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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 11,419 results for: Pol

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Showing: 1381 - 1400 of 11419 results

  1. Ec dnaN-pET(11a)

    Plasmid
    #237238
    Purpose
    over-express E. coli dnaN (Pol III beta sliding clamp subunit)
    Depositor
    Michael O'Donnell
    Insert
    dnaN (beta) (dnaN E. coli)
    Expression
    Bacterial
    Available since
    April 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    237238-plasmid-map-sequence-id-469258

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  2. TDH3pro-rtTA-tetO7pro-T7pol-pRS413

    Plasmid
    #239293
    Purpose
    Expresses the reverse tetracycline activator (rtTA) and T7 polymerase; Plasmid #1 of the tet-on overexpression system
    Depositor
    Anita Hopper
    Publication
    Nostramo et al Mol Cell. 2025 Feb 20;85(4):726-741.e6.
    Inserts
    T7 polymerase with NLS and P278L
    reverse tetracycline transactivator (rtTA)
    Expression
    Yeast
    Mutation
    Contains M2-SE-G72P mutations, described in Addge…
    Promoter
    TDH3 and tetracycline-responsive element (TRE) an…
    Available since
    June 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    239293-plasmid-map-sequence-id-476828

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  3. Polymerase expression - pCMV-T7-EcKlenow (LM2692)

    Plasmid
    #208963
    Purpose
    Unfused EcKlenow DNA polymerase (-exo), expressed from CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Publication
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    EcKlenow-BPNLS
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    EcKlenow(-exo;D355A/E357A)
    Promoter
    CMV and T7
    Available since
    Nov. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    208963-plasmid-map-sequence-id-415798

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Showing: 1381 - 1400 of 11419 results