AGO1 Fly)
UseTagsExpressionBacterialMutationone non silent mutation R787C compared to sequenc…PromoterAvailable sinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmAGO2-RC_B
Plasmid#145980PurposeInsect Expression of DmAGO2-RCDepositorInsertDmAGO2-RC (AGO2 Fly)
UseTagsExpressionInsectMutationA deletion of AA 51-56 and an insertion of 23 AA …PromoterAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmAGO2-RC_B
Plasmid#145981PurposeInsect Expression of DmAGO2-RCDepositorInsertDmAGO2-RC (AGO2 Fly)
UseTagsExpressionInsectMutationA deletion of AA 51-56 and an insertion of 23 AA …PromoterAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmAGO1-RB_B
Plasmid#145977PurposeInsect Expression of DmAGO1-RBDepositorInsertDmAGO1-RB (AGO1 Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmAGO1-F2V2_A
Plasmid#145896PurposeInsect Expression of DmAGO1-F2V2DepositorInsertDmAGO1-F2V2 (AGO1 Fly)
UseTagsExpressionInsectMutationvariation with 2 non silent mutations compared to…PromoterAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmAGO1-F594V_A
Plasmid#145897PurposeInsect Expression of DmAGO1-F594VDepositorInsertDmAGO1-F594V (AGO1 Fly)
UseTagsExpressionInsectMutationvariation with 2 non silent mutations compared to…PromoterAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmAGO1-siRNAres_A
Plasmid#145899PurposeInsect Expression of DmAGO1-siRNAresDepositorInsertDmAGO1-siRNAres (AGO1 Fly)
UseTagsExpressionInsectMutationvariation with 2 non silent mutations compared to…PromoterAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmAGO1-K690A_M
Plasmid#146957PurposeInsect Expression of DmAGO1-K690ADepositorInsertDmAGO1-K690A (AGO1 Fly)
UseTagsExpressionInsectMutationvariation with 2 non silent mutations V315A and H…PromoterAvailable sinceFeb. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmAGO1-F629V_A
Plasmid#145898PurposeInsect Expression of DmAGO1-F629VDepositorInsertDmAGO1-F629V (AGO1 Fly)
UseTagsExpressionInsectMutationV315A, F629V, H913R - variation with 2 non silent…PromoterAvailable sinceMay 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSG5HA-MAGE-112-429
Plasmid#89131Purposemammalian expression of N-terminal HA-tagged human MAGE-A11 amino acid residues 112-429DepositorInsertMAGE-A11 (MAGEA11 Human)
UseTagsHAExpressionMammalianMutationcontains aa 112-429PromoterSV40Available sinceApril 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
FLAG-MAGE-112-429
Plasmid#89128Purposemammalian expression of human MAGE-A11 amino acid residues 112-429 with N-terminal FLAG tagDepositorInsertMAGE-A11 (MAGEA11 Human)
UseTagsFLAGExpressionMammalianMutationcontains aa 112-429PromoterCMVAvailable sinceApril 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pORTMAGE311B
Plasmid#120418PurposeExpresses Lambda Beta and a dominant negative MutL E32K allele, controlled by XylS-Pm expression system for high precision and efficient MAGE experiments at 37°C. RSF1010 Ori; Broad host-range; KanRDepositorArticleInsertsMutL E32K
Lambda Beta
XylS
UseTagsExpressionBacterialMutationE32K mutation conferring dominant mutator phenoty…PromoterPmAvailable sinceFeb. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSin_EF1a_tagBFP_P2A_NLS_pMag_CreC60_IRES_PuroR
Plasmid#176311PurposeExpresses NLS-pMag-CreC60 with tagBFP markerDepositorInserttagBFP_P2A_NLS_pMag_CreC60_IRES_PuroR
UseLentiviral and Synthetic BiologyTagstagBFPExpressionMammalianMutationPromoterhuman EF1alphaAvailable sinceFeb. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pORTMAGE-3
Plasmid#72678PurposeExpresses Lambda Red recombinases and a dominant negative MutL allele all controlled by temperature sensitve cI857 repressor for high precision and efficiency MAGE experiments. Kan resistance marker.DepositorInsertmutL E32K
UseTagsnoneExpressionBacterialMutationE32K mutation conferring dominant mutator phenoty…PromoterAvailable sinceFeb. 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
pORTMAGE-4
Plasmid#72679PurposeExpresses Lambda Red recombinases and a dominant negative MutL allele all controlled by temperature sensitve cI857 repressor for high precision and efficiency MAGE experiments. Cam resistance marker.DepositorInsertmutL E32K
UseTagsnoneExpressionBacterialMutationE32K mutation conferring dominant mutator phenoty…PromoterAvailable sinceFeb. 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
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pORTMAGE312B
Plasmid#128969PurposeBroad-host-range KanR plasmid expressing PapRecT and EcMutL_E32K with Toluic acid inductionDepositorInsertsPapRecT
mutL E32K
UseTagsExpressionBacterialMutationE32KPromoterxylS-PmAvailable sinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pORTMAGE-2
Plasmid#72677PurposeExpresses Lambda Red recombinases and a dominant negative MutL allele all controlled by temperature sensitve cI857 repressor for high precision and efficiency MAGE experiments. Ap resistance marker.DepositorInsertmutL E32K
UseTagsnoneExpressionBacterialMutationE32K mutation conferring dominant mutator phenoty…PromoterpL promoterAvailable sinceFeb. 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCMVMagFRET-1
Plasmid#50742PurposeExpresses MagFRET-1 in the cytosol of mammalian cells. FRET sensor based on Cerulean/Citrine for Mg2+ imaging. Sensor does not respond to normal cytosolic Ca2+ levels.DepositorInsertgene encoding for MagFRET-1
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 16, 2014AvailabilityAcademic Institutions and Nonprofits only