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pCD5-H3TX12-CO-GCN4-TEV-sfGFP-TS

Plasmid

#158739

Purpose

Expresses influenza A virus trimeric hemagglutinin A/H3/TX/12 fused to a sfGFP

Depositor

Robert de Vries

Article

van der Woude et al Protein Sci. 2020 Jul 25. doi: 10.1002/p

Insert

Hemagglutinin A/TX/50/12 H3

Tags

GCN4-TEV-sfGFP-TwinStrep

Expression

Mammalian

Mutation

G186V S219F

Promoter

CMV

Available Since

Nov. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pCD5-TS-sfGFP-Tetrabrachion-TEV-N2NL16

Plasmid

#158742

Purpose

Expresses influenza A virus tetrameric neuraminidase fused to a sfGFP

Depositor

Robert de Vries

Article

van der Woude et al Protein Sci. 2020 Jul 25. doi: 10.1002/p

Insert

Neuraminidase A/NL/354/16 H3N2

Tags

TwinStrep_sfGFP_tetrabrachion

Expression

Mammalian

Mutation

AA61-469

Promoter

CMV

Available Since

Nov. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pCD5-H3CH13-CO-GCN4-TEV-sfGFP-TS

Plasmid

#158740

Purpose

Expresses influenza A virus trimeric hemagglutinin A/H3/CH/13 fused to a sfGFP

Depositor

Robert de Vries

Article

van der Woude et al Protein Sci. 2020 Jul 25. doi: 10.1002/p

Insert

Hemagglutinin A/MI/45/15 H1N1

Tags

GCN4-TEV-sfGFP-TwinStrep

Expression

Mammalian

Mutation

AA61-469

Promoter

CMV

Available Since

Nov. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pCD5-TS-sfGFP-Tetrabrachion-TEV-N2NL03

Plasmid

#158793

Purpose

Expresses influenza A virus tetrameric neuraminidase fused to a sfGFP

Depositor

Robert de Vries

Article

van der Woude et al Protein Sci. 2020 Jul 25. doi: 10.1002/p

Insert

Neuraminidase A/NL/109/03

Tags

TwinStrep_sfGFP_tetrabrachion

Expression

Mammalian

Mutation

AA61-469

Promoter

CMV

Available Since

Nov. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pCD5-TS-sfGFP-Tetrabrachion-TEV-N2NL03

Plasmid

#158793

Purpose

Expresses influenza A virus tetrameric neuraminidase fused to a sfGFP

Depositor

Robert de Vries

Article

van der Woude et al Protein Sci. 2020 Jul 25. doi: 10.1002/p

Insert

Neuraminidase A/NL/109/03

Tags

TwinStrep_sfGFP_tetrabrachion

Expression

Mammalian

Mutation

AA61-469

Promoter

CMV

Available Since

Nov. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-PA-B/BRISBANE/60/2008

Plasmid

#187920

Purpose

Expresses PA subunit of Influenza B/Brisbane/60/2008 virus polymerase in mammalian cells

Depositor

Arindam Mondal

Article

Kedia et al Front Microbiol. 2022 May 25;13:868367.

Insert

polymerase acidic protein

Tags

UNTAGGED

Expression

Mammalian

Promoter

CMV Promoter

Available Since

May 9, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-PB1-B/BRISBANE/60/2008

Plasmid

#187919

Purpose

Expresses PB1 subunit of Influenza B/Brisbane/60/2008 virus polymerase in mammalian cells

Depositor

Arindam Mondal

Article

Kedia et al Front Microbiol. 2022 May 25;13:868367.

Insert

polymerase basic protein 1

Tags

UNTAGGED

Promoter

CMV Promoter

Available Since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-PB2-B/BRISBANE/60/2008

Plasmid

#187917

Purpose

Expresses PB2 subunit of Influenza B/Brisbane/60/2008 virus polymerase in mammalian cells

Depositor

Arindam Mondal

Article

Kedia et al Front Microbiol. 2022 May 25;13:868367.

Insert

polymerase basic protein 2

Tags

UNTAGGED

Promoter

CMV Promoter

Available Since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-PA-B/BRISBANE/60/2008

Plasmid

#187920

Purpose

Expresses PA subunit of Influenza B/Brisbane/60/2008 virus polymerase in mammalian cells

Depositor

Arindam Mondal

Article

Kedia et al Front Microbiol. 2022 May 25;13:868367.

Insert

polymerase acidic protein

Tags

UNTAGGED

Expression

Mammalian

Promoter

CMV Promoter

Available Since

May 9, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-PB1-B/BRISBANE/60/2008

Plasmid

#187919

Purpose

Expresses PB1 subunit of Influenza B/Brisbane/60/2008 virus polymerase in mammalian cells

Depositor

Arindam Mondal

Article

Kedia et al Front Microbiol. 2022 May 25;13:868367.

Insert

polymerase basic protein 1

Tags

UNTAGGED

Promoter

CMV Promoter

Available Since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pKLBac-Bat-FluA-trimer

Plasmid

#229858

Purpose

Influenza A/little yellow-shouldered bat/Guatemala/060/2010(H17N10) polymerase heterotrimer (self-cleaving polyprotein)

Depositor

Stephen Cusack

Article

Pflug et al Nature. 2014 Dec 18;516(7531):355-60. do

Inserts

PA_Bat-FluA_AFC35437.1
PB1_Bat-FluA_AFC35436
PB2_Bat-FluA_AFC35435.1

Expression

Insect

Available Since

Feb. 25, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3-PB2-B/BRISBANE/60/2008

Plasmid

#187917

Purpose

Expresses PB2 subunit of Influenza B/Brisbane/60/2008 virus polymerase in mammalian cells

Depositor

Arindam Mondal

Article

Kedia et al Front Microbiol. 2022 May 25;13:868367.

Insert

polymerase basic protein 2

Tags

UNTAGGED

Promoter

CMV Promoter

Available Since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pHflu4

Plasmid

#203882

Purpose

pHI1 with insertion of monomeric red fluorescent protein (mRFP)

Depositor

Bogumil Karas

Insert

monomeric red fluorescent protein

Use

Synthetic Biology

Expression

Bacterial and Yeast

Available Since

Dec. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-PA-3x-FLAG-B/BRISBANE/60/2008

Plasmid

#187922

Purpose

Expresses C terminal 3xFLAG tagged PA subunit of Influenza B/Brisbane/60/2008 virus polymerase in mammalian cells

Depositor

Arindam Mondal

Article

Kedia et al Front Microbiol. 2022 May 25;13:868367.

Insert

polymerase acidic protein

Tags

3xFLAG

Promoter

CMV Promoter

Available Since

Sept. 23, 2022

Availability

Academic Institutions and Nonprofits only

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Epsin1 deltaUIM-ECFP

Plasmid

#20973

Depositor

Xiaowei Zhuang

Article

Chen et al Proc Natl Acad Sci U S A. 2008 Aug 19. 1

Insert

Epsin1 deltaUIM (Epn1 Rat)

Tags

ECFP

Expression

Mammalian

Available Since

June 15, 2009

Availability

Academic Institutions and Nonprofits only
pCD5-D/bovine/Nebraska/9-5/2012-HEFs57aED-GCN4-sfGFP-ST

Plasmid

#175020

Purpose

Expresses influenza D virus trimeric hemagglutinin esterase fusion protein fused to a sfGFP

Depositor

Robert de Vries

Article

Nemanichvili et al Viruses. 2021 Mar 31;13(4). pii: v130405

Insert

D/bovine/Nebraska/9-5/2012

Tags

GCN4-TEV-sfGFP-TwinStrep

Expression

Mammalian

Mutation

s57a esterase knock out mutant

Promoter

CMV

Available Since

Jan. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pCD5-D/bovine/Nebraska/9-5/2012-HEFwtED-GCN4-sfGFP-ST

Plasmid

#175019

Purpose

Expresses influenza D virus trimeric hemagglutinin esterase fusion protein fused to a sfGFP

Depositor

Robert de Vries

Article

Nemanichvili et al Viruses. 2021 Mar 31;13(4). pii: v130405

Insert

D/bovine/Nebraska/9-5/2012

Tags

GCN4-TEV-sfGFP-TwinStrep

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pHflu2

Plasmid

#203880

Purpose

pHI1 with insertion of ampicillin-resistance gene

Depositor

Bogumil Karas

Insert

broad-spectrum class A beta-lactamase TEM-116

Use

Synthetic Biology

Expression

Bacterial and Yeast

Available Since

Dec. 6, 2023

Availability

Academic Institutions and Nonprofits only

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G1S-HA-pCB6

Plasmid

#74373

Purpose

Mammalian expression of a G1S mutant HA from the X:31 strain of influenza virus

Depositor

Judith White

Article

Qiao et al Mol Biol Cell. 1999 Aug;10(8):2759-69.

Insert

HA

Expression

Mammalian

Mutation

G1S

Promoter

CMV

Available Since

April 13, 2016

Availability

Academic Institutions and Nonprofits only
G1V-HA-pCB6

Plasmid

#74372

Purpose

Mammalian expression of a G1V mutant HA from the X:31 strain of influenza virus

Depositor

Judith White

Article

Qiao et al Mol Biol Cell. 1999 Aug;10(8):2759-69.

Insert

HA

Expression

Mammalian

Mutation

G1V

Promoter

CMV

Available Since

April 13, 2016

Availability

Academic Institutions and Nonprofits only